Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdnG and fdnH are separated by 2 nucleotides fdnH and fdnI overlap by 4 nucleotides fdnI and fdhE overlap by 1 nucleotides
SO0101: fdnG - selenium-containing formate dehydrogenase, nitrate inducible, alpha subunit (NCBI ptt file), at 108,703 to 111,717
fdnG
SO0102: fdnH - formate dehydrogenase, nitrate-inducible, iron-sulfur subunit (NCBI ptt file), at 111,720 to 112,634
fdnH
SO0103: fdnI - formate dehydrogenase, nitrate-inducible, cytochrome b556 subunit (NCBI ptt file), at 112,631 to 113,272
fdnI
SO0104: fdhE - fdhE protein (NCBI ptt file), at 113,272 to 114,180
fdhE
Position (kb) 111
112
113 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 110.757 kb on + strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.764 kb on - strand, within fdnG at 110.790 kb on - strand, within fdnG at 110.849 kb on + strand, within fdnG at 110.872 kb on - strand, within fdnG at 110.937 kb on + strand, within fdnG at 110.945 kb on - strand, within fdnG at 110.982 kb on - strand, within fdnG at 110.994 kb on + strand, within fdnG at 111.000 kb on - strand, within fdnG at 111.002 kb on - strand, within fdnG at 111.027 kb on + strand, within fdnG at 111.032 kb on + strand, within fdnG at 111.050 kb on - strand, within fdnG at 111.080 kb on + strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.088 kb on - strand, within fdnG at 111.145 kb on - strand, within fdnG at 111.191 kb on - strand, within fdnG at 111.191 kb on - strand, within fdnG at 111.262 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.264 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.269 kb on + strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.272 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.277 kb on - strand, within fdnG at 111.328 kb on + strand, within fdnG at 111.330 kb on - strand, within fdnG at 111.336 kb on - strand, within fdnG at 111.409 kb on + strand, within fdnG at 111.409 kb on + strand, within fdnG at 111.522 kb on + strand at 111.536 kb on + strand at 111.675 kb on + strand at 111.675 kb on - strand at 111.711 kb on + strand at 111.756 kb on + strand at 111.764 kb on - strand at 111.787 kb on - strand at 111.823 kb on + strand, within fdnH at 111.841 kb on + strand, within fdnH at 111.871 kb on + strand, within fdnH at 111.871 kb on + strand, within fdnH at 111.871 kb on + strand, within fdnH at 111.941 kb on + strand, within fdnH at 112.014 kb on + strand, within fdnH at 112.022 kb on - strand, within fdnH at 112.022 kb on - strand, within fdnH at 112.163 kb on + strand, within fdnH at 112.185 kb on + strand, within fdnH at 112.212 kb on - strand, within fdnH at 112.247 kb on + strand, within fdnH at 112.247 kb on + strand, within fdnH at 112.250 kb on - strand, within fdnH at 112.293 kb on + strand, within fdnH at 112.355 kb on + strand, within fdnH at 112.355 kb on + strand, within fdnH at 112.368 kb on + strand, within fdnH at 112.368 kb on + strand, within fdnH at 112.375 kb on - strand, within fdnH at 112.423 kb on - strand, within fdnH at 112.423 kb on - strand, within fdnH at 112.467 kb on - strand, within fdnH at 112.521 kb on + strand, within fdnH at 112.597 kb on + strand at 112.598 kb on + strand at 112.665 kb on + strand at 112.665 kb on + strand at 112.688 kb on + strand at 112.825 kb on + strand, within fdnI at 112.831 kb on - strand, within fdnI at 112.839 kb on - strand, within fdnI at 112.990 kb on - strand, within fdnI at 112.990 kb on - strand, within fdnI at 113.024 kb on - strand, within fdnI at 113.060 kb on - strand, within fdnI at 113.078 kb on + strand, within fdnI at 113.086 kb on - strand, within fdnI at 113.086 kb on - strand at 113.165 kb on + strand, within fdnI at 113.165 kb on + strand, within fdnI at 113.171 kb on - strand, within fdnI at 113.200 kb on + strand, within fdnI at 113.299 kb on + strand at 113.332 kb on + strand at 113.340 kb on - strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.349 kb on + strand at 113.357 kb on - strand at 113.357 kb on - strand at 113.357 kb on - strand at 113.364 kb on + strand, within fdhE at 113.428 kb on - strand, within fdhE at 113.428 kb on - strand, within fdhE at 113.472 kb on + strand, within fdhE at 113.583 kb on - strand, within fdhE at 113.608 kb on + strand, within fdhE
