Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valS and tRNA-Arg-8 are separated by 601 nucleotides tRNA-Arg-8 and tRNA-Ser-4 are separated by 95 nucleotides tRNA-Ser-4 and tRNA-Arg-7 are separated by 36 nucleotides tRNA-Arg-7 and tRNA-Arg-6 are separated by 43 nucleotides tRNA-Arg-6 and tRNA-Arg-5 are separated by 43 nucleotides tRNA-Arg-5 and tRNA-Arg-4 are separated by 83 nucleotides tRNA-Arg-4 and tRNA-Arg-3 are separated by 31 nucleotides tRNA-Arg-3 and tRNA-Ser-3 are separated by 20 nucleotides
SO3424: valS - valyl-tRNA synthetase (NCBI ptt file), at 3,563,491 to 3,566,367
valS
SO_t083: tRNA-Arg-8 - tRNA-Arg (RefSeq), at 3,566,969 to 3,567,045
tRNA-Arg-8
SO_t084: tRNA-Ser-4 - tRNA-Ser (RefSeq), at 3,567,141 to 3,567,232
tRNA-Ser-4
SO_t085: tRNA-Arg-7 - tRNA-Arg (RefSeq), at 3,567,269 to 3,567,345
tRNA-Arg-7
SO_t086: tRNA-Arg-6 - tRNA-Arg (RefSeq), at 3,567,389 to 3,567,465
tRNA-Arg-6
SO_t087: tRNA-Arg-5 - tRNA-Arg (RefSeq), at 3,567,509 to 3,567,585
tRNA-Arg-5
SO_t088: tRNA-Arg-4 - tRNA-Arg (RefSeq), at 3,567,669 to 3,567,745
tRNA-Arg-4
SO_t089: tRNA-Arg-3 - tRNA-Arg (RefSeq), at 3,567,777 to 3,567,853
tRNA-Arg-3
SO_t090: tRNA-Ser-3 - tRNA-Ser (RefSeq), at 3,567,874 to 3,567,965
tRNA-Ser-3
Position (kb) 3566
3567
3568 Strain fitness (log2 ratio) -1
0
1 at 3566.533 kb on + strand at 3566.541 kb on - strand at 3566.654 kb on + strand at 3566.662 kb on - strand at 3566.694 kb on - strand at 3566.694 kb on - strand at 3566.694 kb on - strand at 3566.701 kb on + strand at 3566.704 kb on - strand at 3566.709 kb on - strand at 3566.709 kb on - strand at 3566.716 kb on - strand at 3566.718 kb on - strand at 3566.830 kb on + strand at 3566.840 kb on - strand at 3566.840 kb on - strand at 3566.840 kb on - strand at 3566.840 kb on - strand at 3566.993 kb on - strand, within tRNA-Arg-8
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,566,533 + -0.1 3,566,541 - -0.3 3,566,654 + -0.9 3,566,662 - -0.1 3,566,694 - -0.0 3,566,694 - -1.4 3,566,694 - -1.0 3,566,701 + -1.6 3,566,704 - +0.4 3,566,709 - +0.1 3,566,709 - +0.5 3,566,716 - -0.0 3,566,718 - +0.1 3,566,830 + +0.0 3,566,840 - -0.9 3,566,840 - -0.1 3,566,840 - -0.1 3,566,840 - -0.9 3,566,993 - tRNA-Arg-8 SO_t083 0.31 -0.8
Or see this region's nucleotide sequence