Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0189 and SO0190 are separated by 237 nucleotides SO0190 and cysQ-1 are separated by 69 nucleotides cysQ-1 and SO0192 are separated by 7 nucleotides
SO0189: SO0189 - fibronectin type III domain protein (NCBI ptt file), at 192,488 to 200,056
SO0189
SO0190: SO0190 - MutT/nudix family protein (NCBI ptt file), at 200,294 to 200,845
SO0190
SO0191: cysQ-1 - cysQ protein (NCBI ptt file), at 200,915 to 201,727
cysQ-1
SO0192: SO0192 - acetyltransferase, GNAT family (NCBI ptt file), at 201,735 to 202,178
SO0192
Position (kb) 200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 199.363 kb on + strand at 199.400 kb on - strand at 199.491 kb on + strand at 199.508 kb on + strand at 199.524 kb on - strand at 199.551 kb on - strand at 199.618 kb on + strand at 199.626 kb on + strand at 199.681 kb on - strand at 199.694 kb on + strand at 199.694 kb on + strand at 199.702 kb on - strand at 199.709 kb on + strand at 199.726 kb on + strand at 199.731 kb on + strand at 199.734 kb on - strand at 199.744 kb on - strand at 199.819 kb on + strand at 199.918 kb on - strand at 199.925 kb on + strand at 200.025 kb on + strand at 200.033 kb on - strand at 200.058 kb on - strand at 200.058 kb on - strand at 200.125 kb on - strand at 200.131 kb on + strand at 200.199 kb on - strand at 200.416 kb on + strand, within SO0190 at 200.610 kb on + strand, within SO0190 at 200.737 kb on + strand, within SO0190 at 200.743 kb on + strand, within SO0190 at 200.751 kb on - strand, within SO0190 at 200.965 kb on + strand at 200.989 kb on + strand at 201.040 kb on - strand, within cysQ-1 at 201.194 kb on + strand, within cysQ-1 at 201.241 kb on + strand, within cysQ-1 at 201.292 kb on + strand, within cysQ-1 at 201.371 kb on + strand, within cysQ-1 at 201.379 kb on - strand, within cysQ-1 at 201.392 kb on + strand, within cysQ-1 at 201.399 kb on + strand, within cysQ-1 at 201.400 kb on - strand, within cysQ-1 at 201.454 kb on + strand, within cysQ-1 at 201.499 kb on - strand, within cysQ-1 at 201.499 kb on - strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.629 kb on + strand, within cysQ-1 at 201.696 kb on - strand at 201.789 kb on + strand, within SO0192 at 201.820 kb on - strand, within SO0192
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 199,363 + +2.1 199,400 - -0.1 199,491 + -1.0 199,508 + -0.1 199,524 - -1.3 199,551 - +1.2 199,618 + +1.3 199,626 + +0.1 199,681 - +0.9 199,694 + +0.5 199,694 + +0.2 199,702 - -2.6 199,709 + -1.1 199,726 + -0.3 199,731 + -0.5 199,734 - -0.5 199,744 - -3.5 199,819 + -1.1 199,918 - -0.1 199,925 + -0.7 200,025 + -0.6 200,033 - +0.3 200,058 - -1.2 200,058 - -0.3 200,125 - +0.7 200,131 + -1.2 200,199 - -0.2 200,416 + SO0190 0.22 -0.5 200,610 + SO0190 0.57 -1.5 200,737 + SO0190 0.80 -1.0 200,743 + SO0190 0.81 -0.5 200,751 - SO0190 0.83 -1.1 200,965 + +0.8 200,989 + -0.2 201,040 - cysQ-1 SO0191 0.15 +1.0 201,194 + cysQ-1 SO0191 0.34 +0.3 201,241 + cysQ-1 SO0191 0.40 -0.4 201,292 + cysQ-1 SO0191 0.46 +0.6 201,371 + cysQ-1 SO0191 0.56 -1.1 201,379 - cysQ-1 SO0191 0.57 -0.0 201,392 + cysQ-1 SO0191 0.59 +0.2 201,399 + cysQ-1 SO0191 0.60 +0.6 201,400 - cysQ-1 SO0191 0.60 -1.2 201,454 + cysQ-1 SO0191 0.66 -1.0 201,499 - cysQ-1 SO0191 0.72 -0.1 201,499 - cysQ-1 SO0191 0.72 +2.1 201,512 + cysQ-1 SO0191 0.73 +0.2 201,512 + cysQ-1 SO0191 0.73 +1.5 201,512 + cysQ-1 SO0191 0.73 +0.6 201,512 + cysQ-1 SO0191 0.73 -0.8 201,512 + cysQ-1 SO0191 0.73 +0.9 201,629 + cysQ-1 SO0191 0.88 +0.3 201,696 - +0.2 201,789 + SO0192 0.12 +0.3 201,820 - SO0192 0.19 +0.7
Or see this region's nucleotide sequence