Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2538 and Dsui_2539 are separated by 153 nucleotides Dsui_2539 and Dsui_2540 are separated by 24 nucleotides Dsui_2540 and Dsui_2541 overlap by 4 nucleotides Dsui_2541 and Dsui_2542 overlap by 4 nucleotides
Dsui_2538: Dsui_2538 - acetoacetyl-CoA reductase, at 2,690,074 to 2,690,814
_2538
Dsui_2539: Dsui_2539 - polyhydroxyalkanoate synthesis repressor PhaR, at 2,690,968 to 2,691,531
_2539
Dsui_2540: Dsui_2540 - ribosomal protein S12 methylthiotransferase RimO, at 2,691,556 to 2,692,899
_2540
Dsui_2541: Dsui_2541 - putative esterase of the alpha-beta hydrolase superfamily, at 2,692,896 to 2,693,765
_2541
Dsui_2542: Dsui_2542 - FAD/FMN-dependent dehydrogenase, at 2,693,762 to 2,695,168
_2542
Position (kb)
2691
2692
2693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2690.576 kb on - strand at 2690.640 kb on + strand, within Dsui_2538 at 2690.667 kb on + strand, within Dsui_2538 at 2690.675 kb on - strand, within Dsui_2538 at 2690.711 kb on + strand, within Dsui_2538 at 2690.737 kb on + strand, within Dsui_2538 at 2690.742 kb on - strand at 2690.747 kb on + strand at 2690.747 kb on + strand at 2690.811 kb on - strand at 2690.821 kb on + strand at 2690.964 kb on + strand at 2690.994 kb on + strand at 2691.046 kb on - strand, within Dsui_2539 at 2691.280 kb on + strand, within Dsui_2539 at 2691.344 kb on + strand, within Dsui_2539 at 2691.462 kb on - strand, within Dsui_2539 at 2691.550 kb on + strand at 2691.579 kb on + strand at 2692.216 kb on + strand, within Dsui_2540 at 2692.311 kb on + strand, within Dsui_2540 at 2692.863 kb on - strand at 2692.878 kb on + strand at 2692.909 kb on - strand at 2692.948 kb on - strand at 2693.131 kb on - strand, within Dsui_2541 at 2693.138 kb on + strand, within Dsui_2541 at 2693.227 kb on + strand, within Dsui_2541 at 2693.227 kb on + strand, within Dsui_2541 at 2693.242 kb on - strand, within Dsui_2541 at 2693.258 kb on + strand, within Dsui_2541 at 2693.363 kb on + strand, within Dsui_2541 at 2693.363 kb on + strand, within Dsui_2541 at 2693.368 kb on + strand, within Dsui_2541 at 2693.377 kb on + strand, within Dsui_2541 at 2693.387 kb on + strand, within Dsui_2541 at 2693.461 kb on + strand, within Dsui_2541 at 2693.622 kb on + strand, within Dsui_2541 at 2693.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,690,576 - +1.3 2,690,640 + Dsui_2538 0.76 +0.5 2,690,667 + Dsui_2538 0.80 +0.1 2,690,675 - Dsui_2538 0.81 -0.2 2,690,711 + Dsui_2538 0.86 +0.3 2,690,737 + Dsui_2538 0.89 +0.0 2,690,742 - +0.7 2,690,747 + -2.4 2,690,747 + -0.1 2,690,811 - +1.3 2,690,821 + +0.0 2,690,964 + -0.0 2,690,994 + +0.4 2,691,046 - Dsui_2539 0.14 +0.9 2,691,280 + Dsui_2539 0.55 +0.1 2,691,344 + Dsui_2539 0.67 -0.0 2,691,462 - Dsui_2539 0.88 +0.2 2,691,550 + +0.3 2,691,579 + -0.0 2,692,216 + Dsui_2540 0.49 -0.4 2,692,311 + Dsui_2540 0.56 -0.4 2,692,863 - +0.8 2,692,878 + +0.4 2,692,909 - +1.2 2,692,948 - -0.5 2,693,131 - Dsui_2541 0.27 -2.1 2,693,138 + Dsui_2541 0.28 +0.7 2,693,227 + Dsui_2541 0.38 +1.0 2,693,227 + Dsui_2541 0.38 +2.6 2,693,242 - Dsui_2541 0.40 +1.0 2,693,258 + Dsui_2541 0.42 +0.3 2,693,363 + Dsui_2541 0.54 -0.7 2,693,363 + Dsui_2541 0.54 +1.6 2,693,368 + Dsui_2541 0.54 +0.8 2,693,377 + Dsui_2541 0.55 -2.4 2,693,387 + Dsui_2541 0.56 +0.4 2,693,461 + Dsui_2541 0.65 -1.3 2,693,622 + Dsui_2541 0.83 -0.5 2,693,698 - -0.1
Or see this region's nucleotide sequence