Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3267 and Dsui_3268 are separated by 397 nucleotides Dsui_3268 and Dsui_3269 are separated by 103 nucleotides Dsui_3269 and Dsui_3270 are separated by 71 nucleotides
Dsui_3267: Dsui_3267 - biopolymer transport protein, at 3,505,809 to 3,506,414
_3267
Dsui_3268: Dsui_3268 - exodeoxyribonuclease VII, large subunit, at 3,506,812 to 3,508,212
_3268
Dsui_3269: Dsui_3269 - superoxide dismutase, at 3,508,316 to 3,508,903
_3269
Dsui_3270: Dsui_3270 - 2'-5' RNA ligase, at 3,508,975 to 3,509,652
_3270
Position (kb)
3506
3507
3508
3509 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3506.576 kb on + strand at 3506.576 kb on + strand at 3506.661 kb on - strand at 3506.704 kb on - strand at 3506.769 kb on + strand at 3506.777 kb on - strand at 3506.777 kb on - strand at 3506.777 kb on - strand at 3506.778 kb on - strand at 3506.817 kb on + strand at 3506.817 kb on + strand at 3506.817 kb on + strand at 3506.817 kb on + strand at 3506.825 kb on - strand at 3506.957 kb on - strand at 3507.028 kb on + strand, within Dsui_3268 at 3507.068 kb on - strand at 3507.126 kb on - strand at 3507.237 kb on + strand, within Dsui_3268 at 3507.383 kb on + strand, within Dsui_3268 at 3507.387 kb on + strand, within Dsui_3268 at 3507.462 kb on - strand at 3507.521 kb on + strand at 3507.728 kb on - strand at 3507.826 kb on - strand, within Dsui_3268 at 3507.826 kb on - strand, within Dsui_3268 at 3507.854 kb on + strand, within Dsui_3268 at 3507.963 kb on + strand, within Dsui_3268 at 3508.263 kb on + strand at 3508.271 kb on - strand at 3508.271 kb on - strand at 3508.271 kb on - strand at 3508.271 kb on - strand at 3508.275 kb on + strand at 3508.994 kb on + strand at 3508.999 kb on + strand at 3509.001 kb on - strand at 3509.002 kb on - strand at 3509.106 kb on - strand at 3509.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 3,506,576 + +0.1 3,506,576 + +1.4 3,506,661 - +3.4 3,506,704 - +1.8 3,506,769 + +2.4 3,506,777 - +3.4 3,506,777 - +2.4 3,506,777 - +0.8 3,506,778 - +1.4 3,506,817 + -0.6 3,506,817 + -0.0 3,506,817 + +0.1 3,506,817 + +0.5 3,506,825 - +1.1 3,506,957 - +2.4 3,507,028 + Dsui_3268 0.15 -0.6 3,507,068 - +1.4 3,507,126 - +2.4 3,507,237 + Dsui_3268 0.30 -0.2 3,507,383 + Dsui_3268 0.41 +0.5 3,507,387 + Dsui_3268 0.41 -0.1 3,507,462 - +0.4 3,507,521 + +1.4 3,507,728 - +0.4 3,507,826 - Dsui_3268 0.72 +0.8 3,507,826 - Dsui_3268 0.72 +2.4 3,507,854 + Dsui_3268 0.74 -1.5 3,507,963 + Dsui_3268 0.82 +0.5 3,508,263 + -0.5 3,508,271 - -1.1 3,508,271 - +1.4 3,508,271 - +2.4 3,508,271 - +1.4 3,508,275 + -0.6 3,508,994 + -0.4 3,508,999 + +0.5 3,509,001 - -0.2 3,509,002 - +0.1 3,509,106 - -0.4 3,509,106 - +0.8
Or see this region's nucleotide sequence