Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0741 and Dsui_0742 are separated by 189 nucleotides Dsui_0742 and Dsui_0743 are separated by 88 nucleotides Dsui_0743 and Dsui_0744 are separated by 29 nucleotides Dsui_0744 and Dsui_0745 are separated by 199 nucleotides
Dsui_0741: Dsui_0741 - homoserine O-acetyltransferase, at 738,735 to 739,862
_0741
Dsui_0742: Dsui_0742 - phosphoenolpyruvate-protein phosphotransferase, at 740,052 to 741,791
_0742
Dsui_0743: Dsui_0743 - phosphotransferase system HPr (HPr) family protein, at 741,880 to 742,158
_0743
Dsui_0744: Dsui_0744 - phosphotransferase system, mannose/fructose-specific component IIA, at 742,188 to 742,580
_0744
Dsui_0745: Dsui_0745 - membrane-associated lipoprotein involved in thiamine biosynthesis, at 742,780 to 743,820
_0745
Position (kb) 740
741
742 Strain fitness (log2 ratio) -2
-1
0
1
2 at 739.938 kb on + strand at 739.940 kb on - strand at 739.967 kb on + strand at 739.998 kb on + strand at 740.031 kb on - strand at 740.098 kb on + strand at 740.101 kb on - strand at 740.112 kb on + strand at 740.128 kb on - strand at 740.266 kb on + strand, within Dsui_0742 at 740.274 kb on - strand, within Dsui_0742 at 740.302 kb on + strand, within Dsui_0742 at 740.381 kb on - strand, within Dsui_0742 at 740.532 kb on + strand, within Dsui_0742 at 740.549 kb on - strand, within Dsui_0742 at 740.559 kb on + strand, within Dsui_0742 at 740.601 kb on + strand, within Dsui_0742 at 740.646 kb on - strand, within Dsui_0742 at 740.646 kb on - strand, within Dsui_0742 at 740.702 kb on + strand, within Dsui_0742 at 740.721 kb on - strand, within Dsui_0742 at 740.731 kb on - strand, within Dsui_0742 at 740.756 kb on + strand, within Dsui_0742 at 740.764 kb on - strand, within Dsui_0742 at 740.949 kb on + strand, within Dsui_0742 at 740.957 kb on + strand, within Dsui_0742 at 740.962 kb on + strand, within Dsui_0742 at 740.989 kb on + strand, within Dsui_0742 at 740.989 kb on + strand, within Dsui_0742 at 741.139 kb on + strand, within Dsui_0742 at 741.167 kb on + strand at 741.170 kb on + strand, within Dsui_0742 at 741.188 kb on - strand, within Dsui_0742 at 741.193 kb on - strand, within Dsui_0742 at 741.277 kb on - strand, within Dsui_0742 at 741.286 kb on + strand, within Dsui_0742 at 741.407 kb on + strand, within Dsui_0742 at 741.421 kb on - strand, within Dsui_0742 at 741.438 kb on - strand, within Dsui_0742 at 741.542 kb on + strand, within Dsui_0742 at 741.586 kb on + strand, within Dsui_0742 at 741.615 kb on - strand, within Dsui_0742 at 741.617 kb on + strand, within Dsui_0742 at 741.693 kb on + strand at 741.707 kb on + strand at 741.715 kb on - strand at 741.738 kb on - strand at 741.798 kb on + strand at 741.806 kb on + strand at 741.921 kb on - strand, within Dsui_0743 at 741.921 kb on - strand, within Dsui_0743 at 741.957 kb on + strand, within Dsui_0743 at 741.965 kb on - strand, within Dsui_0743 at 742.049 kb on - strand, within Dsui_0743 at 742.147 kb on + strand at 742.152 kb on + strand at 742.193 kb on - strand at 742.194 kb on - strand at 742.199 kb on - strand at 742.236 kb on - strand, within Dsui_0744 at 742.295 kb on - strand, within Dsui_0744 at 742.324 kb on + strand, within Dsui_0744 at 742.511 kb on - strand, within Dsui_0744 at 742.530 kb on - strand, within Dsui_0744 at 742.532 kb on + strand, within Dsui_0744 at 742.584 kb on + strand at 742.644 kb on + strand at 742.668 kb on + strand at 742.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 739,938 + -0.2 739,940 - -2.1 739,967 + +1.7 739,998 + +0.4 740,031 - +0.5 740,098 + -0.9 740,101 - -0.4 740,112 + -0.5 740,128 - -0.5 740,266 + Dsui_0742 0.12 -2.2 740,274 - Dsui_0742 0.13 -0.4 740,302 + Dsui_0742 0.14 +0.1 740,381 - Dsui_0742 0.19 -1.0 740,532 + Dsui_0742 0.28 +0.8 740,549 - Dsui_0742 0.29 -0.3 740,559 + Dsui_0742 0.29 -0.3 740,601 + Dsui_0742 0.32 -0.0 740,646 - Dsui_0742 0.34 -1.1 740,646 - Dsui_0742 0.34 -0.2 740,702 + Dsui_0742 0.37 +0.2 740,721 - Dsui_0742 0.38 -1.1 740,731 - Dsui_0742 0.39 -0.5 740,756 + Dsui_0742 0.40 -0.8 740,764 - Dsui_0742 0.41 -0.8 740,949 + Dsui_0742 0.52 +0.3 740,957 + Dsui_0742 0.52 -1.0 740,962 + Dsui_0742 0.52 -0.1 740,989 + Dsui_0742 0.54 -0.4 740,989 + Dsui_0742 0.54 -1.0 741,139 + Dsui_0742 0.62 +0.1 741,167 + +0.7 741,170 + Dsui_0742 0.64 -0.1 741,188 - Dsui_0742 0.65 -0.8 741,193 - Dsui_0742 0.66 -0.8 741,277 - Dsui_0742 0.70 +0.1 741,286 + Dsui_0742 0.71 +0.7 741,407 + Dsui_0742 0.78 -1.6 741,421 - Dsui_0742 0.79 -0.7 741,438 - Dsui_0742 0.80 -0.8 741,542 + Dsui_0742 0.86 -0.3 741,586 + Dsui_0742 0.88 -0.8 741,615 - Dsui_0742 0.90 -1.3 741,617 + Dsui_0742 0.90 +0.1 741,693 + -1.2 741,707 + +1.8 741,715 - -0.4 741,738 - -0.9 741,798 + +0.9 741,806 + +0.1 741,921 - Dsui_0743 0.15 -1.0 741,921 - Dsui_0743 0.15 -1.0 741,957 + Dsui_0743 0.28 +0.7 741,965 - Dsui_0743 0.30 -0.2 742,049 - Dsui_0743 0.61 -0.4 742,147 + -1.4 742,152 + +1.4 742,193 - +0.5 742,194 - -0.2 742,199 - +0.4 742,236 - Dsui_0744 0.12 -0.5 742,295 - Dsui_0744 0.27 -0.2 742,324 + Dsui_0744 0.35 +0.3 742,511 - Dsui_0744 0.82 +0.6 742,530 - Dsui_0744 0.87 +1.7 742,532 + Dsui_0744 0.88 -0.5 742,584 + -0.1 742,644 + +0.4 742,668 + -0.9 742,771 - -0.5
Or see this region's nucleotide sequence