Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2809 and Dsui_2810 overlap by 4 nucleotides Dsui_2810 and Dsui_2811 are separated by 19 nucleotides Dsui_2811 and Dsui_2812 overlap by 4 nucleotides
Dsui_2809: Dsui_2809 - ABC-type uncharacterized transport system, auxiliary component, at 2,983,271 to 2,983,858
_2809
Dsui_2810: Dsui_2810 - ABC-type transport system involved in resistance to organic solvents, periplasmic component, at 2,983,855 to 2,984,778
_2810
Dsui_2811: Dsui_2811 - ABC-type transport system involved in resistance to organic solvents, ATPase component, at 2,984,798 to 2,985,571
_2811
Dsui_2812: Dsui_2812 - conserved hypothetical integral membrane protein, at 2,985,568 to 2,986,683
_2812
Position (kb)
2984
2985
2986 Strain fitness (log2 ratio)
-1
0
1
2 at 2983.827 kb on - strand at 2983.861 kb on - strand at 2984.498 kb on - strand, within Dsui_2810 at 2984.511 kb on + strand at 2984.718 kb on - strand at 2984.734 kb on - strand at 2984.737 kb on + strand at 2984.803 kb on - strand at 2984.994 kb on - strand, within Dsui_2811 at 2985.106 kb on - strand at 2985.161 kb on - strand, within Dsui_2811 at 2985.161 kb on - strand, within Dsui_2811 at 2985.281 kb on - strand, within Dsui_2811 at 2985.293 kb on - strand, within Dsui_2811 at 2985.438 kb on + strand at 2985.609 kb on - strand at 2985.646 kb on - strand at 2985.674 kb on - strand at 2985.685 kb on + strand at 2985.712 kb on + strand at 2985.793 kb on - strand, within Dsui_2812 at 2985.812 kb on + strand at 2985.847 kb on - strand, within Dsui_2812 at 2985.902 kb on + strand at 2985.927 kb on + strand at 2986.149 kb on - strand, within Dsui_2812 at 2986.155 kb on - strand, within Dsui_2812 at 2986.160 kb on - strand, within Dsui_2812 at 2986.198 kb on - strand, within Dsui_2812 at 2986.325 kb on - strand, within Dsui_2812 at 2986.500 kb on + strand at 2986.508 kb on - strand, within Dsui_2812 at 2986.508 kb on - strand at 2986.518 kb on + strand, within Dsui_2812
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,983,827 - +0.3 2,983,861 - -0.2 2,984,498 - Dsui_2810 0.70 +0.5 2,984,511 + +2.5 2,984,718 - -0.7 2,984,734 - +0.3 2,984,737 + +2.5 2,984,803 - -1.5 2,984,994 - Dsui_2811 0.25 -0.5 2,985,106 - +1.2 2,985,161 - Dsui_2811 0.47 +0.2 2,985,161 - Dsui_2811 0.47 -0.8 2,985,281 - Dsui_2811 0.62 +0.3 2,985,293 - Dsui_2811 0.64 -1.0 2,985,438 + +2.5 2,985,609 - -0.3 2,985,646 - +0.8 2,985,674 - +0.3 2,985,685 + +2.5 2,985,712 + +2.5 2,985,793 - Dsui_2812 0.20 -0.6 2,985,812 + +1.5 2,985,847 - Dsui_2812 0.25 +1.2 2,985,902 + +0.2 2,985,927 + +0.5 2,986,149 - Dsui_2812 0.52 +0.8 2,986,155 - Dsui_2812 0.53 -0.3 2,986,160 - Dsui_2812 0.53 -1.2 2,986,198 - Dsui_2812 0.56 -0.0 2,986,325 - Dsui_2812 0.68 -0.4 2,986,500 + +2.1 2,986,508 - Dsui_2812 0.84 +0.8 2,986,508 - +0.9 2,986,518 + Dsui_2812 0.85 -1.3
Or see this region's nucleotide sequence