Experiment: m.b. Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05500 and PGA1_c05510 are separated by 105 nucleotides PGA1_c05510 and PGA1_c05520 are separated by 48 nucleotides PGA1_c05520 and PGA1_c05530 are separated by 111 nucleotides
PGA1_c05500: PGA1_c05500 - putative purine permease, at 561,691 to 563,118
_c05500
PGA1_c05510: PGA1_c05510 - glyceraldehyde-3-phosphate dehydrogenase 1, at 563,224 to 564,285
_c05510
PGA1_c05520: PGA1_c05520 - hypothetical protein, at 564,334 to 564,834
_c05520
PGA1_c05530: PGA1_c05530 - putative allantoin catabolism protein, at 564,946 to 565,938
_c05530
Position (kb)
563
564
565 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 562.256 kb on + strand, within PGA1_c05500 at 562.286 kb on + strand, within PGA1_c05500 at 562.286 kb on + strand, within PGA1_c05500 at 562.291 kb on + strand, within PGA1_c05500 at 562.383 kb on + strand, within PGA1_c05500 at 562.383 kb on + strand, within PGA1_c05500 at 562.391 kb on - strand, within PGA1_c05500 at 562.420 kb on + strand, within PGA1_c05500 at 562.427 kb on + strand, within PGA1_c05500 at 562.435 kb on - strand, within PGA1_c05500 at 562.481 kb on + strand, within PGA1_c05500 at 562.509 kb on - strand, within PGA1_c05500 at 562.641 kb on - strand, within PGA1_c05500 at 562.647 kb on + strand, within PGA1_c05500 at 562.647 kb on + strand, within PGA1_c05500 at 562.664 kb on + strand, within PGA1_c05500 at 562.683 kb on - strand, within PGA1_c05500 at 562.739 kb on - strand, within PGA1_c05500 at 562.755 kb on + strand, within PGA1_c05500 at 562.918 kb on - strand, within PGA1_c05500 at 562.945 kb on + strand, within PGA1_c05500 at 562.953 kb on - strand, within PGA1_c05500 at 562.956 kb on + strand, within PGA1_c05500 at 562.982 kb on + strand at 562.982 kb on + strand at 562.982 kb on + strand at 562.990 kb on - strand at 562.990 kb on - strand at 562.992 kb on + strand at 563.012 kb on + strand at 563.017 kb on + strand at 563.017 kb on + strand at 563.025 kb on - strand at 563.086 kb on + strand at 563.092 kb on + strand at 563.099 kb on - strand at 563.123 kb on + strand at 563.123 kb on + strand at 563.128 kb on + strand at 563.133 kb on + strand at 563.135 kb on - strand at 563.136 kb on - strand at 563.376 kb on + strand, within PGA1_c05510 at 563.376 kb on + strand, within PGA1_c05510 at 563.381 kb on + strand, within PGA1_c05510 at 563.384 kb on - strand, within PGA1_c05510 at 563.384 kb on - strand, within PGA1_c05510 at 563.410 kb on - strand, within PGA1_c05510 at 563.410 kb on - strand, within PGA1_c05510 at 563.488 kb on - strand, within PGA1_c05510 at 563.649 kb on + strand, within PGA1_c05510 at 563.734 kb on - strand, within PGA1_c05510 at 563.734 kb on - strand, within PGA1_c05510 at 563.735 kb on + strand, within PGA1_c05510 at 563.782 kb on - strand, within PGA1_c05510 at 563.831 kb on - strand, within PGA1_c05510 at 563.860 kb on + strand, within PGA1_c05510 at 563.904 kb on + strand, within PGA1_c05510 at 563.904 kb on + strand, within PGA1_c05510 at 563.958 kb on + strand, within PGA1_c05510 at 563.981 kb on - strand, within PGA1_c05510 at 563.981 kb on - strand, within PGA1_c05510 at 564.026 kb on - strand, within PGA1_c05510 at 564.026 kb on - strand, within PGA1_c05510 at 564.054 kb on + strand, within PGA1_c05510 at 564.062 kb on + strand, within PGA1_c05510 at 564.062 kb on - strand, within PGA1_c05510 at 564.102 kb on - strand, within PGA1_c05510 at 564.163 kb on + strand, within PGA1_c05510 at 564.163 kb on + strand, within PGA1_c05510 at 564.215 kb on - strand at 564.227 kb on - strand at 564.227 kb on - strand at 564.227 kb on - strand at 564.227 kb on - strand at 564.227 kb on - strand at 564.328 kb on - strand at 564.338 kb on + strand at 564.343 