Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvB and SO3263 overlap by 41 nucleotides SO3263 and SO3264 are separated by 19 nucleotides SO3264 and SO3265 overlap by 4 nucleotides
SO3262: ilvB - acetolactate synthase isozyme I, large subunit (NCBI ptt file), at 3,397,800 to 3,399,545
ilvB
SO3263: SO3263 - 3-oxoacyl-(acyl-carrier-protein) reductase, putative (NCBI ptt file), at 3,399,505 to 3,400,272
SO3263
SO3264: SO3264 - hypothetical protein (NCBI ptt file), at 3,400,292 to 3,401,479
SO3264
SO3265: SO3265 - conserved hypothetical protein (NCBI ptt file), at 3,401,476 to 3,402,714
SO3265
Position (kb)
3400
3401
3402 Strain fitness (log2 ratio)
-1
0
1
2 at 3399.341 kb on - strand, within ilvB at 3399.368 kb on + strand, within ilvB at 3399.454 kb on - strand at 3399.454 kb on - strand at 3399.499 kb on - strand at 3399.517 kb on - strand at 3399.532 kb on + strand at 3399.592 kb on + strand, within SO3263 at 3399.713 kb on - strand, within SO3263 at 3399.719 kb on - strand, within SO3263 at 3399.725 kb on + strand, within SO3263 at 3399.725 kb on + strand, within SO3263 at 3399.733 kb on - strand, within SO3263 at 3399.852 kb on + strand, within SO3263 at 3399.852 kb on + strand, within SO3263 at 3399.852 kb on + strand, within SO3263 at 3399.857 kb on + strand, within SO3263 at 3399.876 kb on + strand, within SO3263 at 3399.880 kb on - strand, within SO3263 at 3399.880 kb on - strand, within SO3263 at 3399.880 kb on - strand, within SO3263 at 3400.171 kb on + strand, within SO3263 at 3400.171 kb on + strand, within SO3263 at 3400.217 kb on + strand at 3400.346 kb on + strand at 3400.346 kb on + strand at 3400.354 kb on - strand at 3400.472 kb on + strand, within SO3264 at 3400.578 kb on + strand, within SO3264 at 3400.588 kb on - strand, within SO3264 at 3400.604 kb on - strand, within SO3264 at 3400.613 kb on - strand, within SO3264 at 3400.732 kb on + strand, within SO3264 at 3400.757 kb on - strand, within SO3264 at 3400.778 kb on - strand, within SO3264 at 3400.807 kb on - strand, within SO3264 at 3400.876 kb on - strand, within SO3264 at 3400.935 kb on - strand, within SO3264 at 3400.938 kb on + strand, within SO3264 at 3400.956 kb on + strand, within SO3264 at 3401.003 kb on + strand, within SO3264 at 3401.066 kb on - strand, within SO3264 at 3401.066 kb on - strand, within SO3264 at 3401.154 kb on + strand, within SO3264 at 3401.154 kb on + strand, within SO3264 at 3401.162 kb on - strand, within SO3264 at 3401.162 kb on - strand, within SO3264 at 3401.162 kb on - strand, within SO3264 at 3401.191 kb on + strand, within SO3264 at 3401.227 kb on + strand, within SO3264 at 3401.237 kb on + strand, within SO3264 at 3401.275 kb on + strand, within SO3264 at 3401.295 kb on + strand, within SO3264 at 3401.295 kb on + strand, within SO3264 at 3401.302 kb on - strand, within SO3264 at 3401.303 kb on - strand, within SO3264 at 3401.303 kb on - strand, within SO3264 at 3401.348 kb on + strand, within SO3264 at 3401.356 kb on - strand, within SO3264 at 3401.426 kb on + strand at 3401.434 kb on - strand at 3401.436 kb on + strand at 3401.436 kb on + strand at 3401.444 kb on - strand at 3401.524 kb on - strand at 3401.593 kb on + strand at 3401.602 kb on - strand, within SO3265 at 3401.604 kb on + strand, within SO3265 at 3401.723 kb on - strand, within SO3265 at 3401.725 kb on - strand, within SO3265 at 3401.863 kb on + strand, within SO3265 at 3401.876 kb on + strand, within SO3265 at 3401.884 kb on - strand, within SO3265 at 3401.975 kb on + strand, within SO3265 at 3402.023 kb on + strand, within SO3265 at 3402.023 kb on + strand, within SO3265 at 3402.023 kb on + strand, within SO3265 at 3402.031 kb on - strand, within SO3265 at 3402.031 kb on - strand, within SO3265 at 3402.031 kb on - strand, within SO3265 at 3402.096 kb on + strand, within SO3265 at 3402.206 kb on + strand, within SO3265 at 3402.277 kb on + strand, within SO3265 at 3402.290 kb on - strand, within SO3265 at 3402.290 kb on - strand, within SO3265 at 3402.295 kb on - strand, within SO3265 at 3402.373 kb on + strand, within SO3265 at 3402.373 kb on + strand, within SO3265 at 3402.420 kb on + strand, within SO3265 at 3402.470 kb on - strand, within SO3265 at 3402.470 kb on - strand, within SO3265 at 3402.470 kb on - strand, within SO3265 at 3402.478 kb on + strand, within SO3265
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 3,399,341 - ilvB SO3262 0.88 +0.9 3,399,368 + ilvB SO3262 0.90 +2.0 3,399,454 - +2.0 3,399,454 - +0.1 3,399,499 - +2.1 3,399,517 - +0.8 3,399,532 + +0.9 3,399,592 + SO3263 0.11 +1.5 3,399,713 - SO3263 0.27 +1.2 3,399,719 - SO3263 0.28 +2.0 3,399,725 + SO3263 0.29 +2.0 3,399,725 + SO3263 0.29 +0.8 3,399,733 - SO3263 0.30 +2.1 3,399,852 + SO3263 0.45 +0.9 3,399,852 + SO3263 0.45 +1.5 3,399,852 + SO3263 0.45 +1.9 3,399,857 + SO3263 0.46 +1.5 3,399,876 + SO3263 0.48 +1.3 3,399,880 - SO3263 0.49 +1.5 3,399,880 - SO3263 0.49 +2.0 3,399,880 - SO3263 0.49 +1.7 3,400,171 + SO3263 0.87 +2.7 3,400,171 + SO3263 0.87 +1.8 3,400,217 + +1.8 3,400,346 + -0.3 3,400,346 + +0.4 3,400,354 - +0.2 3,400,472 + SO3264 0.15 -0.8 3,400,578 + SO3264 0.24 +0.0 3,400,588 - SO3264 0.25 +1.5 3,400,604 - SO3264 0.26 +0.9 3,400,613 - SO3264 0.27 +1.3 3,400,732 + SO3264 0.37 -0.9 3,400,757 - SO3264 0.39 +1.5 3,400,778 - SO3264 0.41 +1.6 3,400,807 - SO3264 0.43 +0.3 3,400,876 - SO3264 0.49 +1.3 3,400,935 - SO3264 0.54 +1.8 3,400,938 + SO3264 0.54 +0.8 3,400,956 + SO3264 0.56 -0.6 3,401,003 + SO3264 0.60 -0.0 3,401,066 - SO3264 0.65 -0.1 3,401,066 - SO3264 0.65 -1.0 3,401,154 + SO3264 0.73 -0.5 3,401,154 + SO3264 0.73 +0.2 3,401,162 - SO3264 0.73 -0.4 3,401,162 - SO3264 0.73 +2.1 3,401,162 - SO3264 0.73 -0.9 3,401,191 + SO3264 0.76 +0.1 3,401,227 + SO3264 0.79 +0.0 3,401,237 + SO3264 0.80 +0.1 3,401,275 + SO3264 0.83 +0.1 3,401,295 + SO3264 0.84 -0.5 3,401,295 + SO3264 0.84 +0.8 3,401,302 - SO3264 0.85 +1.5 3,401,303 - SO3264 0.85 +2.2 3,401,303 - SO3264 0.85 +1.6 3,401,348 + SO3264 0.89 -0.3 3,401,356 - SO3264 0.90 +2.1 3,401,426 + -0.2 3,401,434 - +1.8 3,401,436 + -0.6 3,401,436 + +0.8 3,401,444 - +2.3 3,401,524 - +2.1 3,401,593 + +1.4 3,401,602 - SO3265 0.10 +1.3 3,401,604 + SO3265 0.10 +1.7 3,401,723 - SO3265 0.20 +0.2 3,401,725 - SO3265 0.20 +0.6 3,401,863 + SO3265 0.31 +0.5 3,401,876 + SO3265 0.32 +1.6 3,401,884 - SO3265 0.33 +1.9 3,401,975 + SO3265 0.40 +2.7 3,402,023 + SO3265 0.44 +0.7 3,402,023 + SO3265 0.44 +2.4 3,402,023 + SO3265 0.44 +1.2 3,402,031 - SO3265 0.45 +1.7 3,402,031 - SO3265 0.45 +1.6 3,402,031 - SO3265 0.45 +0.8 3,402,096 + SO3265 0.50 +0.7 3,402,206 + SO3265 0.59 +2.2 3,402,277 + SO3265 0.65 +2.2 3,402,290 - SO3265 0.66 +2.1 3,402,290 - SO3265 0.66 +1.1 3,402,295 - SO3265 0.66 +1.7 3,402,373 + SO3265 0.72 +2.2 3,402,373 + SO3265 0.72 +1.2 3,402,420 + SO3265 0.76 +2.6 3,402,470 - SO3265 0.80 +1.2 3,402,470 - SO3265 0.80 +1.1 3,402,470 - SO3265 0.80 +1.6 3,402,478 + SO3265 0.81 +2.4
Or see this region's nucleotide sequence