Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2391 and Dsui_2392 are separated by 2 nucleotides Dsui_2392 and Dsui_2393 overlap by 4 nucleotides Dsui_2393 and Dsui_2394 are separated by 3 nucleotides
Dsui_2391: Dsui_2391 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,543,525 to 2,544,013
_2391
Dsui_2392: Dsui_2392 - 2''-5'' RNA ligase, at 2,544,016 to 2,544,591
_2392
Dsui_2393: Dsui_2393 - uncharacterized domain HDIG-containing protein, at 2,544,588 to 2,545,811
_2393
Dsui_2394: Dsui_2394 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 2,545,815 to 2,548,775
_2394
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2544.017 kb on + strand at 2544.162 kb on - strand at 2544.192 kb on + strand, within Dsui_2392 at 2544.221 kb on - strand at 2544.255 kb on - strand at 2544.255 kb on - strand at 2544.315 kb on + strand, within Dsui_2392 at 2544.524 kb on + strand, within Dsui_2392 at 2544.574 kb on + strand at 2544.776 kb on - strand at 2544.896 kb on + strand, within Dsui_2393 at 2545.080 kb on + strand, within Dsui_2393 at 2545.309 kb on + strand, within Dsui_2393 at 2545.312 kb on + strand, within Dsui_2393 at 2545.444 kb on - strand at 2545.653 kb on + strand, within Dsui_2393 at 2545.871 kb on - strand at 2545.927 kb on + strand at 2545.970 kb on - strand at 2545.970 kb on - strand at 2545.974 kb on - strand at 2546.012 kb on + strand at 2546.098 kb on + strand at 2546.105 kb on + strand at 2546.438 kb on + strand, within Dsui_2394 at 2546.463 kb on + strand, within Dsui_2394 at 2546.533 kb on - strand at 2546.548 kb on + strand, within Dsui_2394
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,544,017 + +0.6 2,544,162 - +0.9 2,544,192 + Dsui_2392 0.31 +0.1 2,544,221 - +2.5 2,544,255 - +0.9 2,544,255 - +3.5 2,544,315 + Dsui_2392 0.52 -0.3 2,544,524 + Dsui_2392 0.88 -0.2 2,544,574 + -0.4 2,544,776 - +0.2 2,544,896 + Dsui_2393 0.25 +0.2 2,545,080 + Dsui_2393 0.40 -0.4 2,545,309 + Dsui_2393 0.59 +0.5 2,545,312 + Dsui_2393 0.59 -0.3 2,545,444 - +2.5 2,545,653 + Dsui_2393 0.87 -1.4 2,545,871 - +0.7 2,545,927 + +0.3 2,545,970 - -0.3 2,545,970 - +2.5 2,545,974 - +2.1 2,546,012 + +0.1 2,546,098 + -0.7 2,546,105 + -0.7 2,546,438 + Dsui_2394 0.21 +0.4 2,546,463 + Dsui_2394 0.22 +0.8 2,546,533 - +0.5 2,546,548 + Dsui_2394 0.25 +0.6
Or see this region's nucleotide sequence