kb on + strand at 564.522 kb on - strand, within PGA1_c05520 at 564.600 kb on - strand, within PGA1_c05520 at 564.678 kb on - strand, within PGA1_c05520 at 564.693 kb on + strand, within PGA1_c05520 at 564.712 kb on + strand, within PGA1_c05520 at 564.743 kb on + strand, within PGA1_c05520 at 564.762 kb on + strand, within PGA1_c05520 at 564.807 kb on - strand at 564.850 kb on - strand at 564.850 kb on - strand at 564.850 kb on - strand at 564.850 kb on - strand at 564.850 kb on - strand at 564.865 kb on - strand at 564.893 kb on + strand at 564.900 kb on + strand at 564.901 kb on - strand at 564.939 kb on + strand at 564.947 kb on - strand at 564.947 kb on - strand at 564.947 kb on - strand at 564.947 kb on - strand at 564.947 kb on - strand at 564.961 kb on + strand at 564.974 kb on - strand at 565.007 kb on + strand at 565.153 kb on - strand, within PGA1_c05530 at 565.180 kb on - strand, within PGA1_c05530 at 565.186 kb on + strand, within PGA1_c05530
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.008 mg/ml remove 562,256 + PGA1_c05500 0.40 -0.1 562,286 + PGA1_c05500 0.42 +1.8 562,286 + PGA1_c05500 0.42 -1.0 562,291 + PGA1_c05500 0.42 +0.4 562,383 + PGA1_c05500 0.48 -0.0 562,383 + PGA1_c05500 0.48 -0.0 562,391 - PGA1_c05500 0.49 -0.3 562,420 + PGA1_c05500 0.51 +1.2 562,427 + PGA1_c05500 0.52 +1.0 562,435 - PGA1_c05500 0.52 -0.2 562,481 + PGA1_c05500 0.55 -0.8 562,509 - PGA1_c05500 0.57 -0.7 562,641 - PGA1_c05500 0.67 +0.2 562,647 + PGA1_c05500 0.67 +0.1 562,647 + PGA1_c05500 0.67 -0.0 562,664 + PGA1_c05500 0.68 +2.3 562,683 - PGA1_c05500 0.69 +0.8 562,739 - PGA1_c05500 0.73 -0.4 562,755 + PGA1_c05500 0.75 +1.1 562,918 - PGA1_c05500 0.86 -0.7 562,945 + PGA1_c05500 0.88 -2.3 562,953 - PGA1_c05500 0.88 +0.1 562,956 + PGA1_c05500 0.89 -2.7 562,982 + +2.0 562,982 + -0.0 562,982 + -0.7 562,990 - +1.0 562,990 - -0.0 562,992 + +1.6 563,012 + +0.1 563,017 + -1.0 563,017 + +0.6 563,025 - -0.8 563,086 + +1.6 563,092 + -0.6 563,099 - +0.3 563,123 + -1.0 563,123 + -0.8 563,128 + -2.1 563,133 + +0.2 563,135 - -0.5 563,136 - -1.8 563,376 + PGA1_c05510 0.14 -0.4 563,376 + PGA1_c05510 0.14 +0.6 563,381 + PGA1_c05510 0.15 +1.0 563,384 - PGA1_c05510 0.15 +0.6 563,384 - PGA1_c05510 0.15 -1.0 563,410 - PGA1_c05510 0.18 +0.1 563,410 - PGA1_c05510 0.18 -0.0 563,488 - PGA1_c05510 0.25 -1.0 563,649 + PGA1_c05510 0.40 +1.0 563,734 - PGA1_c05510 0.48 -0.6 563,734 - PGA1_c05510 0.48 +0.6 563,735 + PGA1_c05510 0.48 -1.2 563,782 - PGA1_c05510 0.53 -0.5 563,831 - PGA1_c05510 0.57 +1.0 563,860 + PGA1_c05510 0.60 -0.0 563,904 + PGA1_c05510 0.64 -0.2 563,904 + PGA1_c05510 0.64 +1.0 563,958 + PGA1_c05510 0.69 +0.4 563,981 - PGA1_c05510 0.71 -0.0 563,981 - PGA1_c05510 0.71 +1.0 564,026 - PGA1_c05510 0.76 -0.3 564,026 - PGA1_c05510 0.76 -0.0 564,054 + PGA1_c05510 0.78 +1.4 564,062 + PGA1_c05510 0.79 +1.3 564,062 - PGA1_c05510 0.79 -1.1 564,102 - PGA1_c05510 0.83 +1.7 564,163 + PGA1_c05510 0.88 +1.2 564,163 + PGA1_c05510 0.88 +1.6 564,215 - -0.5 564,227 - -0.6 564,227 - -2.8 564,227 - -0.5 564,227 - +1.0 564,227 - +0.7 564,328 - +0.7 564,338 + +0.5 564,343 + +1.8 564,522 - PGA1_c05520 0.38 +1.0 564,600 - PGA1_c05520 0.53 -0.3 564,678 - PGA1_c05520 0.69 -0.4 564,693 + PGA1_c05520 0.72 +0.6 564,712 + PGA1_c05520 0.75 -0.4 564,743 + PGA1_c05520 0.82 +1.5 564,762 + PGA1_c05520 0.85 +1.0 564,807 - +0.2 564,850 - -0.0 564,850 - -0.3 564,850 - +1.0 564,850 - +1.0 564,850 - -0.2 564,865 - +1.1 564,893 + +0.7 564,900 + +2.3 564,901 - +0.4 564,939 + +1.0 564,947 - -1.3 564,947 - -0.6 564,947 - +0.2 564,947 - +2.0 564,947 - +0.2 564,961 + +0.6 564,974 - +0.7 565,007 + -1.0 565,153 - PGA1_c05530 0.21 +0.4 565,180 - PGA1_c05530 0.24 +1.8 565,186 + PGA1_c05530 0.24 +1.3
Or see this region's nucleotide sequence