Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08200 and PGA1_c08210 are separated by 154 nucleotides PGA1_c08210 and PGA1_c08220 are separated by 2 nucleotides PGA1_c08220 and PGA1_c08230 are separated by 39 nucleotides PGA1_c08230 and PGA1_c08240 are separated by 296 nucleotides
PGA1_c08200: PGA1_c08200 - cob(I)yrinic acid a,c-diamide adenosyltransferase CobO, at 850,002 to 850,637
_c08200
PGA1_c08210: PGA1_c08210 - Predicted enzyme related to lactoylglutathione lyase, at 850,792 to 851,199
_c08210
PGA1_c08220: PGA1_c08220 - Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily., at 851,202 to 851,579
_c08220
PGA1_c08230: PGA1_c08230 - Predicted transcriptional regulators, at 851,619 to 852,164
_c08230
PGA1_c08240: PGA1_c08240 - aerobic glycerol-3-phosphate dehydrogenase GlpD, at 852,461 to 854,050
_c08240
Position (kb) 851
852
853 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 850.631 kb on + strand at 850.631 kb on + strand at 850.631 kb on + strand at 850.639 kb on - strand at 850.639 kb on - strand at 850.639 kb on - strand at 850.639 kb on - strand at 850.718 kb on + strand at 850.718 kb on + strand at 850.718 kb on + strand at 850.718 kb on + strand at 850.731 kb on + strand at 850.815 kb on + strand at 850.818 kb on - strand at 850.827 kb on - strand at 850.847 kb on - strand, within PGA1_c08210 at 850.857 kb on - strand, within PGA1_c08210 at 850.857 kb on - strand, within PGA1_c08210 at 850.904 kb on + strand, within PGA1_c08210 at 850.930 kb on - strand, within PGA1_c08210 at 850.960 kb on + strand, within PGA1_c08210 at 850.969 kb on - strand, within PGA1_c08210 at 851.018 kb on - strand, within PGA1_c08210 at 851.031 kb on + strand, within PGA1_c08210 at 851.034 kb on - strand, within PGA1_c08210 at 851.036 kb on + strand, within PGA1_c08210 at 851.036 kb on + strand, within PGA1_c08210 at 851.036 kb on + strand, within PGA1_c08210 at 851.036 kb on + strand, within PGA1_c08210 at 851.036 kb on + strand, within PGA1_c08210 at 851.039 kb on - strand, within PGA1_c08210 at 851.039 kb on - strand, within PGA1_c08210 at 851.039 kb on - strand, within PGA1_c08210 at 851.039 kb on - strand, within PGA1_c08210 at 851.057 kb on + strand, within PGA1_c08210 at 851.065 kb on - strand, within PGA1_c08210 at 851.085 kb on - strand, within PGA1_c08210 at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.191 kb on - strand at 851.191 kb on - strand at 851.191 kb on - strand at 851.194 kb on + strand at 851.214 kb on + strand at 851.269 kb on + strand, within PGA1_c08220 at 851.274 kb on + strand, within PGA1_c08220 at 851.274 kb on + strand, within PGA1_c08220 at 851.282 kb on - strand, within PGA1_c08220 at 851.289 kb on - strand, within PGA1_c08220 at 851.324 kb on + strand, within PGA1_c08220 at 851.332 kb on - strand, within PGA1_c08220 at 851.371 kb on + strand, within PGA1_c08220 at 851.400 kb on - strand, within PGA1_c08220 at 851.444 kb on + strand, within PGA1_c08220 at 851.505 kb on - strand, within PGA1_c08220 at 851.505 kb on - strand, within PGA1_c08220 at 851.508 kb on + strand, within PGA1_c08220 at 851.515 kb on - strand, within PGA1_c08220 at 