Experiment: R2A with sodium pyruvate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0173 and Dsui_0174 overlap by 4 nucleotides Dsui_0174 and Dsui_0175 overlap by 1 nucleotides Dsui_0175 and Dsui_0176 are separated by 2 nucleotides Dsui_0176 and Dsui_0177 are separated by 171 nucleotides
Dsui_0173: Dsui_0173 - site-specific DNA methylase, at 175,738 to 177,546
_0173
Dsui_0174: Dsui_0174 - hypothetical protein, at 177,543 to 178,073
_0174
Dsui_0175: Dsui_0175 - hypothetical protein, at 178,073 to 179,881
_0175
Dsui_0176: Dsui_0176 - ribosome modulation factor, at 179,884 to 180,588
_0176
Dsui_0177: Dsui_0177 - hypothetical protein, at 180,760 to 181,641
_0177
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1
2 at 177.139 kb on + strand at 177.195 kb on + strand at 177.200 kb on + strand at 177.336 kb on + strand, within Dsui_0173 at 177.443 kb on + strand at 177.534 kb on - strand at 177.649 kb on - strand at 177.705 kb on + strand at 177.766 kb on + strand, within Dsui_0174 at 177.774 kb on - strand, within Dsui_0174 at 177.787 kb on + strand, within Dsui_0174 at 177.945 kb on + strand, within Dsui_0174 at 177.995 kb on - strand at 178.228 kb on + strand at 178.265 kb on + strand, within Dsui_0175 at 178.349 kb on + strand, within Dsui_0175 at 178.354 kb on + strand, within Dsui_0175 at 178.371 kb on - strand, within Dsui_0175 at 178.847 kb on - strand at 179.097 kb on + strand, within Dsui_0175 at 179.313 kb on + strand, within Dsui_0175 at 179.358 kb on - strand at 179.510 kb on + strand, within Dsui_0175 at 179.690 kb on + strand at 179.698 kb on - strand at 179.728 kb on - strand at 179.738 kb on + strand at 179.776 kb on + strand at 179.840 kb on + strand at 179.851 kb on - strand at 179.930 kb on + strand at 180.027 kb on - strand at 180.077 kb on + strand, within Dsui_0176 at 180.195 kb on + strand, within Dsui_0176 at 180.288 kb on - strand, within Dsui_0176 at 180.365 kb on - strand at 180.403 kb on - strand, within Dsui_0176 at 180.470 kb on + strand, within Dsui_0176 at 180.470 kb on + strand at 180.478 kb on - strand at 180.478 kb on - strand, within Dsui_0176 at 180.726 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium pyruvate remove 177,139 + +1.6 177,195 + +0.6 177,200 + +2.6 177,336 + Dsui_0173 0.88 +0.5 177,443 + +1.6 177,534 - +0.6 177,649 - +1.6 177,705 + +2.6 177,766 + Dsui_0174 0.42 -0.7 177,774 - Dsui_0174 0.44 +0.5 177,787 + Dsui_0174 0.46 +0.8 177,945 + Dsui_0174 0.76 -0.2 177,995 - +1.3 178,228 + +0.3 178,265 + Dsui_0175 0.11 -0.5 178,349 + Dsui_0175 0.15 +0.3 178,354 + Dsui_0175 0.16 +0.0 178,371 - Dsui_0175 0.16 -1.6 178,847 - +2.6 179,097 + Dsui_0175 0.57 +1.8 179,313 + Dsui_0175 0.69 +2.0 179,358 - +1.0 179,510 + Dsui_0175 0.79 +0.3 179,690 + +1.0 179,698 - +1.0 179,728 - +1.0 179,738 + -0.3 179,776 + +1.4 179,840 + -0.2 179,851 - +1.3 179,930 + -0.4 180,027 - +1.0 180,077 + Dsui_0176 0.27 -0.7 180,195 + Dsui_0176 0.44 -0.1 180,288 - Dsui_0176 0.57 -0.8 180,365 - +0.3 180,403 - Dsui_0176 0.74 +0.8 180,470 + Dsui_0176 0.83 -1.3 180,470 + +0.0 180,478 - +0.0 180,478 - Dsui_0176 0.84 +0.5 180,726 - +0.5
Or see this region's nucleotide sequence