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 110,757 + fdnG SO0101 0.68 -1.1 110,764 - fdnG SO0101 0.68 +1.2 110,764 - fdnG SO0101 0.68 +1.3 110,764 - fdnG SO0101 0.68 -1.4 110,764 - fdnG SO0101 0.68 +0.9 110,790 - fdnG SO0101 0.69 -1.5 110,849 + fdnG SO0101 0.71 -0.2 110,872 - fdnG SO0101 0.72 -0.7 110,937 + fdnG SO0101 0.74 -1.8 110,945 - fdnG SO0101 0.74 -0.9 110,982 - fdnG SO0101 0.76 +0.4 110,994 + fdnG SO0101 0.76 +0.2 111,000 - fdnG SO0101 0.76 -0.4 111,002 - fdnG SO0101 0.76 +0.6 111,027 + fdnG SO0101 0.77 +0.4 111,032 + fdnG SO0101 0.77 +0.4 111,050 - fdnG SO0101 0.78 -0.1 111,080 + fdnG SO0101 0.79 -0.3 111,088 - fdnG SO0101 0.79 -0.2 111,088 - fdnG SO0101 0.79 -0.2 111,088 - fdnG SO0101 0.79 -0.2 111,088 - fdnG SO0101 0.79 -0.5 111,145 - fdnG SO0101 0.81 +0.3 111,191 - fdnG SO0101 0.83 +0.1 111,191 - fdnG SO0101 0.83 +0.3 111,262 + fdnG SO0101 0.85 -0.6 111,264 + fdnG SO0101 0.85 +0.6 111,264 + fdnG SO0101 0.85 +0.1 111,264 + fdnG SO0101 0.85 +0.7 111,264 + fdnG SO0101 0.85 -0.7 111,269 + fdnG SO0101 0.85 -0.7 111,269 + fdnG SO0101 0.85 +1.2 111,269 + fdnG SO0101 0.85 +0.6 111,269 + fdnG SO0101 0.85 -0.3 111,272 - fdnG SO0101 0.85 +0.2 111,272 - fdnG SO0101 0.85 +0.0 111,272 - fdnG SO0101 0.85 -0.7 111,272 - fdnG SO0101 0.85 +0.7 111,277 - fdnG SO0101 0.85 +0.7 111,277 - fdnG SO0101 0.85 +0.2 111,277 - fdnG SO0101 0.85 +0.1 111,277 - fdnG SO0101 0.85 +0.1 111,277 - fdnG SO0101 0.85 -0.1 111,277 - fdnG SO0101 0.85 +0.8 111,277 - fdnG SO0101 0.85 +0.2 111,277 - fdnG SO0101 0.85 -0.5 111,277 - fdnG SO0101 0.85 +0.1 111,328 + fdnG SO0101 0.87 -0.7 111,330 - fdnG SO0101 0.87 +0.7 111,336 - fdnG SO0101 0.87 -2.2 111,409 + fdnG SO0101 0.90 -0.4 111,409 + fdnG SO0101 0.90 -0.0 111,522 + +0.4 111,536 + +0.1 111,675 + -0.4 111,675 - +0.1 111,711 + -0.2 111,756 + -1.1 111,764 - +0.4 111,787 - +0.0 111,823 + fdnH SO0102 0.11 -0.4 111,841 + fdnH SO0102 0.13 -0.1 111,871 + fdnH SO0102 0.17 -0.3 111,871 + fdnH SO0102 0.17 -1.1 111,871 + fdnH SO0102 0.17 -0.5 111,941 + fdnH SO0102 0.24 -0.2 112,014 + fdnH SO0102 0.32 +1.0 112,022 - fdnH SO0102 0.33 +0.1 112,022 - fdnH SO0102 0.33 -0.2 112,163 + fdnH SO0102 0.48 -0.9 112,185 + fdnH SO0102 0.51 -0.6 112,212 - fdnH SO0102 0.54 -0.9 112,247 + fdnH SO0102 0.58 -0.4 112,247 + fdnH SO0102 0.58 +1.0 112,250 - fdnH SO0102 0.58 -0.3 112,293 + fdnH SO0102 0.63 -0.8 112,355 + fdnH SO0102 0.69 -2.5 112,355 + fdnH SO0102 0.69 -0.6 112,368 + fdnH SO0102 0.71 +0.2 112,368 + fdnH SO0102 0.71 +0.1 112,375 - fdnH SO0102 0.72 -0.3 112,423 - fdnH SO0102 0.77 -2.0 112,423 - fdnH SO0102 0.77 -0.5 112,467 - fdnH SO0102 0.82 -0.4 112,521 + fdnH SO0102 0.88 -0.4 112,597 + -0.9 112,598 + +0.1 112,665 + -1.0 112,665 + -0.2 112,688 + -0.4 112,825 + fdnI SO0103 0.30 -1.1 112,831 - fdnI SO0103 0.31 +0.5 112,839 - fdnI SO0103 0.32 +0.0 112,990 - fdnI SO0103 0.56 +0.6 112,990 - fdnI SO0103 0.56 -0.3 113,024 - fdnI SO0103 0.61 +0.3 113,060 - fdnI SO0103 0.67 +0.8 113,078 + fdnI SO0103 0.70 -0.7 113,086 - fdnI SO0103 0.71 -0.4 113,086 - +0.7 113,165 + fdnI SO0103 0.83 -0.1 113,165 + fdnI SO0103 0.83 -0.0 113,171 - fdnI SO0103 0.84 +0.2 113,200 + fdnI SO0103 0.89 +1.3 113,299 + -0.7 113,332 + -1.3 113,340 - +1.1 113,349 + -0.5 113,349 + -2.3 113,349 + -0.5 113,349 + -0.6 113,349 + -1.7 113,357 - -0.8 113,357 - -0.8 113,357 - -0.3 113,364 + fdhE SO0104 0.10 +0.4 113,428 - fdhE SO0104 0.17 -4.2 113,428 - fdhE SO0104 0.17 -0.6 113,472 + fdhE SO0104 0.22 +0.6 113,583 - fdhE SO0104 0.34 -3.9 113,608 + fdhE SO0104 0.37 +0.2
Or see this region's nucleotide sequence