Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsN and SO3964 overlap by 4 nucleotides SO3964 and ptsO overlap by 14 nucleotides ptsO and mgtE-2 are separated by 196 nucleotides
SO3963: ptsN - nitrogen regulatory IIA protein (NCBI ptt file), at 4,103,897 to 4,104,340
ptsN
SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191
SO3964
SO3965: ptsO - phosphocarrier protein NPR (NCBI ptt file), at 4,105,178 to 4,105,453
ptsO
SO3966: mgtE-2 - magnesium transporter (NCBI ptt file), at 4,105,650 to 4,107,014
mgtE-2
Position (kb) 4105
4106 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4104.278 kb on + strand, within ptsN at 4104.386 kb on + strand at 4104.394 kb on - strand at 4104.394 kb on - strand at 4104.465 kb on + strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.574 kb on - strand, within SO3964 at 4104.942 kb on - strand, within SO3964 at 4105.286 kb on - strand, within ptsO at 4105.421 kb on + strand, within ptsO at 4105.443 kb on - strand at 4105.654 kb on - strand at 4105.680 kb on - strand at 4105.906 kb on + strand, within mgtE-2 at 4105.914 kb on - strand, within mgtE-2 at 4106.006 kb on + strand, within mgtE-2 at 4106.048 kb on + strand, within mgtE-2 at 4106.056 kb on - strand, within mgtE-2 at 4106.082 kb on - strand, within mgtE-2 at 4106.165 kb on - strand, within mgtE-2 at 4106.223 kb on - strand, within mgtE-2 at 4106.223 kb on - strand, within mgtE-2
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 4,104,278 + ptsN SO3963 0.86 -1.9 4,104,386 + +1.1 4,104,394 - +1.0 4,104,394 - +1.4 4,104,465 + SO3964 0.15 -0.6 4,104,473 - SO3964 0.16 -2.6 4,104,473 - SO3964 0.16 +0.0 4,104,574 - SO3964 0.28 +1.6 4,104,942 - SO3964 0.71 +0.4 4,105,286 - ptsO SO3965 0.39 -2.4 4,105,421 + ptsO SO3965 0.88 -0.4 4,105,443 - +0.7 4,105,654 - -1.0 4,105,680 - -0.2 4,105,906 + mgtE-2 SO3966 0.19 +0.3 4,105,914 - mgtE-2 SO3966 0.19 -0.4 4,106,006 + mgtE-2 SO3966 0.26 -1.5 4,106,048 + mgtE-2 SO3966 0.29 -0.2 4,106,056 - mgtE-2 SO3966 0.30 +0.5 4,106,082 - mgtE-2 SO3966 0.32 -0.1 4,106,165 - mgtE-2 SO3966 0.38 +0.0 4,106,223 - mgtE-2 SO3966 0.42 -0.9 4,106,223 - mgtE-2 SO3966 0.42 -2.8
Or see this region's nucleotide sequence