Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06470 and PGA1_c06480 are separated by 33 nucleotides PGA1_c06480 and PGA1_c06490 are separated by 121 nucleotides PGA1_c06490 and PGA1_c06500 are separated by 9 nucleotides
PGA1_c06470: PGA1_c06470 - glycyl-tRNA synthetase alpha subunit, at 662,267 to 663,196
_c06470
PGA1_c06480: PGA1_c06480 - hypothetical protein, at 663,230 to 663,763
_c06480
PGA1_c06490: PGA1_c06490 - sodium/glutamate symport carrier protein GltS, at 663,885 to 665,093
_c06490
PGA1_c06500: PGA1_c06500 - glycyl-tRNA synthetase beta subunit, at 665,103 to 667,367
_c06500
Position (kb)
663
664
665
666 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 663.191 kb on + strand at 663.227 kb on + strand at 663.302 kb on - strand, within PGA1_c06480 at 663.698 kb on + strand, within PGA1_c06480 at 663.698 kb on + strand, within PGA1_c06480 at 663.729 kb on + strand at 663.757 kb on - strand at 663.771 kb on + strand at 663.801 kb on - strand at 663.834 kb on - strand at 663.861 kb on + strand at 663.861 kb on + strand at 663.861 kb on + strand at 663.861 kb on + strand at 663.869 kb on - strand at 663.896 kb on + strand at 663.896 kb on + strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.904 kb on - strand at 663.966 kb on - strand at 663.978 kb on + strand at 663.978 kb on + strand at 664.046 kb on + strand, within PGA1_c06490 at 664.054 kb on + strand, within PGA1_c06490 at 664.062 kb on - strand, within PGA1_c06490 at 664.285 kb on - strand, within PGA1_c06490 at 664.303 kb on - strand, within PGA1_c06490 at 664.350 kb on + strand, within PGA1_c06490 at 664.390 kb on + strand, within PGA1_c06490 at 664.557 kb on - strand, within PGA1_c06490 at 664.605 kb on - strand, within PGA1_c06490 at 664.605 kb on - strand, within PGA1_c06490 at 664.725 kb on - strand, within PGA1_c06490 at 664.787 kb on + strand, within PGA1_c06490 at 664.797 kb on + strand, within PGA1_c06490 at 664.809 kb on + strand, within PGA1_c06490 at 664.809 kb on + strand, within PGA1_c06490 at 664.812 kb on - strand, within PGA1_c06490 at 664.843 kb on - strand, within PGA1_c06490 at 664.843 kb on - strand, within PGA1_c06490 at 664.843 kb on - strand, within PGA1_c06490 at 664.866 kb on - strand, within PGA1_c06490 at 664.933 kb on + strand, within PGA1_c06490 at 664.941 kb on - strand, within PGA1_c06490 at 664.941 kb on - strand, within PGA1_c06490 at 664.985 kb on - strand at 664.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 663,191 + -0.8 663,227 + -0.2 663,302 - PGA1_c06480 0.13 -0.7 663,698 + PGA1_c06480 0.88 -1.6 663,698 + PGA1_c06480 0.88 +1.1 663,729 + +0.7 663,757 - -1.7 663,771 + -1.2 663,801 - +0.3 663,834 - +1.1 663,861 + +1.1 663,861 + -0.7 663,861 + +0.2 663,861 + -0.3 663,869 - +0.1 663,896 + -0.5 663,896 + +0.4 663,904 - +0.9 663,904 - -0.2 663,904 - -2.2 663,904 - +0.3 663,904 - +1.0 663,966 - +0.7 663,978 + -1.1 663,978 + -0.4 664,046 + PGA1_c06490 0.13 +2.1 664,054 + PGA1_c06490 0.14 -0.2 664,062 - PGA1_c06490 0.15 -0.0 664,285 - PGA1_c06490 0.33 -2.2 664,303 - PGA1_c06490 0.35 +0.3 664,350 + PGA1_c06490 0.38 -0.3 664,390 + PGA1_c06490 0.42 +0.1 664,557 - PGA1_c06490 0.56 +0.8 664,605 - PGA1_c06490 0.60 -1.2 664,605 - PGA1_c06490 0.60 -0.5 664,725 - PGA1_c06490 0.69 +0.7 664,787 + PGA1_c06490 0.75 +0.1 664,797 + PGA1_c06490 0.75 -0.5 664,809 + PGA1_c06490 0.76 +0.4 664,809 + PGA1_c06490 0.76 -0.9 664,812 - PGA1_c06490 0.77 +1.5 664,843 - PGA1_c06490 0.79 +0.1 664,843 - PGA1_c06490 0.79 -1.4 664,843 - PGA1_c06490 0.79 +0.1 664,866 - PGA1_c06490 0.81 -0.1 664,933 + PGA1_c06490 0.87 -0.5 664,941 - PGA1_c06490 0.87 +1.2 664,941 - PGA1_c06490 0.87 +1.7 664,985 - -0.6 664,990 + -0.5
Or see this region's nucleotide sequence