Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2136 and Dsui_2137 are separated by 132 nucleotides Dsui_2137 and Dsui_2138 are separated by 35 nucleotides Dsui_2138 and Dsui_2139 are separated by 136 nucleotides Dsui_2139 and Dsui_2140 overlap by 4 nucleotides
Dsui_2136: Dsui_2136 - Fe-S oxidoreductase, at 2,265,531 to 2,266,382
_2136
Dsui_2137: Dsui_2137 - queuosine biosynthesis protein QueD, at 2,266,515 to 2,266,961
_2137
Dsui_2138: Dsui_2138 - putative esterase, at 2,266,997 to 2,267,665
_2138
Dsui_2139: Dsui_2139 - Protein of unknown function (DUF3348), at 2,267,802 to 2,268,530
_2139
Dsui_2140: Dsui_2140 - hypothetical protein, at 2,268,527 to 2,270,617
_2140
Position (kb)
2266
2267
2268 Strain fitness (log2 ratio)
-1
0
1
2 at 2266.422 kb on - strand at 2266.515 kb on + strand at 2266.518 kb on + strand at 2266.715 kb on + strand, within Dsui_2137 at 2266.790 kb on + strand, within Dsui_2137 at 2266.808 kb on + strand, within Dsui_2137 at 2266.960 kb on + strand at 2266.987 kb on + strand at 2267.040 kb on + strand at 2267.045 kb on + strand at 2267.073 kb on - strand at 2267.118 kb on + strand, within Dsui_2138 at 2267.232 kb on + strand, within Dsui_2138 at 2267.497 kb on + strand, within Dsui_2138 at 2267.610 kb on + strand at 2267.707 kb on - strand at 2267.744 kb on + strand at 2267.745 kb on + strand at 2267.799 kb on - strand at 2268.130 kb on - strand at 2268.176 kb on - strand at 2268.290 kb on + strand, within Dsui_2139 at 2268.303 kb on + strand, within Dsui_2139 at 2268.349 kb on + strand, within Dsui_2139 at 2268.354 kb on + strand, within Dsui_2139 at 2268.432 kb on + strand, within Dsui_2139 at 2268.435 kb on + strand, within Dsui_2139 at 2268.552 kb on + strand at 2268.582 kb on + strand at 2268.587 kb on + strand at 2268.592 kb on + strand at 2268.601 kb on - strand at 2268.644 kb on + strand at 2268.664 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 2,266,422 - -0.9 2,266,515 + +0.4 2,266,518 + +0.2 2,266,715 + Dsui_2137 0.45 -0.4 2,266,790 + Dsui_2137 0.62 -1.5 2,266,808 + Dsui_2137 0.66 +1.1 2,266,960 + +0.1 2,266,987 + -0.4 2,267,040 + -0.6 2,267,045 + -1.3 2,267,073 - +1.0 2,267,118 + Dsui_2138 0.18 -0.8 2,267,232 + Dsui_2138 0.35 -1.2 2,267,497 + Dsui_2138 0.75 -0.4 2,267,610 + -0.0 2,267,707 - +1.6 2,267,744 + -1.0 2,267,745 + -0.7 2,267,799 - +1.6 2,268,130 - +1.6 2,268,176 - +2.6 2,268,290 + Dsui_2139 0.67 -0.0 2,268,303 + Dsui_2139 0.69 -0.3 2,268,349 + Dsui_2139 0.75 +0.3 2,268,354 + Dsui_2139 0.76 -0.6 2,268,432 + Dsui_2139 0.86 +0.3 2,268,435 + Dsui_2139 0.87 +0.1 2,268,552 + -0.1 2,268,582 + +1.7 2,268,587 + +0.4 2,268,592 + -0.1 2,268,601 - -0.0 2,268,644 + +0.5 2,268,664 + -0.7
Or see this region's nucleotide sequence