Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argA and rpmG are separated by 304 nucleotides rpmG and rpmB are separated by 6 nucleotides rpmB and radC are separated by 121 nucleotides radC and dfp are separated by 122 nucleotides
SO4245: argA - amino-acid acetyltransferase (NCBI ptt file), at 4,423,096 to 4,424,433
argA
SO4246: rpmG - ribosomal protein L33 (NCBI ptt file), at 4,424,738 to 4,424,911
rpmG
SO4247: rpmB - ribosomal protein L28 (NCBI ptt file), at 4,424,918 to 4,425,154
rpmB
SO4248: radC - DNA repair protein RadC (NCBI ptt file), at 4,425,276 to 4,425,953
radC
SO4249: dfp - DNA/pantothenate metabolism flavoprotein (NCBI ptt file), at 4,426,076 to 4,427,302
dfp
Position (kb)
4425
4426 Strain fitness (log2 ratio)
-1
0
1 at 4424.329 kb on - strand at 4424.390 kb on + strand at 4424.395 kb on + strand at 4424.400 kb on + strand at 4424.408 kb on - strand at 4424.408 kb on - strand at 4424.421 kb on + strand at 4424.421 kb on + strand at 4424.421 kb on + strand at 4425.358 kb on - strand, within radC at 4425.443 kb on + strand, within radC at 4425.478 kb on - strand, within radC at 4425.494 kb on + strand, within radC at 4425.547 kb on + strand, within radC at 4425.584 kb on + strand, within radC at 4425.584 kb on + strand, within radC at 4425.584 kb on + strand, within radC at 4425.592 kb on - strand, within radC at 4425.592 kb on - strand, within radC at 4425.592 kb on - strand, within radC at 4425.658 kb on + strand, within radC at 4425.703 kb on + strand, within radC at 4425.711 kb on - strand, within radC at 4425.818 kb on + strand, within radC at 4425.818 kb on + strand, within radC at 4425.826 kb on - strand, within radC at 4425.826 kb on - strand, within radC at 4425.850 kb on - strand, within radC at 4425.907 kb on + strand at 4425.920 kb on + strand at 4425.928 kb on - strand at 4425.934 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,424,329 - -0.7 4,424,390 + -0.3 4,424,395 + -0.3 4,424,400 + -0.7 4,424,408 - +0.1 4,424,408 - -0.2 4,424,421 + +0.4 4,424,421 + +0.3 4,424,421 + -0.5 4,425,358 - radC SO4248 0.12 -0.6 4,425,443 + radC SO4248 0.25 +1.3 4,425,478 - radC SO4248 0.30 -0.1 4,425,494 + radC SO4248 0.32 +0.0 4,425,547 + radC SO4248 0.40 -0.3 4,425,584 + radC SO4248 0.45 -0.3 4,425,584 + radC SO4248 0.45 -1.0 4,425,584 + radC SO4248 0.45 -0.2 4,425,592 - radC SO4248 0.47 -0.4 4,425,592 - radC SO4248 0.47 -0.7 4,425,592 - radC SO4248 0.47 -0.5 4,425,658 + radC SO4248 0.56 -0.4 4,425,703 + radC SO4248 0.63 -0.0 4,425,711 - radC SO4248 0.64 +0.4 4,425,818 + radC SO4248 0.80 +0.4 4,425,818 + radC SO4248 0.80 +0.1 4,425,826 - radC SO4248 0.81 -0.2 4,425,826 - radC SO4248 0.81 -1.0 4,425,850 - radC SO4248 0.85 -1.0 4,425,907 + -0.6 4,425,920 + +0.2 4,425,928 - -0.1 4,425,934 + -0.1
Or see this region's nucleotide sequence