Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nosA and hrpB are separated by 208 nucleotides hrpB and mrcB are separated by 77 nucleotides
SO0630: nosA - TonB-dependent receptor (NCBI ptt file), at 655,861 to 657,867
nosA
SO0632: hrpB - ATP-dependent helicase HrpB (NCBI ptt file), at 658,076 to 660,583
hrpB
SO0633: mrcB - penicillin-binding protein 1B (NCBI ptt file), at 660,661 to 662,967
mrcB
Position (kb) 658
659
660
661 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 657.116 kb on - strand, within nosA at 657.119 kb on + strand, within nosA at 657.149 kb on - strand, within nosA at 657.178 kb on + strand, within nosA at 657.178 kb on + strand, within nosA at 657.250 kb on - strand, within nosA at 657.324 kb on + strand, within nosA at 657.324 kb on - strand, within nosA at 657.398 kb on - strand, within nosA at 657.421 kb on + strand, within nosA at 657.421 kb on + strand, within nosA at 657.421 kb on + strand, within nosA at 657.421 kb on + strand, within nosA at 657.422 kb on + strand, within nosA at 657.422 kb on + strand, within nosA at 657.429 kb on - strand, within nosA at 657.429 kb on - strand, within nosA at 657.449 kb on + strand, within nosA at 657.569 kb on - strand, within nosA at 657.760 kb on - strand at 657.779 kb on - strand at 657.810 kb on + strand at 657.861 kb on + strand at 658.022 kb on + strand at 658.022 kb on + strand at 658.030 kb on - strand at 658.030 kb on - strand at 658.142 kb on - strand at 658.284 kb on + strand at 658.305 kb on + strand at 658.317 kb on + strand at 658.317 kb on + strand at 658.317 kb on + strand at 658.317 kb on + strand at 658.317 kb on + strand at 658.317 kb on + strand at 658.325 kb on - strand at 658.325 kb on - strand at 658.325 kb on - strand at 658.325 kb on - strand at 658.325 kb on - strand at 658.344 kb on - strand, within hrpB at 658.459 kb on + strand, within hrpB at 658.485 kb on + strand, within hrpB at 658.491 kb on + strand, within hrpB at 658.499 kb on + strand, within hrpB at 658.514 kb on - strand, within hrpB at 658.584 kb on + strand, within hrpB at 658.584 kb on + strand, within hrpB at 658.594 kb on + strand, within hrpB at 658.594 kb on + strand, within hrpB at 658.594 kb on + strand, within hrpB at 658.646 kb on - strand, within hrpB at 658.657 kb on - strand, within hrpB at 658.670 kb on + strand, within hrpB at 658.670 kb on + strand, within hrpB at 658.678 kb on - strand, within hrpB at 658.693 kb on - strand, within hrpB at 658.693 kb on - strand, within hrpB at 658.693 kb on - strand, within hrpB at 658.772 kb on + strand, within hrpB at 658.777 kb on + strand, within hrpB at 658.777 kb on + strand, within hrpB at 658.777 kb on + strand, within hrpB at 658.777 kb on + strand, within hrpB at 658.785 kb on - strand, within hrpB at 658.852 kb on + strand, within hrpB at 658.852 kb on + strand, within hrpB at 658.852 kb on + strand, within hrpB at 658.855 kb on - strand, within hrpB at 658.860 kb on - strand, within hrpB at 658.860 kb on - strand, within hrpB at 658.885 kb on + strand, within hrpB at 658.890 kb on + strand, within hrpB at 658.890 kb on + strand, within hrpB at 658.898 kb on - strand, within hrpB at 658.916 kb on - strand, within hrpB at 658.988 kb on - strand, within hrpB at 659.021 kb on - strand, within hrpB at 659.100 kb on + strand, within hrpB at 659.130 kb on - strand, within hrpB at 659.171 kb on + strand, within hrpB at 659.171 kb on + strand, within hrpB at 659.179 kb on - strand, within hrpB at 659.179 kb on - strand, within hrpB at 659.179 kb on - strand, within hrpB at 659.179 kb on - strand, within hrpB at 659.179 kb on - strand, within hrpB at 659.210 kb on + strand, within hrpB at 659.274 kb on + strand, within hrpB at 659.274 kb on + strand, within hrpB at 659.322 kb on - strand, within hrpB at 659.325 kb on + strand, within hrpB at 659.338 kb on - strand, within hrpB at 659.340 kb on + strand, within hrpB at 659.343 kb on - strand, within hrpB at 659.399 kb on + strand, within hrpB at 659.407 kb on - strand, within hrpB at 659.407 kb on - strand, within hrpB at 659.486 kb on + strand, within hrpB at 659.489 kb on - strand, within hrpB at 659.494 kb on - strand, within hrpB at 659.569 kb on + strand, within hrpB at 659.569 kb on - strand, within hrpB at 659.570 kb on + strand, within hrpB at 659.611 kb on - strand, within hrpB at 659.611 kb on - strand, within hrpB at 659.620 kb on + strand, within hrpB at 659.620 kb on + strand, within hrpB at 659.628 kb on - strand, within hrpB at 659.628 kb on - strand, within hrpB at 659.628 kb on - strand, within hrpB at 659.647 kb on - strand, within hrpB at 659.697 kb on + strand, within hrpB at 659.705 kb on - strand, within hrpB at 659.729 kb on - strand, within hrpB at 659.776 kb on + strand, within hrpB at 659.841 kb on + strand, within hrpB at 659.841 kb on + strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.851 kb on - strand, within hrpB at 659.859 kb on - strand, within hrpB at 660.060 kb on + strand, within hrpB at 660.068 kb on - strand, within hrpB at 660.068 kb on - strand, within hrpB at 660.104 kb on - strand, within hrpB at 660.249 kb on + strand, within hrpB at 660.249 kb on - strand, within hrpB at 660.273 kb on + strand, within hrpB at 660.281 kb on - strand, within hrpB at 660.289 kb on + strand, within hrpB at 660.336 kb on + strand at 660.336 kb on + strand at 660.376 kb on + strand at 660.383 kb on - strand at 660.395 kb on - strand at 660.402 kb on + strand at 660.405 kb on - strand at 660.457 kb on + strand at 660.495 kb on + strand at 660.533 kb on - strand at 660.584 kb on + strand at 660.584 kb on + strand at 660.584 kb on + strand at 660.592 kb on - strand at 660.592 kb on - strand at 660.592 kb on - strand at 660.619 kb on + strand at 660.627 kb on - strand at 660.746 kb on + strand at 660.746 kb on + strand at 660.754 kb on - strand at 660.793 kb on + strand at 660.799 kb on - strand at 660.884 kb on - strand at 660.917 kb on - strand, within mrcB at 660.972 kb on - strand, within mrcB at 661.037 kb on + strand, within mrcB at 661.078 kb on - strand, within mrcB at 661.274 kb on - strand, within mrcB at 661.324 kb on + strand, within mrcB at 661.332 kb on - strand, within mrcB at 661.334 kb on + strand, within mrcB at 661.342 kb on - strand, within mrcB at 661.488 kb on - strand, within mrcB
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 657,116 - nosA SO0630 0.63 -0.6 657,119 + nosA SO0630 0.63 -1.0 657,149 - nosA SO0630 0.64 -0.6 657,178 + nosA SO0630 0.66 +0.3 657,178 + nosA SO0630 0.66 +0.4 657,250 - nosA SO0630 0.69 +1.2 657,324 + nosA SO0630 0.73 -0.6 657,324 - nosA SO0630 0.73 +0.6 657,398 - nosA SO0630 0.77 +0.9 657,421 + nosA SO0630 0.78 -0.1 657,421 + nosA SO0630 0.78 -0.4 657,421 + nosA SO0630 0.78 -0.1 657,421 + nosA SO0630 0.78 +0.5 657,422 + nosA SO0630 0.78 -0.0 657,422 + nosA SO0630 0.78 -0.2 657,429 - nosA SO0630 0.78 -0.9 657,429 - nosA SO0630 0.78 +0.3 657,449 + nosA SO0630 0.79 +0.5 657,569 - nosA SO0630 0.85 -0.0 657,760 - +0.1 657,779 - -0.4 657,810 + -0.2 657,861 + +0.2 658,022 + -0.3 658,022 + +0.3 658,030 - +0.6 658,030 - +1.3 658,142 - -1.8 658,284 + +0.2 658,305 + -0.1 658,317 + +0.7 658,317 + +0.2 658,317 + +0.9 658,317 + -1.2 658,317 + +0.3 658,317 + -0.1 658,325 - -0.5 658,325 - -1.0 658,325 - -0.1 658,325 - +0.2 658,325 - +0.1 658,344 - hrpB SO0632 0.11 -0.9 658,459 + hrpB SO0632 0.15 -0.8 658,485 + hrpB SO0632 0.16 +0.1 658,491 + hrpB SO0632 0.17 -3.2 658,499 + hrpB SO0632 0.17 -0.1 658,514 - hrpB SO0632 0.17 -0.0 658,584 + hrpB SO0632 0.20 -0.5 658,584 + hrpB SO0632 0.20 +0.5 658,594 + hrpB SO0632 0.21 -2.3 658,594 + hrpB SO0632 0.21 -0.5 658,594 + hrpB SO0632 0.21 +0.3 658,646 - hrpB SO0632 0.23 +0.0 658,657 - hrpB SO0632 0.23 -0.2 658,670 + hrpB SO0632 0.24 +0.4 658,670 + hrpB SO0632 0.24 +0.7 658,678 - hrpB SO0632 0.24 -0.3 658,693 - hrpB SO0632 0.25 +0.1 658,693 - hrpB SO0632 0.25 +0.2 658,693 - hrpB SO0632 0.25 -2.3 658,772 + hrpB SO0632 0.28 +0.2 658,777 + hrpB SO0632 0.28 -0.9 658,777 + hrpB SO0632 0.28 -1.5 658,777 + hrpB SO0632 0.28 +0.1 658,777 + hrpB SO0632 0.28 +0.5 658,785 - hrpB SO0632 0.28 -0.9 658,852 + hrpB SO0632 0.31 -0.3 658,852 + hrpB SO0632 0.31 +0.5 658,852 + hrpB SO0632 0.31 -0.5 658,855 - hrpB SO0632 0.31 -0.1 658,860 - hrpB SO0632 0.31 -3.8 658,860 - hrpB SO0632 0.31 +0.4 658,885 + hrpB SO0632 0.32 -0.4 658,890 + hrpB SO0632 0.32 -1.3 658,890 + hrpB SO0632 0.32 -0.1 658,898 - hrpB SO0632 0.33 +0.2 658,916 - hrpB SO0632 0.33 -0.6 658,988 - hrpB SO0632 0.36 -0.1 659,021 - hrpB SO0632 0.38 +0.9 659,100 + hrpB SO0632 0.41 -0.3 659,130 - hrpB SO0632 0.42 -0.8 659,171 + hrpB SO0632 0.44 -0.1 659,171 + hrpB SO0632 0.44 -0.4 659,179 - hrpB SO0632 0.44 +0.0 659,179 - hrpB SO0632 0.44 -1.8 659,179 - hrpB SO0632 0.44 -1.4 659,179 - hrpB SO0632 0.44 +0.3 659,179 - hrpB SO0632 0.44 +1.6 659,210 + hrpB SO0632 0.45 -0.5 659,274 + hrpB SO0632 0.48 +0.1 659,274 + hrpB SO0632 0.48 -0.4 659,322 - hrpB SO0632 0.50 -0.1 659,325 + hrpB SO0632 0.50 -0.6 659,338 - hrpB SO0632 0.50 -2.4 659,340 + hrpB SO0632 0.50 -0.0 659,343 - hrpB SO0632 0.51 +0.5 659,399 + hrpB SO0632 0.53 +0.2 659,407 - hrpB SO0632 0.53 -0.4 659,407 - hrpB SO0632 0.53 -0.9 659,486 + hrpB SO0632 0.56 -2.2 659,489 - hrpB SO0632 0.56 -1.4 659,494 - hrpB SO0632 0.57 -1.3 659,569 + hrpB SO0632 0.60 +0.5 659,569 - hrpB SO0632 0.60 -0.1 659,570 + hrpB SO0632 0.60 -0.3 659,611 - hrpB SO0632 0.61 -0.4 659,611 - hrpB SO0632 0.61 +0.3 659,620 + hrpB SO0632 0.62 +0.0 659,620 + hrpB SO0632 0.62 +0.6 659,628 - hrpB SO0632 0.62 -0.0 659,628 - hrpB SO0632 0.62 -0.5 659,628 - hrpB SO0632 0.62 -0.8 659,647 - hrpB SO0632 0.63 -1.5 659,697 + hrpB SO0632 0.65 +0.9 659,705 - hrpB SO0632 0.65 -1.7 659,729 - hrpB SO0632 0.66 -0.8 659,776 + hrpB SO0632 0.68 -0.2 659,841 + hrpB SO0632 0.70 +0.6 659,841 + hrpB SO0632 0.70 -0.1 659,849 - hrpB SO0632 0.71 -0.0 659,849 - hrpB SO0632 0.71 -0.4 659,849 - hrpB SO0632 0.71 -0.3 659,851 - hrpB SO0632 0.71 -0.6 659,859 - hrpB SO0632 0.71 -1.7 660,060 + hrpB SO0632 0.79 -0.5 660,068 - hrpB SO0632 0.79 -0.1 660,068 - hrpB SO0632 0.79 -1.0 660,104 - hrpB SO0632 0.81 -0.3 660,249 + hrpB SO0632 0.87 -0.4 660,249 - hrpB SO0632 0.87 -0.4 660,273 + hrpB SO0632 0.88 -1.1 660,281 - hrpB SO0632 0.88 +0.2 660,289 + hrpB SO0632 0.88 -0.1 660,336 + +1.2 660,336 + +0.4 660,376 + -1.1 660,383 - +0.9 660,395 - -0.9 660,402 + +0.2 660,405 - -0.5 660,457 + -0.1 660,495 + -0.4 660,533 - -1.5 660,584 + +0.6 660,584 + +0.2 660,584 + -0.2 660,592 - -0.7 660,592 - -1.3 660,592 - -1.5 660,619 + +0.2 660,627 - -0.8 660,746 + -3.8 660,746 + -2.1 660,754 - -2.3 660,793 + -0.6 660,799 - -2.8 660,884 - -1.1 660,917 - mrcB SO0633 0.11 -3.0 660,972 - mrcB SO0633 0.13 -1.0 661,037 + mrcB SO0633 0.16 -1.1 661,078 - mrcB SO0633 0.18 -0.5 661,274 - mrcB SO0633 0.27 -2.2 661,324 + mrcB SO0633 0.29 +0.5 661,332 - mrcB SO0633 0.29 -0.1 661,334 + mrcB SO0633 0.29 -2.5 661,342 - mrcB SO0633 0.30 -0.6 661,488 - mrcB SO0633 0.36 -1.1
Or see this region's nucleotide sequence