Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00880 and PGA1_c00890 overlap by 4 nucleotides PGA1_c00890 and PGA1_c00900 are separated by 69 nucleotides PGA1_c00900 and PGA1_c00910 are separated by 12 nucleotides PGA1_c00910 and PGA1_c00920 are separated by 17 nucleotides PGA1_c00920 and PGA1_c00930 overlap by 4 nucleotides
PGA1_c00880: PGA1_c00880 - peptide deformylase Def, at 86,747 to 87,256
_c00880
PGA1_c00890: PGA1_c00890 - peptide deformylase Def, at 87,253 to 87,750
_c00890
PGA1_c00900: PGA1_c00900 - methionyl-tRNA formyltransferase Fmt, at 87,820 to 88,725
_c00900
PGA1_c00910: PGA1_c00910 - hypothetical protein, at 88,738 to 88,974
_c00910
PGA1_c00920: PGA1_c00920 - putative transporter, required for glycine utilization (from data) , at 88,992 to 89,615
_c00920
PGA1_c00930: PGA1_c00930 - ribonuclease H, at 89,612 to 90,079
_c00930
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 86.889 kb on + strand, within PGA1_c00880 at 86.934 kb on + strand, within PGA1_c00880 at 86.942 kb on - strand, within PGA1_c00880 at 86.957 kb on + strand, within PGA1_c00880 at 86.957 kb on + strand, within PGA1_c00880 at 86.962 kb on + strand, within PGA1_c00880 at 86.965 kb on - strand, within PGA1_c00880 at 86.982 kb on + strand, within PGA1_c00880 at 87.045 kb on + strand, within PGA1_c00880 at 87.045 kb on + strand, within PGA1_c00880 at 87.053 kb on - strand, within PGA1_c00880 at 87.053 kb on - strand, within PGA1_c00880 at 87.117 kb on + strand, within PGA1_c00880 at 87.117 kb on + strand, within PGA1_c00880 at 87.117 kb on + strand, within PGA1_c00880 at 87.117 kb on + strand, within PGA1_c00880 at 87.125 kb on - strand, within PGA1_c00880 at 87.145 kb on - strand, within PGA1_c00880 at 87.162 kb on + strand, within PGA1_c00880 at 87.162 kb on + strand, within PGA1_c00880 at 87.187 kb on - strand, within PGA1_c00880 at 87.189 kb on - strand, within PGA1_c00880 at 87.190 kb on + strand, within PGA1_c00880 at 87.195 kb on + strand, within PGA1_c00880 at 87.203 kb on - strand, within PGA1_c00880 at 87.249 kb on + strand at 87.257 kb on - strand at 87.257 kb on - strand at 87.268 kb on + strand at 87.273 kb on + strand at 87.281 kb on - strand at 87.310 kb on - strand, within PGA1_c00890 at 87.313 kb on - strand, within PGA1_c00890 at 87.341 kb on + strand, within PGA1_c00890 at 87.370 kb on - strand, within PGA1_c00890 at 87.383 kb on + strand, within PGA1_c00890 at 87.395 kb on + strand, within PGA1_c00890 at 87.430 kb on - strand, within PGA1_c00890 at 87.453 kb on + strand, within PGA1_c00890 at 87.463 kb on - strand, within PGA1_c00890 at 87.474 kb on - strand, within PGA1_c00890 at 87.526 kb on + strand, within PGA1_c00890 at 87.527 kb on + strand, within PGA1_c00890 at 87.535 kb on - strand, within PGA1_c00890 at 87.563 kb on - strand, within PGA1_c00890 at 87.592 kb on + strand, within PGA1_c00890 at 87.592 kb on + strand, within PGA1_c00890 at 87.592 kb on + strand, within PGA1_c00890 at 87.610 kb on - strand, within PGA1_c00890 at 87.696 kb on + strand, within PGA1_c00890 at 87.704 kb on - strand at 87.715 kb on + strand at 87.715 kb on + strand at 87.715 kb on + strand at 87.792 kb on + strand at 87.803 kb on + strand at 87.803 kb on + strand at 87.803 kb on + strand at 87.805 kb on + strand at 87.812 kb on + strand at 88.719 kb on + strand at 88.719 kb on - strand at 88.727 kb on + strand at 88.727 kb on - strand at 88.758 kb on - strand at 88.760 kb on + strand at 88.876 kb on + strand, within PGA1_c00910 at 88.884 kb on - strand, within PGA1_c00910 at 89.068 kb on - strand, within PGA1_c00920 at 89.072 kb on + strand, within PGA1_c00920 at 89.078 kb on - strand, within PGA1_c00920 at 89.080 kb on - strand, within PGA1_c00920 at 89.082 kb on + strand, within PGA1_c00920 at 89.082 kb on + strand, within PGA1_c00920 at 89.082 kb on + strand, within PGA1_c00920 at 89.088 kb on - strand, within PGA1_c00920 at 89.116 kb on - strand, within PGA1_c00920 at 89.116 kb on - strand, within PGA1_c00920 at 89.139 kb on - strand, within PGA1_c00920 at 89.186 kb on - strand, within PGA1_c00920 at 89.188 kb on + strand, within PGA1_c00920 at 89.271 kb on + strand, within PGA1_c00920 at 89.271 kb on + strand, within PGA1_c00920 at 89.271 kb on + strand, within PGA1_c00920 at 89.279 kb on - strand, within PGA1_c00920 at 89.302 kb on - strand, within PGA1_c00920 at 89.306 kb on - strand, within PGA1_c00920 at 89.369 kb on - strand, within PGA1_c00920 at 89.445 kb on - strand, within PGA1_c00920 at 89.558 kb on + strand at 89.558 kb on + strand at 89.576 kb on + strand at 89.579 kb on + strand at 89.600 kb on + strand at 89.609 kb on - strand at 89.639 kb on - strand at 89.640 kb on - strand at 89.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 86,889 + PGA1_c00880 0.28 -1.1 86,934 + PGA1_c00880 0.37 +0.1 86,942 - PGA1_c00880 0.38 -1.2 86,957 + PGA1_c00880 0.41 -0.9 86,957 + PGA1_c00880 0.41 -2.3 86,962 + PGA1_c00880 0.42 -0.8 86,965 - PGA1_c00880 0.43 -2.0 86,982 + PGA1_c00880 0.46 -2.6 87,045 + PGA1_c00880 0.58 -2.4 87,045 + PGA1_c00880 0.58 +0.3 87,053 - PGA1_c00880 0.60 -0.7 87,053 - PGA1_c00880 0.60 -1.1 87,117 + PGA1_c00880 0.73 -0.2 87,117 + PGA1_c00880 0.73 -1.9 87,117 + PGA1_c00880 0.73 -0.1 87,117 + PGA1_c00880 0.73 -0.5 87,125 - PGA1_c00880 0.74 -0.2 87,145 - PGA1_c00880 0.78 -1.4 87,162 + PGA1_c00880 0.81 +0.4 87,162 + PGA1_c00880 0.81 -2.9 87,187 - PGA1_c00880 0.86 -0.4 87,189 - PGA1_c00880 0.87 +0.4 87,190 + PGA1_c00880 0.87 -0.8 87,195 + PGA1_c00880 0.88 +0.8 87,203 - PGA1_c00880 0.89 -0.1 87,249 + -0.1 87,257 - +0.9 87,257 - -1.3 87,268 + -1.0 87,273 + +0.7 87,281 - -0.0 87,310 - PGA1_c00890 0.11 -0.5 87,313 - PGA1_c00890 0.12 -0.4 87,341 + PGA1_c00890 0.18 +0.8 87,370 - PGA1_c00890 0.23 +0.3 87,383 + PGA1_c00890 0.26 -0.4 87,395 + PGA1_c00890 0.29 -0.4 87,430 - PGA1_c00890 0.36 +0.6 87,453 + PGA1_c00890 0.40 +0.6 87,463 - PGA1_c00890 0.42 -1.1 87,474 - PGA1_c00890 0.44 -1.7 87,526 + PGA1_c00890 0.55 -1.5 87,527 + PGA1_c00890 0.55 +0.4 87,535 - PGA1_c00890 0.57 -1.4 87,563 - PGA1_c00890 0.62 +1.4 87,592 + PGA1_c00890 0.68 -0.8 87,592 + PGA1_c00890 0.68 -1.5 87,592 + PGA1_c00890 0.68 +0.6 87,610 - PGA1_c00890 0.72 +0.1 87,696 + PGA1_c00890 0.89 +1.2 87,704 - -0.6 87,715 + +0.8 87,715 + -1.7 87,715 + +0.6 87,792 + -2.9 87,803 + -1.2 87,803 + +0.9 87,803 + +0.8 87,805 + +1.1 87,812 + -2.6 88,719 + +0.1 88,719 - -0.7 88,727 + -2.0 88,727 - -2.6 88,758 - +0.2 88,760 + +1.0 88,876 + PGA1_c00910 0.58 +0.0 88,884 - PGA1_c00910 0.62 -0.7 89,068 - PGA1_c00920 0.12 -0.5 89,072 + PGA1_c00920 0.13 -0.1 89,078 - PGA1_c00920 0.14 -0.2 89,080 - PGA1_c00920 0.14 +0.5 89,082 + PGA1_c00920 0.14 -2.4 89,082 + PGA1_c00920 0.14 +0.6 89,082 + PGA1_c00920 0.14 +0.9 89,088 - PGA1_c00920 0.15 +0.6 89,116 - PGA1_c00920 0.20 -1.2 89,116 - PGA1_c00920 0.20 +1.0 89,139 - PGA1_c00920 0.24 +1.3 89,186 - PGA1_c00920 0.31 +0.2 89,188 + PGA1_c00920 0.31 -0.5 89,271 + PGA1_c00920 0.45 +0.9 89,271 + PGA1_c00920 0.45 +1.5 89,271 + PGA1_c00920 0.45 +1.7 89,279 - PGA1_c00920 0.46 -0.0 89,302 - PGA1_c00920 0.50 -0.5 89,306 - PGA1_c00920 0.50 -0.2 89,369 - PGA1_c00920 0.60 +0.0 89,445 - PGA1_c00920 0.73 -0.4 89,558 + -2.0 89,558 + -0.2 89,576 + +0.4 89,579 + -0.1 89,600 + -0.0 89,609 - -0.8 89,639 - -0.6 89,640 - -0.1 89,647 + -0.2
Or see this region's nucleotide sequence