Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ggt-2 and SO1953 are separated by 108 nucleotides SO1953 and SO1954 are separated by 77 nucleotides SO1954 and SO1955 are separated by 285 nucleotides
SO1952: ggt-2 - gamma-glutamyltranspeptidase (NCBI ptt file), at 2,057,821 to 2,059,560
ggt-2
SO1953: SO1953 - conserved hypothetical protein (NCBI ptt file), at 2,059,669 to 2,060,967
SO1953
SO1954: SO1954 - transporter, LysE family (NCBI ptt file), at 2,061,045 to 2,061,668
SO1954
SO1955: SO1955 - hypothetical protein (NCBI ptt file), at 2,061,954 to 2,062,406
SO1955
Position (kb)
2059
2060
2061 Strain fitness (log2 ratio)
-2
-1
0
1 at 2058.685 kb on - strand, within ggt-2 at 2058.702 kb on + strand, within ggt-2 at 2058.800 kb on + strand, within ggt-2 at 2058.881 kb on + strand, within ggt-2 at 2058.958 kb on - strand, within ggt-2 at 2059.107 kb on + strand, within ggt-2 at 2059.300 kb on + strand, within ggt-2 at 2059.318 kb on + strand, within ggt-2 at 2059.478 kb on - strand at 2059.543 kb on - strand at 2059.543 kb on - strand at 2059.673 kb on - strand at 2059.781 kb on + strand at 2059.781 kb on + strand at 2059.781 kb on + strand at 2059.789 kb on - strand at 2059.789 kb on - strand at 2059.789 kb on - strand at 2059.976 kb on - strand, within SO1953 at 2060.007 kb on + strand, within SO1953 at 2060.036 kb on + strand, within SO1953 at 2060.044 kb on - strand, within SO1953 at 2060.388 kb on - strand, within SO1953 at 2060.422 kb on + strand, within SO1953 at 2060.565 kb on + strand, within SO1953 at 2060.619 kb on - strand, within SO1953 at 2060.696 kb on + strand, within SO1953 at 2060.816 kb on + strand, within SO1953 at 2060.895 kb on + strand at 2060.941 kb on + strand at 2061.170 kb on + strand, within SO1954 at 2061.170 kb on + strand, within SO1954 at 2061.170 kb on + strand, within SO1954 at 2061.170 kb on + strand, within SO1954 at 2061.170 kb on + strand, within SO1954 at 2061.170 kb on + strand, within SO1954 at 2061.173 kb on - strand, within SO1954 at 2061.178 kb on - strand, within SO1954 at 2061.199 kb on - strand, within SO1954 at 2061.201 kb on + strand, within SO1954 at 2061.209 kb on + strand, within SO1954 at 2061.209 kb on - strand, within SO1954 at 2061.282 kb on + strand, within SO1954 at 2061.283 kb on + strand, within SO1954 at 2061.283 kb on + strand, within SO1954 at 2061.283 kb on + strand, within SO1954 at 2061.283 kb on + strand, within SO1954 at 2061.283 kb on + strand, within SO1954 at 2061.291 kb on - strand, within SO1954 at 2061.291 kb on - strand, within SO1954 at 2061.291 kb on - strand, within SO1954 at 2061.291 kb on - strand, within SO1954 at 2061.408 kb on + strand, within SO1954 at 2061.515 kb on - strand, within SO1954 at 2061.520 kb on + strand, within SO1954 at 2061.520 kb on + strand, within SO1954 at 2061.520 kb on + strand, within SO1954 at 2061.528 kb on - strand, within SO1954 at 