Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06940 and PGA1_c06950 are separated by 186 nucleotides PGA1_c06950 and PGA1_c06960 are separated by 99 nucleotides PGA1_c06960 and PGA1_c06970 are separated by 256 nucleotides
PGA1_c06940: PGA1_c06940 - putative adenosylcobalamin(B12)-dependent ribonucleotide reductases, at 713,256 to 715,553
_c06940
PGA1_c06950: PGA1_c06950 - Uncharacterized conserved protein, at 715,740 to 716,171
_c06950
PGA1_c06960: PGA1_c06960 - Uncharacterized protein conserved in bacteria, at 716,271 to 716,588
_c06960
PGA1_c06970: PGA1_c06970 - pyruvate kinase PykA, at 716,845 to 718,290
_c06970
Position (kb)
715
716
717 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 714.740 kb on + strand, within PGA1_c06940 at 714.778 kb on + strand, within PGA1_c06940 at 714.854 kb on + strand, within PGA1_c06940 at 714.912 kb on + strand, within PGA1_c06940 at 714.917 kb on + strand, within PGA1_c06940 at 714.925 kb on - strand, within PGA1_c06940 at 714.976 kb on - strand, within PGA1_c06940 at 714.976 kb on - strand, within PGA1_c06940 at 715.017 kb on + strand, within PGA1_c06940 at 715.025 kb on - strand, within PGA1_c06940 at 715.153 kb on - strand, within PGA1_c06940 at 715.220 kb on + strand, within PGA1_c06940 at 715.249 kb on - strand, within PGA1_c06940 at 715.272 kb on + strand, within PGA1_c06940 at 715.280 kb on - strand, within PGA1_c06940 at 715.289 kb on + strand, within PGA1_c06940 at 715.292 kb on - strand, within PGA1_c06940 at 715.292 kb on - strand, within PGA1_c06940 at 715.292 kb on - strand, within PGA1_c06940 at 715.297 kb on - strand, within PGA1_c06940 at 715.307 kb on + strand, within PGA1_c06940 at 715.314 kb on - strand, within PGA1_c06940 at 715.315 kb on - strand, within PGA1_c06940 at 715.324 kb on + strand at 715.422 kb on - strand at 715.445 kb on + strand at 715.514 kb on + strand at 715.596 kb on + strand at 715.596 kb on + strand at 715.596 kb on + strand at 715.596 kb on + strand at 715.604 kb on - strand at 715.604 kb on - strand at 715.673 kb on - strand at 715.696 kb on - strand at 715.778 kb on + strand at 715.840 kb on - strand, within PGA1_c06950 at 715.840 kb on - strand, within PGA1_c06950 at 715.862 kb on + strand, within PGA1_c06950 at 715.862 kb on + strand, within PGA1_c06950 at 715.862 kb on + strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.870 kb on - strand, within PGA1_c06950 at 715.888 kb on - strand, within PGA1_c06950 at 715.889 kb on + strand, within PGA1_c06950 at 716.001 kb on + strand, within PGA1_c06950 at 716.039 kb on + strand, within PGA1_c06950 at 716.048 kb on + strand, within PGA1_c06950 at 716.056 kb on - strand, within PGA1_c06950 at 716.071 kb on + strand, within PGA1_c06950 at 716.086 kb on - strand, within PGA1_c06950 at 716.093 kb on - strand, within PGA1_c06950 at 716.104 kb on - strand, within PGA1_c06950 at 716.168 kb on - strand at 716.173 kb on - strand at 716.178 kb on - strand at 716.185 kb on + strand at 716.187 kb on + strand at 716.193 kb on - strand at 716.205 kb on + strand at 716.205 kb