851.645 kb on + strand at 851.647 kb on + strand at 851.647 kb on + strand at 851.678 kb on - strand, within PGA1_c08230 at 851.699 kb on - strand, within PGA1_c08230 at 851.743 kb on - strand, within PGA1_c08230 at 851.766 kb on - strand, within PGA1_c08230 at 851.906 kb on + strand, within PGA1_c08230 at 851.935 kb on - strand, within PGA1_c08230 at 852.003 kb on - strand, within PGA1_c08230 at 852.043 kb on + strand, within PGA1_c08230 at 852.061 kb on + strand, within PGA1_c08230 at 852.110 kb on - strand at 852.112 kb on + strand at 852.112 kb on + strand at 852.112 kb on + strand at 852.113 kb on - strand at 852.113 kb on - strand at 852.120 kb on - strand at 852.120 kb on - strand at 852.120 kb on - strand at 852.120 kb on - strand at 852.135 kb on - strand at 852.160 kb on + strand at 852.190 kb on + strand at 852.251 kb on - strand at 852.368 kb on + strand at 852.385 kb on + strand at 852.443 kb on - strand at 852.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 850,631 + +1.6 850,631 + -0.7 850,631 + -0.4 850,639 - -0.4 850,639 - +1.6 850,639 - -0.2 850,639 - +1.2 850,718 + +0.9 850,718 + +0.0 850,718 + +0.6 850,718 + -0.7 850,731 + +0.6 850,815 + +1.6 850,818 - +1.6 850,827 - -1.4 850,847 - PGA1_c08210 0.13 -1.4 850,857 - PGA1_c08210 0.16 +0.6 850,857 - PGA1_c08210 0.16 +0.3 850,904 + PGA1_c08210 0.27 +0.0 850,930 - PGA1_c08210 0.34 -1.2 850,960 + PGA1_c08210 0.41 +0.6 850,969 - PGA1_c08210 0.43 +2.2 851,018 - PGA1_c08210 0.55 +0.3 851,031 + PGA1_c08210 0.59 +1.6 851,034 - PGA1_c08210 0.59 -0.7 851,036 + PGA1_c08210 0.60 -2.0 851,036 + PGA1_c08210 0.60 -0.4 851,036 + PGA1_c08210 0.60 +0.6 851,036 + PGA1_c08210 0.60 +1.3 851,036 + PGA1_c08210 0.60 -2.2 851,039 - PGA1_c08210 0.61 +0.0 851,039 - PGA1_c08210 0.61 +1.0 851,039 - PGA1_c08210 0.61 +0.0 851,039 - PGA1_c08210 0.61 -1.0 851,057 + PGA1_c08210 0.65 +0.0 851,065 - PGA1_c08210 0.67 +3.2 851,085 - PGA1_c08210 0.72 -0.7 851,183 + +0.4 851,183 + +0.0 851,183 + +0.0 851,191 - +1.6 851,191 - +1.6 851,191 - +0.0 851,194 + +1.6 851,214 + +0.0 851,269 + PGA1_c08220 0.18 +3.2 851,274 + PGA1_c08220 0.19 +1.0 851,274 + PGA1_c08220 0.19 +0.6 851,282 - PGA1_c08220 0.21 +2.6 851,289 - PGA1_c08220 0.23 +0.9 851,324 + PGA1_c08220 0.32 +1.6 851,332 - PGA1_c08220 0.34 -1.2 851,371 + PGA1_c08220 0.45 -1.0 851,400 - PGA1_c08220 0.52 -0.4 851,444 + PGA1_c08220 0.64 -0.1 851,505 - PGA1_c08220 0.80 -0.4 851,505 - PGA1_c08220 0.80 +0.0 851,508 + PGA1_c08220 0.81 -1.2 851,515 - PGA1_c08220 0.83 +2.6 851,645 + -1.4 851,647 + -1.1 851,647 + +0.0 851,678 - PGA1_c08230 0.11 +0.0 851,699 - PGA1_c08230 0.15 +0.0 851,743 - PGA1_c08230 0.23 +1.6 851,766 - PGA1_c08230 0.27 -0.7 851,906 + PGA1_c08230 0.53 -0.4 851,935 - PGA1_c08230 0.58 +2.6 852,003 - PGA1_c08230 0.70 +0.0 852,043 + PGA1_c08230 0.78 -1.2 852,061 + PGA1_c08230 0.81 -1.0 852,110 - +0.0 852,112 + +0.3 852,112 + +0.0 852,112 + -1.4 852,113 - +0.6 852,113 - +2.6 852,120 - -1.0 852,120 - +0.3 852,120 - +1.2 852,120 - -0.4 852,135 - +1.3 852,160 + +0.3 852,190 + +0.6 852,251 - +2.2 852,368 + +1.0 852,385 + +0.9 852,443 - -1.9 852,475 - -0.2
Or see this region's nucleotide sequence