2061.528 kb on - strand, within SO1954 at 2061.528 kb on - strand, within SO1954 at 2061.528 kb on - strand, within SO1954 at 2061.528 kb on - strand, within SO1954 at 2061.550 kb on + strand, within SO1954 at 2061.554 kb on + strand, within SO1954 at 2061.558 kb on - strand, within SO1954 at 2061.592 kb on + strand, within SO1954 at 2061.592 kb on + strand, within SO1954 at 2061.600 kb on - strand, within SO1954 at 2061.669 kb on + strand at 2061.677 kb on - strand at 2061.709 kb on - strand at 2061.748 kb on + strand at 2061.748 kb on + strand at 2061.786 kb on + strand at 2061.794 kb on - strand at 2061.804 kb on - strand at 2061.836 kb on + strand at 2061.960 kb on + strand at 2061.960 kb on + strand at 2061.960 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 2,058,685 - ggt-2 SO1952 0.50 +0.6 2,058,702 + ggt-2 SO1952 0.51 -0.1 2,058,800 + ggt-2 SO1952 0.56 +0.1 2,058,881 + ggt-2 SO1952 0.61 -0.8 2,058,958 - ggt-2 SO1952 0.65 -0.4 2,059,107 + ggt-2 SO1952 0.74 -0.3 2,059,300 + ggt-2 SO1952 0.85 -0.4 2,059,318 + ggt-2 SO1952 0.86 -0.5 2,059,478 - -0.2 2,059,543 - +0.2 2,059,543 - +0.6 2,059,673 - -0.1 2,059,781 + +0.6 2,059,781 + -0.5 2,059,781 + +1.3 2,059,789 - +0.5 2,059,789 - -2.6 2,059,789 - +1.3 2,059,976 - SO1953 0.24 -0.3 2,060,007 + SO1953 0.26 +1.5 2,060,036 + SO1953 0.28 +0.4 2,060,044 - SO1953 0.29 +1.4 2,060,388 - SO1953 0.55 +0.2 2,060,422 + SO1953 0.58 +0.4 2,060,565 + SO1953 0.69 -0.4 2,060,619 - SO1953 0.73 +0.0 2,060,696 + SO1953 0.79 -0.0 2,060,816 + SO1953 0.88 -0.9 2,060,895 + -1.7 2,060,941 + -0.7 2,061,170 + SO1954 0.20 +0.5 2,061,170 + SO1954 0.20 +0.9 2,061,170 + SO1954 0.20 -0.4 2,061,170 + SO1954 0.20 -0.5 2,061,170 + SO1954 0.20 -0.6 2,061,170 + SO1954 0.20 -1.0 2,061,173 - SO1954 0.21 +0.9 2,061,178 - SO1954 0.21 +0.4 2,061,199 - SO1954 0.25 +0.1 2,061,201 + SO1954 0.25 -0.0 2,061,209 + SO1954 0.26 +0.5 2,061,209 - SO1954 0.26 -0.3 2,061,282 + SO1954 0.38 -0.0 2,061,283 + SO1954 0.38 +0.5 2,061,283 + SO1954 0.38 -0.3 2,061,283 + SO1954 0.38 -0.4 2,061,283 + SO1954 0.38 +0.7 2,061,283 + SO1954 0.38 -0.7 2,061,291 - SO1954 0.39 -0.4 2,061,291 - SO1954 0.39 +0.4 2,061,291 - SO1954 0.39 -0.0 2,061,291 - SO1954 0.39 -0.9 2,061,408 + SO1954 0.58 +0.7 2,061,515 - SO1954 0.75 +0.4 2,061,520 + SO1954 0.76 +0.3 2,061,520 + SO1954 0.76 +0.4 2,061,520 + SO1954 0.76 -0.6 2,061,528 - SO1954 0.77 -1.4 2,061,528 - SO1954 0.77 +0.3 2,061,528 - SO1954 0.77 +0.0 2,061,528 - SO1954 0.77 -0.9 2,061,528 - SO1954 0.77 -0.8 2,061,550 + SO1954 0.81 -0.9 2,061,554 + SO1954 0.82 +0.3 2,061,558 - SO1954 0.82 +0.5 2,061,592 + SO1954 0.88 +0.0 2,061,592 + SO1954 0.88 -0.1 2,061,600 - SO1954 0.89 +0.1 2,061,669 + -0.6 2,061,677 - +0.3 2,061,709 - +0.0 2,061,748 + -1.2 2,061,748 + -0.4 2,061,786 + -0.9 2,061,794 - -0.3 2,061,804 - +0.3 2,061,836 + +0.7 2,061,960 + -2.1 2,061,960 + +0.3 2,061,960 + -0.0
Or see this region's nucleotide sequence