Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2219 and Dsui_2220 are separated by 199 nucleotides Dsui_2220 and Dsui_2221 are separated by 271 nucleotides Dsui_2221 and Dsui_2222 are separated by 68 nucleotides
Dsui_2219: Dsui_2219 - dihydrolipoamide dehydrogenase, at 2,359,377 to 2,360,801
_2219
Dsui_2220: Dsui_2220 - hypothetical protein, at 2,361,001 to 2,361,321
_2220
Dsui_2221: Dsui_2221 - putative ATPase, at 2,361,593 to 2,362,708
_2221
Dsui_2222: Dsui_2222 - type II secretory pathway, component PulD, at 2,362,777 to 2,363,682
_2222
Position (kb)
2361
2362
2363 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2360.795 kb on + strand at 2360.795 kb on + strand at 2360.843 kb on + strand at 2360.946 kb on + strand at 2360.951 kb on + strand at 2360.959 kb on - strand at 2360.973 kb on + strand at 2361.024 kb on - strand at 2361.028 kb on - strand at 2361.092 kb on + strand, within Dsui_2220 at 2361.136 kb on + strand at 2361.141 kb on - strand, within Dsui_2220 at 2361.180 kb on + strand at 2361.180 kb on - strand, within Dsui_2220 at 2361.188 kb on - strand, within Dsui_2220 at 2361.279 kb on - strand, within Dsui_2220 at 2361.377 kb on + strand at 2361.476 kb on + strand at 2361.479 kb on - strand at 2361.484 kb on - strand at 2361.484 kb on - strand at 2361.491 kb on + strand at 2361.538 kb on - strand at 2361.565 kb on + strand at 2361.608 kb on - strand at 2361.680 kb on - strand at 2361.833 kb on - strand at 2361.917 kb on + strand, within Dsui_2221 at 2362.164 kb on + strand, within Dsui_2221 at 2362.233 kb on + strand, within Dsui_2221 at 2362.233 kb on + strand, within Dsui_2221 at 2362.302 kb on + strand, within Dsui_2221 at 2362.325 kb on - strand at 2362.475 kb on + strand, within Dsui_2221 at 2362.604 kb on + strand at 2362.606 kb on + strand at 2362.606 kb on + strand at 2362.614 kb on - strand at 2362.614 kb on - strand at 2362.774 kb on + strand at 2362.808 kb on + strand at 2362.818 kb on + strand at 2362.847 kb on - strand at 2362.851 kb on + strand at 2362.985 kb on + strand, within Dsui_2222 at 2363.048 kb on + strand, within Dsui_2222 at 2363.058 kb on + strand, within Dsui_2222 at 2363.073 kb on - strand, within Dsui_2222 at 2363.222 kb on + strand, within Dsui_2222 at 2363.222 kb on + strand, within Dsui_2222 at 2363.361 kb on + strand, within Dsui_2222 at 2363.420 kb on - strand, within Dsui_2222 at 2363.426 kb on + strand, within Dsui_2222 at 2363.473 kb on + strand, within Dsui_2222 at 2363.513 kb on + strand, within Dsui_2222
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,360,795 + +0.0 2,360,795 + -0.6 2,360,843 + -0.0 2,360,946 + +0.4 2,360,951 + +1.3 2,360,959 - -0.3 2,360,973 + +1.4 2,361,024 - +0.4 2,361,028 - +0.6 2,361,092 + Dsui_2220 0.28 +2.9 2,361,136 + +0.5 2,361,141 - Dsui_2220 0.44 +0.4 2,361,180 + +0.9 2,361,180 - Dsui_2220 0.56 +0.6 2,361,188 - Dsui_2220 0.58 +0.2 2,361,279 - Dsui_2220 0.87 +0.6 2,361,377 + +0.5 2,361,476 + +1.5 2,361,479 - +0.5 2,361,484 - +1.5 2,361,484 - -0.5 2,361,491 + -0.7 2,361,538 - +0.2 2,361,565 + +0.5 2,361,608 - +1.8 2,361,680 - +1.2 2,361,833 - -0.3 2,361,917 + Dsui_2221 0.29 -0.5 2,362,164 + Dsui_2221 0.51 +0.9 2,362,233 + Dsui_2221 0.57 -0.7 2,362,233 + Dsui_2221 0.57 -0.6 2,362,302 + Dsui_2221 0.64 -1.9 2,362,325 - +1.9 2,362,475 + Dsui_2221 0.79 -0.3 2,362,604 + -0.6 2,362,606 + +0.7 2,362,606 + -0.3 2,362,614 - +0.2 2,362,614 - +0.8 2,362,774 + +0.2 2,362,808 + -0.8 2,362,818 + -0.3 2,362,847 - -1.2 2,362,851 + +0.5 2,362,985 + Dsui_2222 0.23 -0.6 2,363,048 + Dsui_2222 0.30 -0.0 2,363,058 + Dsui_2222 0.31 -0.1 2,363,073 - Dsui_2222 0.33 -0.9 2,363,222 + Dsui_2222 0.49 -0.2 2,363,222 + Dsui_2222 0.49 -0.3 2,363,361 + Dsui_2222 0.64 +1.1 2,363,420 - Dsui_2222 0.71 -0.5 2,363,426 + Dsui_2222 0.72 +0.8 2,363,473 + Dsui_2222 0.77 +0.2 2,363,513 + Dsui_2222 0.81 -0.3
Or see this region's nucleotide sequence