Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05540 and PGA1_c05550 are separated by 342 nucleotides PGA1_c05550 and PGA1_c05560 are separated by 623 nucleotides
PGA1_c05540: PGA1_c05540 - transcriptional regulator, IclR family, at 565,964 to 566,782
_c05540
PGA1_c05550: PGA1_c05550 - glutamine synthase-like protein, at 567,125 to 568,576
_c05550
PGA1_c05560: PGA1_c05560 - glycine cleavage system protein GcvH, at 569,200 to 569,562
_c05560
Position (kb) 567
568
569 Strain fitness (log2 ratio) -2
-1
0
1
2 at 566.126 kb on + strand, within PGA1_c05540 at 566.146 kb on + strand, within PGA1_c05540 at 566.156 kb on - strand, within PGA1_c05540 at 566.158 kb on + strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.173 kb on - strand, within PGA1_c05540 at 566.181 kb on + strand, within PGA1_c05540 at 566.238 kb on + strand, within PGA1_c05540 at 566.296 kb on + strand, within PGA1_c05540 at 566.312 kb on + strand, within PGA1_c05540 at 566.312 kb on + strand, within PGA1_c05540 at 566.313 kb on + strand, within PGA1_c05540 at 566.400 kb on - strand, within PGA1_c05540 at 566.410 kb on - strand, within PGA1_c05540 at 566.434 kb on + strand, within PGA1_c05540 at 566.444 kb on - strand, within PGA1_c05540 at 566.456 kb on + strand, within PGA1_c05540 at 566.473 kb on - strand, within PGA1_c05540 at 566.513 kb on - strand, within PGA1_c05540 at 566.515 kb on + strand, within PGA1_c05540 at 566.532 kb on - strand, within PGA1_c05540 at 566.550 kb on - strand, within PGA1_c05540 at 566.606 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.615 kb on - strand, within PGA1_c05540 at 566.615 kb on - strand, within PGA1_c05540 at 566.620 kb on - strand, within PGA1_c05540 at 566.644 kb on + strand, within PGA1_c05540 at 566.650 kb on + strand, within PGA1_c05540 at 566.675 kb on + strand, within PGA1_c05540 at 566.770 kb on - strand at 566.799 kb on + strand at 566.800 kb on + strand at 566.800 kb on + strand at 566.808 kb on - strand at 566.830 kb on + strand at 566.831 kb on + strand at 566.831 kb on + strand at 566.831 kb on + strand at 566.831 kb on + strand at 566.882 kb on + strand at 566.882 kb on + strand at 566.890 kb on - strand at 566.895 kb on - strand at 567.052 kb on - strand at 567.057 kb on - strand at 567.059 kb on - strand at 567.063 kb on + strand at 567.065 kb on + strand at 567.142 kb on - strand at 567.176 kb on + strand at 567.189 kb on - strand at 567.192 kb on + strand at 567.214 kb on + strand at 567.214 kb on + strand at 567.222 kb on - strand at 567.229 kb on + strand at 567.237 kb on + strand at 567.237 kb on - strand at 567.285 kb on - strand, within PGA1_c05550 at 567.299 kb on + strand, within PGA1_c05550 at 567.302 kb on - strand, within PGA1_c05550 at 567.354 kb on - strand, within PGA1_c05550 at 567.414 kb on + strand, within PGA1_c05550 at 567.475 kb on + strand, within PGA1_c05550 at 567.475 kb on + strand, within PGA1_c05550 at 567.475 kb on + strand, within PGA1_c05550 at 567.488 kb on + strand, within PGA1_c05550 at 567.536 kb on - strand, within PGA1_c05550 at 567.565 kb on - strand, within PGA1_c05550 at 567.653 kb on + strand, within PGA1_c05550 at 567.653 kb on + strand, within PGA1_c05550 at 567.656 