Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2403 and Dsui_2404 are separated by 3 nucleotides Dsui_2404 and Dsui_2405 overlap by 4 nucleotides Dsui_2405 and Dsui_2406 are separated by 199 nucleotides Dsui_2406 and Dsui_2407 are separated by 12 nucleotides
Dsui_2403: Dsui_2403 - universal bacterial protein YeaZ, at 2,555,662 to 2,556,345
_2403
Dsui_2404: Dsui_2404 - ribosomal-protein-alanine acetyltransferase, at 2,556,349 to 2,556,840
_2404
Dsui_2405: Dsui_2405 - uracil-DNA glycosylase, family 4, at 2,556,837 to 2,557,694
_2405
Dsui_2406: Dsui_2406 - hypothetical protein, at 2,557,894 to 2,558,496
_2406
Dsui_2407: Dsui_2407 - beta-propeller domain-containing protein, methanol dehydrogenase, at 2,558,509 to 2,559,339
_2407
Position (kb)
2556
2557
2558 Strain fitness (log2 ratio)
-1
0
1
2 at 2556.354 kb on + strand at 2556.452 kb on + strand, within Dsui_2404 at 2556.517 kb on + strand, within Dsui_2404 at 2556.709 kb on + strand, within Dsui_2404 at 2556.715 kb on + strand, within Dsui_2404 at 2556.842 kb on + strand at 2557.699 kb on + strand at 2557.896 kb on - strand at 2557.896 kb on - strand at 2557.938 kb on + strand at 2557.941 kb on - strand at 2557.946 kb on - strand at 2557.976 kb on - strand at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.991 kb on - strand at 2557.991 kb on - strand at 2557.991 kb on - strand, within Dsui_2406 at 2558.064 kb on + strand, within Dsui_2406 at 2558.067 kb on - strand at 2558.072 kb on - strand at 2558.177 kb on + strand, within Dsui_2406 at 2558.195 kb on - strand at 2558.238 kb on + strand, within Dsui_2406 at 2558.243 kb on + strand, within Dsui_2406 at 2558.288 kb on + strand, within Dsui_2406 at 2558.420 kb on - strand, within Dsui_2406 at 2558.445 kb on + strand at 2558.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,556,354 + +0.8 2,556,452 + Dsui_2404 0.21 -0.1 2,556,517 + Dsui_2404 0.34 +0.4 2,556,709 + Dsui_2404 0.73 -0.6 2,556,715 + Dsui_2404 0.74 -0.5 2,556,842 + +0.6 2,557,699 + +0.7 2,557,896 - +2.5 2,557,896 - +1.5 2,557,938 + -0.3 2,557,941 - -0.6 2,557,946 - +2.5 2,557,976 - +1.5 2,557,983 + Dsui_2406 0.15 +0.8 2,557,983 + Dsui_2406 0.15 -0.4 2,557,983 + Dsui_2406 0.15 +0.0 2,557,983 + Dsui_2406 0.15 +0.5 2,557,991 - +0.9 2,557,991 - +1.5 2,557,991 - Dsui_2406 0.16 -0.3 2,558,064 + Dsui_2406 0.28 +0.1 2,558,067 - +0.9 2,558,072 - +1.5 2,558,177 + Dsui_2406 0.47 -0.1 2,558,195 - +0.2 2,558,238 + Dsui_2406 0.57 +0.3 2,558,243 + Dsui_2406 0.58 +0.2 2,558,288 + Dsui_2406 0.65 -1.0 2,558,420 - Dsui_2406 0.87 +1.8 2,558,445 + +0.4 2,558,448 + +0.9
Or see this region's nucleotide sequence