Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutY and SO3369 are separated by 9 nucleotides SO3369 and SO3370 are separated by 80 nucleotides SO3370 and SO3371 are separated by 4 nucleotides SO3371 and tRNA-Phe-3 are separated by 387 nucleotides tRNA-Phe-3 and tRNA-Thr-5 are separated by 21 nucleotides
SO3368: mutY - A/G-specific adenine glycosylase (NCBI ptt file), at 3,510,656 to 3,511,753
mutY
SO3369: SO3369 - conserved hypothetical protein (NCBI ptt file), at 3,511,763 to 3,512,041
SO3369
SO3370: SO3370 - conserved hypothetical protein (NCBI ptt file), at 3,512,122 to 3,512,697
SO3370
SO3371: SO3371 - hypothetical Cytochrome B561 (NCBI ptt file), at 3,512,702 to 3,513,253
SO3371
SO_t079: tRNA-Phe-3 - tRNA-Phe (RefSeq), at 3,513,641 to 3,513,716
tRNA-Phe-3
SO_t080: tRNA-Thr-5 - tRNA-Thr (RefSeq), at 3,513,738 to 3,513,813
tRNA-Thr-5
Position (kb)
3512
3513
3514 Strain fitness (log2 ratio)
-2
-1
0
1 at 3511.882 kb on - strand, within SO3369 at 3511.904 kb on + strand, within SO3369 at 3511.992 kb on + strand, within SO3369 at 3512.048 kb on + strand at 3512.056 kb on - strand at 3512.056 kb on - strand at 3512.157 kb on - strand at 3512.163 kb on + strand at 3512.163 kb on + strand at 3512.170 kb on + strand at 3512.249 kb on + strand, within SO3370 at 3512.257 kb on - strand, within SO3370 at 3512.263 kb on + strand, within SO3370 at 3512.402 kb on + strand, within SO3370 at 3512.428 kb on - strand, within SO3370 at 3512.495 kb on + strand, within SO3370 at 3512.495 kb on + strand, within SO3370 at 3512.509 kb on - strand, within SO3370 at 3512.509 kb on - strand, within SO3370 at 3512.560 kb on + strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.587 kb on + strand, within SO3370 at 3512.671 kb on - strand at 3512.732 kb on - strand at 3512.734 kb on + strand at 3512.734 kb on + strand at 3512.742 kb on - strand at 3512.786 kb on - strand, within SO3371 at 3512.804 kb on + strand, within SO3371 at 3512.804 kb on + strand, within SO3371 at 3512.825 kb on + strand, within SO3371 at 3512.871 kb on - strand, within SO3371 at 3512.904 kb on - strand, within SO3371 at 3512.986 kb on - strand, within SO3371 at 3513.038 kb on - strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.117 kb on + strand, within SO3371 at 3513.120 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.125 kb on - strand, within SO3371 at 3513.182 kb on + strand, within SO3371 at 3513.192 kb on + strand, within SO3371 at 3513.195 kb on - strand, within SO3371 at 3513.268 kb on + strand at 3513.276 kb on - strand at 3513.396 kb on + strand at 3513.404 kb on - strand at 3513.449 kb on - strand at 3513.449 kb on - strand at 3513.551 kb on + strand at 3513.594 kb on + strand at 3513.890 kb on + strand at 3513.931 kb on + strand at 3513.932 kb on - strand at 3513.955 kb on - strand at 3514.031 kb on - strand at 3514.120 kb on - strand at 3514.142 kb on - strand at 3514.235 kb on + strand at 3514.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 3,511,882 - SO3369 0.43 -1.2 3,511,904 + SO3369 0.51 -0.9 3,511,992 + SO3369 0.82 -0.4 3,512,048 + +0.1 3,512,056 - +0.2 3,512,056 - -2.2 3,512,157 - -0.4 3,512,163 + +0.0 3,512,163 + -0.8 3,512,170 + +0.3 3,512,249 + SO3370 0.22 +0.4 3,512,257 - SO3370 0.23 +0.0 3,512,263 + SO3370 0.24 -0.4 3,512,402 + SO3370 0.49 -0.1 3,512,428 - SO3370 0.53 -0.9 3,512,495 + SO3370 0.65 -1.2 3,512,495 + SO3370 0.65 -0.4 3,512,509 - SO3370 0.67 +0.5 3,512,509 - SO3370 0.67 +0.3 3,512,560 + SO3370 0.76 -0.4 3,512,568 - SO3370 0.77 -0.9 3,512,568 - SO3370 0.77 -0.4 3,512,568 - SO3370 0.77 +0.3 3,512,568 - SO3370 0.77 -1.0 3,512,568 - SO3370 0.77 -1.0 3,512,587 + SO3370 0.81 +0.7 3,512,671 - +0.1 3,512,732 - -0.9 3,512,734 + +0.1 3,512,734 + +0.2 3,512,742 - +0.7 3,512,786 - SO3371 0.15 +0.3 3,512,804 + SO3371 0.18 -0.2 3,512,804 + SO3371 0.18 +0.7 3,512,825 + SO3371 0.22 +0.9 3,512,871 - SO3371 0.31 +0.2 3,512,904 - SO3371 0.37 +0.6 3,512,986 - SO3371 0.51 +1.0 3,513,038 - SO3371 0.61 +0.8 3,513,117 + SO3371 0.75 -1.1 3,513,117 + SO3371 0.75 +0.8 3,513,117 + SO3371 0.75 +0.4 3,513,117 + SO3371 0.75 -0.1 3,513,117 + SO3371 0.75 -0.6 3,513,120 - SO3371 0.76 +0.5 3,513,125 - SO3371 0.77 -0.0 3,513,125 - SO3371 0.77 -0.6 3,513,125 - SO3371 0.77 +0.5 3,513,125 - SO3371 0.77 +0.0 3,513,182 + SO3371 0.87 +0.8 3,513,192 + SO3371 0.89 +1.1 3,513,195 - SO3371 0.89 +0.4 3,513,268 + -0.2 3,513,276 - +0.4 3,513,396 + +0.5 3,513,404 - +0.3 3,513,449 - -0.4 3,513,449 - -0.5 3,513,551 + +1.1 3,513,594 + +0.7 3,513,890 + +0.2 3,513,931 + -0.7 3,513,932 - -0.7 3,513,955 - -0.6 3,514,031 - -0.4 3,514,120 - -0.2 3,514,142 - -0.0 3,514,235 + -0.2 3,514,243 - +0.1
Or see this region's nucleotide sequence