Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2557 and dnaQ-2 are separated by 76 nucleotides dnaQ-2 and rnhA are separated by 70 nucleotides rnhA and SO2561 are separated by 28 nucleotides SO2561 and SO2562 are separated by 23 nucleotides
SO2557: SO2557 - conserved hypothetical protein (NCBI ptt file), at 2,687,860 to 2,689,299
SO2557
SO2559: dnaQ-2 - DNA polymerase III, epsilon subunit (NCBI ptt file), at 2,689,376 to 2,690,104
dnaQ-2
SO2560: rnhA - ribonuclease HI (NCBI ptt file), at 2,690,175 to 2,690,651
rnhA
SO2561: SO2561 - transcriptional regulator, LysR family (NCBI ptt file), at 2,690,680 to 2,691,579
SO2561
SO2562: SO2562 - conserved hypothetical protein (NCBI ptt file), at 2,691,603 to 2,692,337
SO2562
Position (kb)
2690
2691 Strain fitness (log2 ratio)
-1
0
1 at 2689.264 kb on - strand at 2689.305 kb on + strand at 2690.549 kb on - strand, within rnhA at 2690.730 kb on + strand at 2690.802 kb on - strand, within SO2561 at 2690.812 kb on + strand, within SO2561 at 2690.820 kb on - strand, within SO2561 at 2691.002 kb on + strand, within SO2561 at 2691.010 kb on - strand, within SO2561 at 2691.130 kb on - strand, within SO2561 at 2691.389 kb on - strand, within SO2561 at 2691.485 kb on + strand, within SO2561 at 2691.512 kb on + strand at 2691.528 kb on - strand at 2691.530 kb on - strand at 2691.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 2,689,264 - +0.5 2,689,305 + +1.1 2,690,549 - rnhA SO2560 0.78 -1.5 2,690,730 + -0.1 2,690,802 - SO2561 0.14 -0.7 2,690,812 + SO2561 0.15 +0.2 2,690,820 - SO2561 0.16 -0.3 2,691,002 + SO2561 0.36 +0.2 2,691,010 - SO2561 0.37 +0.1 2,691,130 - SO2561 0.50 -0.8 2,691,389 - SO2561 0.79 +0.5 2,691,485 + SO2561 0.89 +0.3 2,691,512 + +0.5 2,691,528 - -0.4 2,691,530 - +0.8 2,691,641 - -1.1
Or see this region's nucleotide sequence