Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt def-3 and SO2532 are separated by 293 nucleotides SO2532 and SO2533 are separated by 129 nucleotides SO2533 and SO2535 are separated by 139 nucleotides
SO2530: def-3 - polypeptide deformylase (NCBI ptt file), at 2,660,149 to 2,660,640
def-3
SO2532: SO2532 - methylated-DNA--protein-cysteine methyltransferase, putative (NCBI ptt file), at 2,660,934 to 2,661,293
SO2532
SO2533: SO2533 - conserved hypothetical protein (NCBI ptt file), at 2,661,423 to 2,662,223
SO2533
SO2535: SO2535 - hypothetical amidotransferase (NCBI ptt file), at 2,662,363 to 2,663,142
SO2535
Position (kb)
2661
2662
2663 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2660.477 kb on + strand, within def-3 at 2660.477 kb on + strand, within def-3 at 2660.532 kb on - strand, within def-3 at 2660.532 kb on - strand, within def-3 at 2660.581 kb on + strand, within def-3 at 2660.680 kb on + strand at 2660.817 kb on - strand at 2660.933 kb on - strand at 2660.968 kb on + strand at 2661.004 kb on - strand, within SO2532 at 2661.123 kb on - strand, within SO2532 at 2661.317 kb on + strand at 2661.381 kb on - strand at 2661.406 kb on + strand at 2661.406 kb on + strand at 2661.414 kb on - strand at 2661.451 kb on + strand at 2661.451 kb on + strand at 2661.654 kb on - strand, within SO2533 at 2661.683 kb on + strand, within SO2533 at 2661.691 kb on - strand, within SO2533 at 2661.696 kb on + strand, within SO2533 at 2661.766 kb on - strand, within SO2533 at 2661.766 kb on - strand, within SO2533 at 2661.982 kb on - strand, within SO2533 at 2662.085 kb on + strand, within SO2533 at 2662.097 kb on + strand, within SO2533 at 2662.130 kb on + strand, within SO2533 at 2662.253 kb on - strand at 2662.545 kb on - strand, within SO2535 at 2662.569 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.626 kb on + strand, within SO2535 at 2662.634 kb on - strand, within SO2535 at 2662.634 kb on - strand, within SO2535 at 2662.634 kb on - strand, within SO2535 at 2662.738 kb on - strand, within SO2535 at 2662.761 kb on + strand, within SO2535 at 2662.818 kb on - strand, within SO2535 at 2662.949 kb on - strand, within SO2535 at 2662.954 kb on - strand, within SO2535 at 2663.008 kb on + strand, within SO2535 at 2663.016 kb on - strand, within SO2535 at 2663.025 kb on + strand, within SO2535 at 2663.073 kb on + strand at 2663.073 kb on + strand at 2663.081 kb on - strand at 2663.081 kb on - strand at 2663.082 kb on - strand at 2663.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 2,660,477 + def-3 SO2530 0.67 +0.2 2,660,477 + def-3 SO2530 0.67 -0.3 2,660,532 - def-3 SO2530 0.78 -0.2 2,660,532 - def-3 SO2530 0.78 +0.2 2,660,581 + def-3 SO2530 0.88 -0.9 2,660,680 + -0.0 2,660,817 - +0.3 2,660,933 - +0.5 2,660,968 + -0.5 2,661,004 - SO2532 0.19 -2.4 2,661,123 - SO2532 0.53 +0.3 2,661,317 + +0.4 2,661,381 - -2.4 2,661,406 + -0.2 2,661,406 + -0.6 2,661,414 - -2.4 2,661,451 + -0.9 2,661,451 + -3.1 2,661,654 - SO2533 0.29 -3.6 2,661,683 + SO2533 0.32 -0.9 2,661,691 - SO2533 0.33 -1.0 2,661,696 + SO2533 0.34 -1.6 2,661,766 - SO2533 0.43 -2.9 2,661,766 - SO2533 0.43 -1.1 2,661,982 - SO2533 0.70 -1.0 2,662,085 + SO2533 0.83 -1.5 2,662,097 + SO2533 0.84 -1.3 2,662,130 + SO2533 0.88 -1.4 2,662,253 - +0.3 2,662,545 - SO2535 0.23 -0.9 2,662,569 + SO2535 0.26 +0.3 2,662,626 + SO2535 0.34 -1.2 2,662,626 + SO2535 0.34 -1.0 2,662,626 + SO2535 0.34 +0.1 2,662,626 + SO2535 0.34 +1.1 2,662,634 - SO2535 0.35 +0.6 2,662,634 - SO2535 0.35 +0.6 2,662,634 - SO2535 0.35 +0.2 2,662,738 - SO2535 0.48 +0.1 2,662,761 + SO2535 0.51 -0.1 2,662,818 - SO2535 0.58 -0.1 2,662,949 - SO2535 0.75 -0.6 2,662,954 - SO2535 0.76 +0.8 2,663,008 + SO2535 0.83 +0.5 2,663,016 - SO2535 0.84 -0.6 2,663,025 + SO2535 0.85 -1.2 2,663,073 + -0.0 2,663,073 + +0.8 2,663,081 - +0.2 2,663,081 - -1.1 2,663,082 - -0.2 2,663,105 + -0.2
Or see this region's nucleotide sequence