on + strand at 716.213 kb on - strand at 716.242 kb on + strand at 716.366 kb on + strand, within PGA1_c06960 at 716.388 kb on - strand, within PGA1_c06960 at 716.390 kb on + strand, within PGA1_c06960 at 716.441 kb on + strand, within PGA1_c06960 at 716.449 kb on - strand, within PGA1_c06960 at 716.449 kb on - strand, within PGA1_c06960 at 716.449 kb on - strand, within PGA1_c06960 at 716.631 kb on + strand at 716.631 kb on + strand at 716.631 kb on + strand at 716.631 kb on + strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.639 kb on - strand at 716.641 kb on + strand at 716.644 kb on - strand at 716.661 kb on + strand at 716.695 kb on - strand at 716.717 kb on + strand at 716.750 kb on + strand at 716.752 kb on - strand at 716.835 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 714,740 + PGA1_c06940 0.65 -0.6 714,778 + PGA1_c06940 0.66 +1.0 714,854 + PGA1_c06940 0.70 +1.7 714,912 + PGA1_c06940 0.72 +0.3 714,917 + PGA1_c06940 0.72 -0.2 714,925 - PGA1_c06940 0.73 -0.1 714,976 - PGA1_c06940 0.75 +0.2 714,976 - PGA1_c06940 0.75 +0.6 715,017 + PGA1_c06940 0.77 -1.2 715,025 - PGA1_c06940 0.77 -1.2 715,153 - PGA1_c06940 0.83 -0.2 715,220 + PGA1_c06940 0.85 -0.3 715,249 - PGA1_c06940 0.87 +0.9 715,272 + PGA1_c06940 0.88 -1.6 715,280 - PGA1_c06940 0.88 +0.4 715,289 + PGA1_c06940 0.88 +0.1 715,292 - PGA1_c06940 0.89 -1.8 715,292 - PGA1_c06940 0.89 +0.7 715,292 - PGA1_c06940 0.89 +1.1 715,297 - PGA1_c06940 0.89 +0.7 715,307 + PGA1_c06940 0.89 -0.8 715,314 - PGA1_c06940 0.90 -0.9 715,315 - PGA1_c06940 0.90 +0.9 715,324 + +1.1 715,422 - -1.6 715,445 + -1.3 715,514 + +1.3 715,596 + +0.1 715,596 + -2.2 715,596 + -1.8 715,596 + +0.5 715,604 - +1.7 715,604 - -0.9 715,673 - -0.9 715,696 - -0.2 715,778 + +0.1 715,840 - PGA1_c06950 0.23 +0.8 715,840 - PGA1_c06950 0.23 -3.0 715,862 + PGA1_c06950 0.28 +0.7 715,862 + PGA1_c06950 0.28 +0.9 715,862 + PGA1_c06950 0.28 -0.3 715,870 - PGA1_c06950 0.30 -0.0 715,870 - PGA1_c06950 0.30 -0.6 715,870 - PGA1_c06950 0.30 -1.8 715,870 - PGA1_c06950 0.30 -1.3 715,888 - PGA1_c06950 0.34 +1.2 715,889 + PGA1_c06950 0.34 +1.7 716,001 + PGA1_c06950 0.60 -0.3 716,039 + PGA1_c06950 0.69 -0.0 716,048 + PGA1_c06950 0.71 -0.6 716,056 - PGA1_c06950 0.73 -0.9 716,071 + PGA1_c06950 0.77 +0.9 716,086 - PGA1_c06950 0.80 +0.1 716,093 - PGA1_c06950 0.82 -0.8 716,104 - PGA1_c06950 0.84 +0.5 716,168 - -0.3 716,173 - +0.4 716,178 - +0.9 716,185 + -1.2 716,187 + -0.3 716,193 - -0.9 716,205 + +0.7 716,205 + +0.8 716,213 - -0.5 716,242 + +0.9 716,366 + PGA1_c06960 0.30 -1.3 716,388 - PGA1_c06960 0.37 -0.9 716,390 + PGA1_c06960 0.37 -1.3 716,441 + PGA1_c06960 0.53 +0.5 716,449 - PGA1_c06960 0.56 +0.5 716,449 - PGA1_c06960 0.56 -0.9 716,449 - PGA1_c06960 0.56 -1.4 716,631 + -0.8 716,631 + +1.1 716,631 + +0.9 716,631 + -0.8 716,639 - -2.1 716,639 - -0.1 716,639 - -0.6 716,639 - -0.2 716,639 - +1.3 716,639 - -0.3 716,639 - +0.5 716,639 - -0.5 716,641 + -0.2 716,644 - +0.7 716,661 + +0.0 716,695 - -0.3 716,717 + +0.2 716,750 + -0.3 716,752 - -1.5 716,835 + +0.1
Or see this region's nucleotide sequence