Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08040 and PGA1_c08050 are separated by 75 nucleotides PGA1_c08050 and PGA1_c08060 are separated by 69 nucleotides PGA1_c08060 and PGA1_c08070 are separated by 432 nucleotides
PGA1_c08040: PGA1_c08040 - tRNA-Ser, at 834,204 to 834,293
_c08040
PGA1_c08050: PGA1_c08050 - putative precorrin-6A synthase (deacetylating), cobF, at 834,369 to 835,136
_c08050
PGA1_c08060: PGA1_c08060 - hypothetical protein, at 835,206 to 835,883
_c08060
PGA1_c08070: PGA1_c08070 - uroporphyrin-III C-methyltransferase CobA, at 836,316 to 837,080
_c08070
Position (kb) 835
836 Strain fitness (log2 ratio) -1
0
1 at 834.317 kb on - strand at 835.228 kb on + strand at 835.270 kb on + strand at 835.275 kb on + strand, within PGA1_c08060 at 835.275 kb on + strand, within PGA1_c08060 at 835.275 kb on + strand, within PGA1_c08060 at 835.278 kb on - strand, within PGA1_c08060 at 835.283 kb on - strand, within PGA1_c08060 at 835.327 kb on - strand, within PGA1_c08060 at 835.429 kb on - strand, within PGA1_c08060 at 835.489 kb on - strand, within PGA1_c08060 at 835.536 kb on + strand, within PGA1_c08060 at 835.593 kb on - strand, within PGA1_c08060 at 835.732 kb on + strand, within PGA1_c08060 at 835.740 kb on - strand, within PGA1_c08060 at 835.740 kb on - strand, within PGA1_c08060 at 835.740 kb on - strand, within PGA1_c08060 at 835.759 kb on - strand, within PGA1_c08060 at 835.759 kb on - strand, within PGA1_c08060 at 835.784 kb on + strand, within PGA1_c08060 at 835.809 kb on + strand, within PGA1_c08060 at 835.831 kb on + strand at 835.839 kb on - strand at 835.846 kb on + strand at 835.905 kb on + strand at 836.077 kb on + strand at 836.109 kb on + strand at 836.131 kb on - strand at 836.177 kb on + strand at 836.276 kb on + strand at 836.276 kb on + strand at 836.276 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 834,317 - -0.7 835,228 + +0.6 835,270 + -1.0 835,275 + PGA1_c08060 0.10 +0.6 835,275 + PGA1_c08060 0.10 -0.7 835,275 + PGA1_c08060 0.10 +0.6 835,278 - PGA1_c08060 0.11 -0.4 835,283 - PGA1_c08060 0.11 +0.6 835,327 - PGA1_c08060 0.18 +1.6 835,429 - PGA1_c08060 0.33 +0.6 835,489 - PGA1_c08060 0.42 +0.0 835,536 + PGA1_c08060 0.49 +0.0 835,593 - PGA1_c08060 0.57 -1.7 835,732 + PGA1_c08060 0.78 +0.0 835,740 - PGA1_c08060 0.79 +0.6 835,740 - PGA1_c08060 0.79 +1.0 835,740 - PGA1_c08060 0.79 +0.6 835,759 - PGA1_c08060 0.82 +0.0 835,759 - PGA1_c08060 0.82 +0.6 835,784 + PGA1_c08060 0.85 -0.2 835,809 + PGA1_c08060 0.89 +1.6 835,831 + +1.6 835,839 - -0.7 835,846 + +0.6 835,905 + -1.0 836,077 + -0.3 836,109 + -0.4 836,131 - -0.7 836,177 + +1.6 836,276 + +0.6 836,276 + +0.6 836,276 + +1.6
Or see this region's nucleotide sequence