Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0424 and Dsui_0425 overlap by 4 nucleotides Dsui_0425 and Dsui_0426 are separated by 6 nucleotides
Dsui_0424: Dsui_0424 - phosphoketolase, at 427,689 to 430,109
_0424
Dsui_0425: Dsui_0425 - putative Fe-S oxidoreductase, at 430,106 to 430,381
_0425
Dsui_0426: Dsui_0426 - transcriptional regulator, at 430,388 to 431,302
_0426
Position (kb)
430
431 Strain fitness (log2 ratio)
-1
0
1 at 429.124 kb on + strand, within Dsui_0424 at 429.288 kb on - strand at 429.306 kb on - strand, within Dsui_0424 at 429.352 kb on + strand, within Dsui_0424 at 429.381 kb on + strand, within Dsui_0424 at 429.384 kb on - strand, within Dsui_0424 at 429.442 kb on + strand, within Dsui_0424 at 429.522 kb on + strand, within Dsui_0424 at 429.676 kb on + strand, within Dsui_0424 at 429.715 kb on + strand, within Dsui_0424 at 429.758 kb on + strand, within Dsui_0424 at 429.758 kb on + strand, within Dsui_0424 at 429.776 kb on - strand, within Dsui_0424 at 429.877 kb on + strand at 429.885 kb on - strand at 429.915 kb on - strand at 429.915 kb on - strand at 429.917 kb on + strand at 429.943 kb on + strand at 430.020 kb on - strand at 430.020 kb on - strand at 430.060 kb on + strand at 430.096 kb on - strand at 430.103 kb on + strand at 430.118 kb on + strand at 430.218 kb on + strand, within Dsui_0425 at 430.235 kb on + strand, within Dsui_0425 at 430.439 kb on + strand at 430.558 kb on + strand, within Dsui_0426 at 430.575 kb on + strand, within Dsui_0426 at 430.583 kb on - strand, within Dsui_0426 at 430.600 kb on - strand, within Dsui_0426 at 430.652 kb on + strand, within Dsui_0426 at 430.655 kb on - strand, within Dsui_0426 at 430.695 kb on - strand, within Dsui_0426 at 430.700 kb on - strand, within Dsui_0426 at 430.811 kb on - strand, within Dsui_0426 at 430.878 kb on - strand, within Dsui_0426 at 430.883 kb on - strand, within Dsui_0426 at 430.996 kb on - strand, within Dsui_0426 at 431.055 kb on + strand at 431.182 kb on + strand, within Dsui_0426 at 431.191 kb on - strand, within Dsui_0426 at 431.233 kb on - strand at 431.254 kb on - strand at 431.307 kb on - strand at 431.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 429,124 + Dsui_0424 0.59 -0.2 429,288 - +1.6 429,306 - Dsui_0424 0.67 +0.0 429,352 + Dsui_0424 0.69 -0.4 429,381 + Dsui_0424 0.70 -0.4 429,384 - Dsui_0424 0.70 -0.2 429,442 + Dsui_0424 0.72 -0.6 429,522 + Dsui_0424 0.76 -0.4 429,676 + Dsui_0424 0.82 -0.9 429,715 + Dsui_0424 0.84 +0.4 429,758 + Dsui_0424 0.85 +0.7 429,758 + Dsui_0424 0.85 -1.1 429,776 - Dsui_0424 0.86 -0.8 429,877 + -0.4 429,885 - +0.3 429,915 - +1.7 429,915 - +0.4 429,917 + -0.7 429,943 + +1.0 430,020 - +0.5 430,020 - +0.2 430,060 + -0.9 430,096 - +0.2 430,103 + +0.0 430,118 + -0.6 430,218 + Dsui_0425 0.41 -0.7 430,235 + Dsui_0425 0.47 +0.6 430,439 + -0.3 430,558 + Dsui_0426 0.19 -0.0 430,575 + Dsui_0426 0.20 +0.2 430,583 - Dsui_0426 0.21 -0.1 430,600 - Dsui_0426 0.23 -0.1 430,652 + Dsui_0426 0.29 -0.1 430,655 - Dsui_0426 0.29 -0.3 430,695 - Dsui_0426 0.34 +0.1 430,700 - Dsui_0426 0.34 -0.9 430,811 - Dsui_0426 0.46 +0.6 430,878 - Dsui_0426 0.54 -0.4 430,883 - Dsui_0426 0.54 +0.1 430,996 - Dsui_0426 0.66 -0.3 431,055 + +0.2 431,182 + Dsui_0426 0.87 +0.3 431,191 - Dsui_0426 0.88 -0.7 431,233 - +0.0 431,254 - +1.2 431,307 - +0.6 431,310 - -0.3
Or see this region's nucleotide sequence