Experiment: butyrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2318 and Psest_2319 are separated by 118 nucleotides Psest_2319 and Psest_2320 are separated by 74 nucleotides
Psest_2318: Psest_2318 - probable AcnD-accessory protein PrpF, at 2,470,047 to 2,471,234
_2318
Psest_2319: Psest_2319 - aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent, at 2,471,353 to 2,473,956
_2319
Psest_2320: Psest_2320 - 2-methylcitrate synthase/citrate synthase II, at 2,474,031 to 2,475,158
_2320
Position (kb)
2471
2472
2473
2474 Strain fitness (log2 ratio)
-1
0
1
2 at 2471.224 kb on - strand at 2471.315 kb on - strand at 2471.315 kb on - strand at 2471.315 kb on - strand at 2474.078 kb on - strand at 2474.078 kb on - strand at 2474.078 kb on - strand at 2474.078 kb on - strand at 2474.218 kb on + strand, within Psest_2320 at 2474.288 kb on - strand, within Psest_2320 at 2474.368 kb on + strand, within Psest_2320 at 2474.368 kb on + strand, within Psest_2320 at 2474.539 kb on + strand, within Psest_2320 at 2474.753 kb on - strand, within Psest_2320 at 2474.753 kb on - strand, within Psest_2320 at 2474.753 kb on - strand, within Psest_2320 at 2474.891 kb on + strand, within Psest_2320 at 2474.953 kb on + strand, within Psest_2320 at 2474.953 kb on + strand, within Psest_2320
Per-strain Table
Position Strand Gene LocusTag Fraction butyrate (C) remove 2,471,224 - +1.5 2,471,315 - -0.7 2,471,315 - -1.0 2,471,315 - +2.1 2,474,078 - +1.0 2,474,078 - +1.8 2,474,078 - -0.6 2,474,078 - -1.3 2,474,218 + Psest_2320 0.17 -0.6 2,474,288 - Psest_2320 0.23 -0.6 2,474,368 + Psest_2320 0.30 +0.5 2,474,368 + Psest_2320 0.30 +0.2 2,474,539 + Psest_2320 0.45 -0.5 2,474,753 - Psest_2320 0.64 -0.6 2,474,753 - Psest_2320 0.64 +0.7 2,474,753 - Psest_2320 0.64 -0.1 2,474,891 + Psest_2320 0.76 +0.1 2,474,953 + Psest_2320 0.82 -0.6 2,474,953 + Psest_2320 0.82 -0.5
Or see this region's nucleotide sequence