kb on - strand, within PGA1_c05550 at 567.699 kb on + strand, within PGA1_c05550 at 567.710 kb on + strand, within PGA1_c05550 at 567.739 kb on + strand, within PGA1_c05550 at 567.751 kb on + strand, within PGA1_c05550 at 567.917 kb on - strand, within PGA1_c05550 at 567.920 kb on - strand, within PGA1_c05550 at 567.927 kb on - strand, within PGA1_c05550 at 567.961 kb on + strand, within PGA1_c05550 at 567.961 kb on + strand, within PGA1_c05550 at 567.978 kb on - strand, within PGA1_c05550 at 567.979 kb on - strand, within PGA1_c05550 at 568.020 kb on - strand, within PGA1_c05550 at 568.070 kb on + strand, within PGA1_c05550 at 568.083 kb on - strand, within PGA1_c05550 at 568.108 kb on + strand, within PGA1_c05550 at 568.139 kb on + strand, within PGA1_c05550 at 568.147 kb on - strand, within PGA1_c05550 at 568.147 kb on - strand, within PGA1_c05550 at 568.242 kb on - strand, within PGA1_c05550 at 568.277 kb on + strand, within PGA1_c05550 at 568.321 kb on - strand, within PGA1_c05550 at 568.321 kb on - strand, within PGA1_c05550 at 568.418 kb on + strand, within PGA1_c05550 at 568.440 kb on - strand at 568.446 kb on - strand at 568.464 kb on + strand at 568.466 kb on + strand at 568.474 kb on + strand at 568.487 kb on + strand at 568.496 kb on - strand at 568.501 kb on + strand at 568.504 kb on - strand at 568.507 kb on + strand at 568.515 kb on - strand at 568.515 kb on - strand at 568.533 kb on + strand at 568.541 kb on - strand at 568.776 kb on + strand at 568.783 kb on + strand at 568.790 kb on - strand at 568.808 kb on + strand at 568.829 kb on + strand at 568.837 kb on - strand at 568.845 kb on + strand at 568.874 kb on - strand at 568.892 kb on + strand at 568.892 kb on + strand at 568.900 kb on - strand at 568.931 kb on + strand at 568.999 kb on - strand at 569.044 kb on + strand at 569.044 kb on + strand at 569.044 kb on + strand at 569.044 kb on + strand at 569.049 kb on + strand at 569.049 kb on + strand at 569.052 kb on - strand at 569.054 kb on + strand at 569.057 kb on - strand at 569.068 kb on + strand at 569.073 kb on + strand at 569.076 kb on - strand at 569.081 kb on - strand at 569.147 kb on + strand at 569.324 kb on + strand, within PGA1_c05560 at 569.366 kb on - strand, within PGA1_c05560 at 569.375 kb on - strand, within PGA1_c05560 at 569.469 kb on - strand, within PGA1_c05560 at 569.490 kb on + strand, within PGA1_c05560 at 569.498 kb on - strand, within PGA1_c05560 at 569.506 kb on - strand, within PGA1_c05560 at 569.573 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 566,126 + PGA1_c05540 0.20 -0.2 566,146 + PGA1_c05540 0.22 +0.8 566,156 - PGA1_c05540 0.23 +0.1 566,158 + PGA1_c05540 0.24 +0.5 566,166 - PGA1_c05540 0.25 -1.1 566,166 - PGA1_c05540 0.25 -0.1 566,166 - PGA1_c05540 0.25 +0.7 566,166 - PGA1_c05540 0.25 -0.1 566,166 - PGA1_c05540 0.25 +2.7 566,166 - PGA1_c05540 0.25 -0.3 566,173 - PGA1_c05540 0.26 +1.7 566,181 + PGA1_c05540 0.26 +0.1 566,238 + PGA1_c05540 0.33 +0.2 566,296 + PGA1_c05540 0.41 +0.2 566,312 + PGA1_c05540 0.42 -0.0 566,312 + PGA1_c05540 0.42 -2.1 566,313 + PGA1_c05540 0.43 +0.1 566,400 - PGA1_c05540 0.53 -0.3 566,410 - PGA1_c05540 0.54 +1.1 566,434 + PGA1_c05540 0.57 -1.3 566,444 - PGA1_c05540 0.59 -0.7 566,456 + PGA1_c05540 0.60 -1.5 566,473 - PGA1_c05540 0.62 -1.8 566,513 - PGA1_c05540 0.67 +1.7 566,515 + PGA1_c05540 0.67 -0.1 566,532 - PGA1_c05540 0.69 -0.3 566,550 - PGA1_c05540 0.72 -1.1 566,606 + PGA1_c05540 0.78 +1.9 566,612 + PGA1_c05540 0.79 +2.1 566,612 + PGA1_c05540 0.79 +1.2 566,612 + PGA1_c05540 0.79 +1.5 566,612 + PGA1_c05540 0.79 -1.3 566,612 + PGA1_c05540 0.79 +0.1 566,612 + PGA1_c05540 0.79 -0.1 566,612 + PGA1_c05540 0.79 +0.9 566,612 + PGA1_c05540 0.79 +1.7 566,615 - PGA1_c05540 0.79 -0.7 566,615 - PGA1_c05540 0.79 +1.1 566,620 - PGA1_c05540 0.80 -0.5 566,644 + PGA1_c05540 0.83 -0.1 566,650 + PGA1_c05540 0.84 +0.4 566,675 + PGA1_c05540 0.87 +0.7 566,770 - -1.1 566,799 + +1.7 566,800 + +1.7 566,800 + -1.8 566,808 - -0.9 566,830 + +0.7 566,831 + +1.7 566,831 + -1.3 566,831 + -0.3 566,831 + -0.3 566,882 + +1.7 566,882 + -1.3 566,890 - +1.1 566,895 - +1.1 567,052 - -1.5 567,057 - -0.6 567,059 - +0.2 567,063 + +0.7 567,065 + -0.3 567,142 - -0.3 567,176 + +1.7 567,189 - +1.3 567,192 + -0.9 567,214 + -0.5 567,214 + -0.2 567,222 - +1.2 567,229 + -0.3 567,237 + -2.0 567,237 - +0.8 567,285 - PGA1_c05550 0.11 +0.1 567,299 + PGA1_c05550 0.12 -0.3 567,302 - PGA1_c05550 0.12 -0.1 567,354 - PGA1_c05550 0.16 +0.5 567,414 + PGA1_c05550 0.20 +0.1 567,475 + PGA1_c05550 0.24 -0.1 567,475 + PGA1_c05550 0.24 +0.1 567,475 + PGA1_c05550 0.24 +1.1 567,488 + PGA1_c05550 0.25 -1.2 567,536 - PGA1_c05550 0.28 +0.9 567,565 - PGA1_c05550 0.30 +0.3 567,653 + PGA1_c05550 0.36 -0.3 567,653 + PGA1_c05550 0.36 -0.3 567,656 - PGA1_c05550 0.37 +0.1 567,699 + PGA1_c05550 0.40 +0.2 567,710 + PGA1_c05550 0.40 +0.1 567,739 + PGA1_c05550 0.42 -0.9 567,751 + PGA1_c05550 0.43 +0.4 567,917 - PGA1_c05550 0.55 +0.1 567,920 - PGA1_c05550 0.55 +1.2 567,927 - PGA1_c05550 0.55 -0.3 567,961 + PGA1_c05550 0.58 +1.7 567,961 + PGA1_c05550 0.58 +0.7 567,978 - PGA1_c05550 0.59 +0.7 567,979 - PGA1_c05550 0.59 +1.1 568,020 - PGA1_c05550 0.62 -0.3 568,070 + PGA1_c05550 0.65 +0.4 568,083 - PGA1_c05550 0.66 +0.7 568,108 + PGA1_c05550 0.68 -0.2 568,139 + PGA1_c05550 0.70 +1.1 568,147 - PGA1_c05550 0.70 +0.2 568,147 - PGA1_c05550 0.70 -0.9 568,242 - PGA1_c05550 0.77 -0.3 568,277 + PGA1_c05550 0.79 +0.3 568,321 - PGA1_c05550 0.82 +0.7 568,321 - PGA1_c05550 0.82 +0.7 568,418 + PGA1_c05550 0.89 +0.3 568,440 - -1.3 568,446 - +1.4 568,464 + +2.1 568,466 + -0.5 568,474 + -0.5 568,487 + +2.2 568,496 - -0.3 568,501 + -0.7 568,504 - -0.6 568,507 + +0.7 568,515 - +0.1 568,515 - +1.7 568,533 + -0.7 568,541 - -0.2 568,776 + +0.7 568,783 + +0.1 568,790 - -1.5 568,808 + -0.2 568,829 + +0.7 568,837 - -0.7 568,845 + -0.3 568,874 - +1.1 568,892 + +0.7 568,892 + -0.1 568,900 - -0.9 568,931 + -0.7 568,999 - -1.8 569,044 + -0.5 569,044 + -1.5 569,044 + +2.1 569,044 + -0.3 569,049 + +1.1 569,049 + -0.2 569,052 - -0.5 569,054 + -1.9 569,057 - +0.7 569,068 + +0.1 569,073 + +1.0 569,076 - +0.7 569,081 - +0.3 569,147 + +1.1 569,324 + PGA1_c05560 0.34 +1.3 569,366 - PGA1_c05560 0.46 -0.7 569,375 - PGA1_c05560 0.48 +0.1 569,469 - PGA1_c05560 0.74 -0.9 569,490 + PGA1_c05560 0.80 +1.2 569,498 - PGA1_c05560 0.82 +1.1 569,506 - PGA1_c05560 0.84 +0.9 569,573 - +0.3
Or see this region's nucleotide sequence