Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09160 and PGA1_c09170 overlap by 4 nucleotides PGA1_c09170 and PGA1_c09180 are separated by 105 nucleotides PGA1_c09180 and PGA1_c09200 are separated by 428 nucleotides
PGA1_c09160: PGA1_c09160 - Uncharacterized protein conserved in bacteria, at 941,798 to 942,418
_c09160
PGA1_c09170: PGA1_c09170 - putative biotin synthesis protein BioC, at 942,415 to 943,203
_c09170
PGA1_c09180: PGA1_c09180 - Protein of unknown function (DUF2478)., at 943,309 to 943,836
_c09180
PGA1_c09200: PGA1_c09200 - putative 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB, at 944,265 to 945,041
_c09200
Position (kb) 943
944 Strain fitness (log2 ratio) -2
-1
0
1
2 at 942.358 kb on + strand at 942.396 kb on + strand at 942.409 kb on + strand at 942.428 kb on - strand at 942.438 kb on - strand at 942.483 kb on - strand at 942.755 kb on - strand, within PGA1_c09170 at 942.756 kb on + strand, within PGA1_c09170 at 942.764 kb on + strand, within PGA1_c09170 at 942.846 kb on + strand, within PGA1_c09170 at 942.854 kb on - strand, within PGA1_c09170 at 942.860 kb on + strand, within PGA1_c09170 at 942.860 kb on + strand, within PGA1_c09170 at 942.868 kb on - strand, within PGA1_c09170 at 942.922 kb on - strand, within PGA1_c09170 at 942.943 kb on + strand, within PGA1_c09170 at 942.977 kb on + strand, within PGA1_c09170 at 943.009 kb on + strand, within PGA1_c09170 at 943.039 kb on + strand, within PGA1_c09170 at 943.047 kb on - strand, within PGA1_c09170 at 943.095 kb on - strand, within PGA1_c09170 at 943.166 kb on + strand at 943.167 kb on - strand at 943.174 kb on - strand at 943.202 kb on + strand at 943.214 kb on + strand at 943.262 kb on + strand at 943.317 kb on + strand at 943.325 kb on - strand at 943.325 kb on - strand at 943.388 kb on - strand, within PGA1_c09180 at 943.519 kb on - strand, within PGA1_c09180 at 943.559 kb on + strand, within PGA1_c09180 at 943.569 kb on + strand, within PGA1_c09180 at 943.593 kb on - strand, within PGA1_c09180 at 943.723 kb on + strand, within PGA1_c09180 at 943.788 kb on + strand at 943.794 kb on - strand at 944.043 kb on - strand at 944.216 kb on + strand at 944.235 kb on + strand at 944.235 kb on + strand at 944.329 kb on + strand at 944.337 kb on - strand at 944.337 kb on - strand at 944.377 kb on - strand, within PGA1_c09200 at 944.462 kb on - strand, within PGA1_c09200 at 944.537 kb on - strand, within PGA1_c09200 at 944.546 kb on + strand, within PGA1_c09200 at 944.554 kb on - strand, within PGA1_c09200 at 944.619 kb on - strand, within PGA1_c09200 at 944.703 kb on + strand, within PGA1_c09200 at 944.711 kb on - strand, within PGA1_c09200 at 944.711 kb on - strand, within PGA1_c09200 at 944.825 kb on + strand, within PGA1_c09200
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 942,358 + -0.2 942,396 + +1.0 942,409 + +0.0 942,428 - -0.4 942,438 - +0.0 942,483 - +2.2 942,755 - PGA1_c09170 0.43 -0.4 942,756 + PGA1_c09170 0.43 -1.2 942,764 + PGA1_c09170 0.44 -0.7 942,846 + PGA1_c09170 0.55 -0.1 942,854 - PGA1_c09170 0.56 +0.3 942,860 + PGA1_c09170 0.56 -0.6 942,860 + PGA1_c09170 0.56 +0.5 942,868 - PGA1_c09170 0.57 +1.9 942,922 - PGA1_c09170 0.64 -0.1 942,943 + PGA1_c09170 0.67 +2.2 942,977 + PGA1_c09170 0.71 +0.1 943,009 + PGA1_c09170 0.75 -0.4 943,039 + PGA1_c09170 0.79 -1.4 943,047 - PGA1_c09170 0.80 -0.1 943,095 - PGA1_c09170 0.86 +0.6 943,166 + +0.9 943,167 - -0.1 943,174 - -1.5 943,202 + +0.5 943,214 + -1.2 943,262 + +0.5 943,317 + +1.3 943,325 - +1.6 943,325 - +2.1 943,388 - PGA1_c09180 0.15 -1.3 943,519 - PGA1_c09180 0.40 -1.8 943,559 + PGA1_c09180 0.47 -0.4 943,569 + PGA1_c09180 0.49 +0.6 943,593 - PGA1_c09180 0.54 -0.1 943,723 + PGA1_c09180 0.78 -0.5 943,788 + -0.7 943,794 - +1.6 944,043 - +1.0 944,216 + +0.4 944,235 + -1.8 944,235 + +1.2 944,329 + -1.2 944,337 - +1.9 944,337 - -0.2 944,377 - PGA1_c09200 0.14 +1.3 944,462 - PGA1_c09200 0.25 -0.4 944,537 - PGA1_c09200 0.35 +1.2 944,546 + PGA1_c09200 0.36 -1.7 944,554 - PGA1_c09200 0.37 -0.4 944,619 - PGA1_c09200 0.46 +0.2 944,703 + PGA1_c09200 0.56 -1.4 944,711 - PGA1_c09200 0.57 -1.2 944,711 - PGA1_c09200 0.57 -0.7 944,825 + PGA1_c09200 0.72 +1.8
Or see this region's nucleotide sequence