Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1518 and Dsui_1519 are separated by 33 nucleotides Dsui_1519 and Dsui_1520 are separated by 86 nucleotides Dsui_1520 and Dsui_1521 are separated by 72 nucleotides Dsui_1521 and Dsui_1522 are separated by 14 nucleotides
Dsui_1518: Dsui_1518 - putative permease, at 1,580,859 to 1,581,980
_1518
Dsui_1519: Dsui_1519 - phosphoribosylaminoimidazole synthetase, at 1,582,014 to 1,583,063
_1519
Dsui_1520: Dsui_1520 - cytochrome c551/c552, at 1,583,150 to 1,583,467
_1520
Dsui_1521: Dsui_1521 - hypothetical protein, at 1,583,540 to 1,583,995
_1521
Dsui_1522: Dsui_1522 - serine/threonine protein kinase, at 1,584,010 to 1,585,344
_1522
Position (kb)
1582
1583
1584 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1581.051 kb on - strand, within Dsui_1518 at 1581.150 kb on + strand, within Dsui_1518 at 1581.202 kb on - strand, within Dsui_1518 at 1581.204 kb on - strand, within Dsui_1518 at 1581.245 kb on + strand, within Dsui_1518 at 1581.245 kb on + strand, within Dsui_1518 at 1581.253 kb on - strand, within Dsui_1518 at 1581.259 kb on - strand, within Dsui_1518 at 1581.341 kb on + strand, within Dsui_1518 at 1581.394 kb on - strand, within Dsui_1518 at 1581.464 kb on + strand at 1581.632 kb on - strand, within Dsui_1518 at 1581.910 kb on - strand at 1583.062 kb on - strand at 1583.084 kb on + strand at 1583.087 kb on - strand at 1583.150 kb on - strand at 1583.150 kb on - strand at 1583.182 kb on + strand, within Dsui_1520 at 1583.241 kb on - strand, within Dsui_1520 at 1583.241 kb on - strand, within Dsui_1520 at 1583.261 kb on + strand, within Dsui_1520 at 1583.271 kb on + strand, within Dsui_1520 at 1583.274 kb on - strand, within Dsui_1520 at 1583.291 kb on + strand, within Dsui_1520 at 1583.314 kb on - strand, within Dsui_1520 at 1583.426 kb on + strand, within Dsui_1520 at 1583.438 kb on - strand at 1583.469 kb on - strand at 1583.485 kb on - strand at 1583.485 kb on - strand at 1583.645 kb on + strand, within Dsui_1521 at 1583.675 kb on + strand at 1583.708 kb on + strand, within Dsui_1521 at 1583.732 kb on - strand, within Dsui_1521 at 1583.785 kb on - strand, within Dsui_1521 at 1583.974 kb on + strand at 1584.001 kb on - strand at 1584.030 kb on + strand at 1584.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 1,581,051 - Dsui_1518 0.17 -0.1 1,581,150 + Dsui_1518 0.26 -0.2 1,581,202 - Dsui_1518 0.31 -1.2 1,581,204 - Dsui_1518 0.31 -0.9 1,581,245 + Dsui_1518 0.34 -0.9 1,581,245 + Dsui_1518 0.34 -1.2 1,581,253 - Dsui_1518 0.35 -0.1 1,581,259 - Dsui_1518 0.36 -1.6 1,581,341 + Dsui_1518 0.43 -0.9 1,581,394 - Dsui_1518 0.48 +0.6 1,581,464 + +0.6 1,581,632 - Dsui_1518 0.69 -0.8 1,581,910 - -0.7 1,583,062 - -0.8 1,583,084 + +0.0 1,583,087 - -0.2 1,583,150 - -2.1 1,583,150 - +1.8 1,583,182 + Dsui_1520 0.10 +1.0 1,583,241 - Dsui_1520 0.29 -0.7 1,583,241 - Dsui_1520 0.29 -0.1 1,583,261 + Dsui_1520 0.35 +0.6 1,583,271 + Dsui_1520 0.38 -0.7 1,583,274 - Dsui_1520 0.39 -0.6 1,583,291 + Dsui_1520 0.44 -0.6 1,583,314 - Dsui_1520 0.52 +0.8 1,583,426 + Dsui_1520 0.87 +0.5 1,583,438 - +0.3 1,583,469 - +0.4 1,583,485 - +0.7 1,583,485 - -0.4 1,583,645 + Dsui_1521 0.23 -0.3 1,583,675 + +0.6 1,583,708 + Dsui_1521 0.37 -0.1 1,583,732 - Dsui_1521 0.42 -2.2 1,583,785 - Dsui_1521 0.54 +0.5 1,583,974 + +1.0 1,584,001 - -0.4 1,584,030 + +0.5 1,584,062 - +0.2
Or see this region's nucleotide sequence