Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdaA and SO2249 overlap by 8 nucleotides SO2249 and nagZ are separated by 41 nucleotides nagZ and SO2251 are separated by 81 nucleotides
SO2248: sdaA - L-serine dehydratase 1 (NCBI ptt file), at 2,362,473 to 2,363,849
sdaA
SO2249: SO2249 - hypothetical protein (NCBI ptt file), at 2,363,842 to 2,364,192
SO2249
SO2250: nagZ - beta-hexosaminidase (NCBI ptt file), at 2,364,234 to 2,365,262
nagZ
SO2251: SO2251 - conserved hypothetical protein (NCBI ptt file), at 2,365,344 to 2,365,883
SO2251
Position (kb)
2364
2365
2366 Strain fitness (log2 ratio)
-1
0
1 at 2363.243 kb on + strand, within sdaA at 2363.373 kb on - strand, within sdaA at 2363.454 kb on - strand, within sdaA at 2363.465 kb on + strand, within sdaA at 2363.681 kb on - strand, within sdaA at 2363.700 kb on + strand, within sdaA at 2363.713 kb on - strand at 2364.270 kb on - strand at 2364.275 kb on - strand at 2364.309 kb on - strand at 2364.972 kb on - strand, within nagZ at 2365.297 kb on + strand at 2365.297 kb on + strand at 2365.527 kb on + strand, within SO2251 at 2365.744 kb on + strand, within SO2251 at 2365.754 kb on + strand, within SO2251 at 2365.875 kb on - strand at 2365.875 kb on - strand at 2365.885 kb on - strand at 2365.909 kb on - strand at 2366.024 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 2,363,243 + sdaA SO2248 0.56 -0.8 2,363,373 - sdaA SO2248 0.65 -0.6 2,363,454 - sdaA SO2248 0.71 -0.9 2,363,465 + sdaA SO2248 0.72 -0.5 2,363,681 - sdaA SO2248 0.88 +0.0 2,363,700 + sdaA SO2248 0.89 +0.5 2,363,713 - -1.7 2,364,270 - +0.6 2,364,275 - +0.4 2,364,309 - +0.3 2,364,972 - nagZ SO2250 0.72 +1.1 2,365,297 + +1.1 2,365,297 + +0.4 2,365,527 + SO2251 0.34 +0.6 2,365,744 + SO2251 0.74 +0.5 2,365,754 + SO2251 0.76 +0.1 2,365,875 - -0.4 2,365,875 - -0.2 2,365,885 - +0.5 2,365,909 - +0.8 2,366,024 + -1.7
Or see this region's nucleotide sequence