Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00040 and PGA1_c00050 are separated by 14 nucleotides PGA1_c00050 and PGA1_c00060 are separated by 100 nucleotides PGA1_c00060 and PGA1_c00070 are separated by 85 nucleotides
PGA1_c00040: PGA1_c00040 - putative LysE type translocator, at 4,151 to 4,771
_c00040
PGA1_c00050: PGA1_c00050 - putative transcriptional regulator, tetR family, at 4,786 to 5,385
_c00050
PGA1_c00060: PGA1_c00060 - putative NAD(P)H dehydrogenase (quinone), at 5,486 to 6,079
_c00060
PGA1_c00070: PGA1_c00070 - DNA gyrase subunit GyrB, at 6,165 to 8,582
_c00070
Position (kb) 5
6
7 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4.522 kb on - strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.581 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.666 kb on - strand, within PGA1_c00040 at 4.671 kb on - strand, within PGA1_c00040 at 4.777 kb on + strand at 4.780 kb on - strand at 4.780 kb on - strand at 4.785 kb on + strand at 4.825 kb on + strand at 4.858 kb on + strand, within PGA1_c00050 at 4.873 kb on + strand, within PGA1_c00050 at 4.913 kb on + strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.950 kb on + strand, within PGA1_c00050 at 4.971 kb on + strand, within PGA1_c00050 at 4.979 kb on - strand, within PGA1_c00050 at 5.031 kb on - strand, within PGA1_c00050 at 5.057 kb on - strand, within PGA1_c00050 at 5.068 kb on - strand, within PGA1_c00050 at 5.103 kb on + strand, within PGA1_c00050 at 5.148 kb on - strand, within PGA1_c00050 at 5.152 kb on + strand, within PGA1_c00050 at 5.304 kb on - strand, within PGA1_c00050 at 5.304 kb on - strand, within PGA1_c00050 at 5.367 kb on + strand at 5.367 kb on + strand at 5.373 kb on - strand at 5.373 kb on - strand at 5.375 kb on - strand at 5.409 kb on - strand at 5.472 kb on - strand at 5.480 kb on + strand at 5.482 kb on + strand at 5.490 kb on - strand at 5.490 kb on - strand at 5.525 kb on + strand at 5.578 kb on + strand, within PGA1_c00060 at 5.588 kb on + strand, within PGA1_c00060 at 5.594 kb on - strand, within PGA1_c00060 at 5.594 kb on - strand, within PGA1_c00060 at 5.601 kb on + strand, within PGA1_c00060 at 5.601 kb on + strand, within PGA1_c00060 at 5.707 kb on - strand, within PGA1_c00060 at 5.707 kb on - strand, within PGA1_c00060 at 5.741 kb on - strand, within PGA1_c00060 at 5.743 kb on - strand, within PGA1_c00060 at 5.764 kb on + strand, within PGA1_c00060 at 5.772 kb on - strand, within PGA1_c00060 at 5.876 kb on + strand, within PGA1_c00060 at 5.883 kb on + strand, within PGA1_c00060 at 5.883 kb on + strand, within PGA1_c00060 at 5.892 kb on + strand, within PGA1_c00060 at 5.894 kb on + strand, within PGA1_c00060 at 5.894 kb on + strand, within PGA1_c00060 at 5.904 kb on + strand, within PGA1_c00060 at 5.914 kb on + strand, within PGA1_c00060 at 5.949 kb on + strand, within PGA1_c00060 at 5.978 kb on - strand, within PGA1_c00060 at 5.994 kb on + strand, within PGA1_c00060 at 6.029 kb on + strand at 6.043 kb on + strand at 6.050 kb on + strand at 6.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 4,522 - PGA1_c00040 0.60 -0.1 4,580 + PGA1_c00040 0.69 +0.5 4,580 + PGA1_c00040 0.69 +0.2 4,581 - PGA1_c00040 0.69 -1.2 4,656 - PGA1_c00040 0.81 -0.5 4,656 - PGA1_c00040 0.81 -1.0 4,666 - PGA1_c00040 0.83 +1.6 4,671 - PGA1_c00040 0.84 +0.0 4,777 + -1.8 4,780 - +0.2 4,780 - +0.2 4,785 + -0.4 4,825 + +0.8 4,858 + PGA1_c00050 0.12 +0.1 4,873 + PGA1_c00050 0.14 +1.1 4,913 + PGA1_c00050 0.21 -0.4 4,921 - PGA1_c00050 0.23 -2.4 4,921 - PGA1_c00050 0.23 -0.1 4,950 + PGA1_c00050 0.27 +0.8 4,971 + PGA1_c00050 0.31 -2.5 4,979 - PGA1_c00050 0.32 -0.6 5,031 - PGA1_c00050 0.41 +0.2 5,057 - PGA1_c00050 0.45 -0.4 5,068 - PGA1_c00050 0.47 +3.3 5,103 + PGA1_c00050 0.53 +0.0 5,148 - PGA1_c00050 0.60 -0.0 5,152 + PGA1_c00050 0.61 +0.8 5,304 - PGA1_c00050 0.86 +0.0 5,304 - PGA1_c00050 0.86 -0.6 5,367 + -0.4 5,367 + -1.7 5,373 - -3.8 5,373 - -1.3 5,375 - +0.2 5,409 - +0.1 5,472 - -0.2 5,480 + +0.2 5,482 + -0.5 5,490 - -1.6 5,490 - +0.2 5,525 + +0.9 5,578 + PGA1_c00060 0.15 +1.6 5,588 + PGA1_c00060 0.17 -1.8 5,594 - PGA1_c00060 0.18 +1.3 5,594 - PGA1_c00060 0.18 +1.2 5,601 + PGA1_c00060 0.19 +0.8 5,601 + PGA1_c00060 0.19 +0.8 5,707 - PGA1_c00060 0.37 -0.4 5,707 - PGA1_c00060 0.37 +0.4 5,741 - PGA1_c00060 0.43 +2.3 5,743 - PGA1_c00060 0.43 +0.9 5,764 + PGA1_c00060 0.47 -0.0 5,772 - PGA1_c00060 0.48 +0.3 5,876 + PGA1_c00060 0.66 -0.3 5,883 + PGA1_c00060 0.67 +0.0 5,883 + PGA1_c00060 0.67 +0.3 5,892 + PGA1_c00060 0.68 +0.4 5,894 + PGA1_c00060 0.69 +0.6 5,894 + PGA1_c00060 0.69 +0.4 5,904 + PGA1_c00060 0.70 +0.5 5,914 + PGA1_c00060 0.72 -1.0 5,949 + PGA1_c00060 0.78 -1.8 5,978 - PGA1_c00060 0.83 -1.1 5,994 + PGA1_c00060 0.86 -1.6 6,029 + -2.5 6,043 + -0.2 6,050 + -2.3 6,051 - -0.8
Or see this region's nucleotide sequence