Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04870 and PGA1_c04880 are separated by 77 nucleotides PGA1_c04880 and PGA1_c04890 are separated by 9 nucleotides
PGA1_c04870: PGA1_c04870 - tryptophan synthase beta chain, at 478,592 to 479,845
_c04870
PGA1_c04880: PGA1_c04880 - Uncharacterized protein conserved in bacteria, at 479,923 to 480,306
_c04880
PGA1_c04890: PGA1_c04890 - putative beta-lactamase, at 480,316 to 481,479
_c04890
Position (kb)
479
480
481 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 479.813 kb on - strand at 479.844 kb on + strand at 479.852 kb on - strand at 479.852 kb on - strand at 479.904 kb on + strand at 479.914 kb on + strand at 479.921 kb on + strand at 479.929 kb on - strand at 479.929 kb on - strand at 479.938 kb on - strand at 479.951 kb on - strand at 479.963 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.038 kb on - strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.073 kb on + strand, within PGA1_c04880 at 480.073 kb on + strand, within PGA1_c04880 at 480.097 kb on + strand, within PGA1_c04880 at 480.119 kb on + strand, within PGA1_c04880 at 480.131 kb on - strand, within PGA1_c04880 at 480.173 kb on + strand, within PGA1_c04880 at 480.180 kb on + strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.191 kb on + strand, within PGA1_c04880 at 480.198 kb on - strand, within PGA1_c04880 at 480.198 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.232 kb on - strand, within PGA1_c04880 at 480.267 kb on + strand, within PGA1_c04880 at 480.270 kb on - strand at 480.282 kb on + strand at 480.290 kb on - strand at 480.295 kb on + strand at 480.308 kb on + strand at 480.313 kb on + strand at 480.323 kb on + strand at 480.392 kb on + strand at 480.397 kb on + strand at 480.400 kb on - strand at 480.405 kb on - strand at 480.426 kb on + strand at 480.438 kb on - strand, within PGA1_c04890 at 480.455 kb on + strand, within PGA1_c04890 at 480.463 kb on - strand, within PGA1_c04890 at 480.516 kb on - strand, within PGA1_c04890 at 480.540 kb on - strand, within PGA1_c04890 at 480.550 kb on + strand, within PGA1_c04890 at 480.558 kb on - strand, within PGA1_c04890 at 480.583 kb on - strand, within PGA1_c04890 at 480.593 kb on + strand, within PGA1_c04890 at 480.661 kb on + strand, within PGA1_c04890 at 480.666 kb on + strand, within PGA1_c04890 at 480.678 kb on + strand, within PGA1_c04890 at 480.681 kb on - strand, within PGA1_c04890 at 480.692 kb on + strand, within PGA1_c04890 at 480.696 kb on + strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.891 kb on + strand, within PGA1_c04890 at 480.898 kb on + strand, within PGA1_c04890 at 480.899 kb on - strand, within PGA1_c04890 at 480.906 kb on - strand, within PGA1_c04890 at 480.934 kb on + strand, within PGA1_c04890 at 480.970 kb on + strand, within PGA1_c04890 at 480.978 kb on - strand, within PGA1_c04890 at 480.978 kb on - strand, within PGA1_c04890 at 481.007 kb on + strand, within PGA1_c04890 at 481.079 kb on + strand, within PGA1_c04890 at 481.142 kb on + strand, within PGA1_c04890 at 481.199 kb on - strand, within PGA1_c04890 at 481.249 kb on + strand, within PGA1_c04890 at 481.249 kb on + strand, within PGA1_c04890 at 481.259 kb on + strand, within PGA1_c04890 at 481.274 kb on - strand, within PGA1_c04890 at 481.274 kb on - strand, within PGA1_c04890 at 481.284 kb on - strand, within PGA1_c04890 at 481.284 kb on - strand, within PGA1_c04890
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 479,813 - -1.0 479,844 + -0.7 479,852 - -0.4 479,852 - -0.3 479,904 + -1.1 479,914 + +0.5 479,921 + -1.1 479,929 - -0.7 479,929 - -2.0 479,938 - -0.9 479,951 - +0.5 479,963 - PGA1_c04880 0.10 -1.3 480,033 - PGA1_c04880 0.29 +0.1 480,033 - PGA1_c04880 0.29 +0.3 480,033 - PGA1_c04880 0.29 -1.0 480,038 - PGA1_c04880 0.30 +0.5 480,063 + PGA1_c04880 0.36 -1.6 480,063 + PGA1_c04880 0.36 +0.1 480,063 + PGA1_c04880 0.36 -0.4 480,063 + PGA1_c04880 0.36 -0.3 480,073 + PGA1_c04880 0.39 -0.1 480,073 + PGA1_c04880 0.39 -1.9 480,097 + PGA1_c04880 0.45 -0.4 480,119 + PGA1_c04880 0.51 +0.4 480,131 - PGA1_c04880 0.54 -0.2 480,173 + PGA1_c04880 0.65 -0.6 480,180 + PGA1_c04880 0.67 -0.5 480,188 - PGA1_c04880 0.69 +0.3 480,188 - PGA1_c04880 0.69 -0.2 480,188 - PGA1_c04880 0.69 -0.2 480,191 + PGA1_c04880 0.70 -1.3 480,198 - PGA1_c04880 0.72 -0.4 480,198 - PGA1_c04880 0.72 +0.5 480,199 - PGA1_c04880 0.72 -1.5 480,199 - PGA1_c04880 0.72 -2.2 480,199 - PGA1_c04880 0.72 -0.7 480,232 - PGA1_c04880 0.80 -0.3 480,267 + PGA1_c04880 0.90 -1.0 480,270 - +0.6 480,282 + -0.9 480,290 - -1.3 480,295 + +0.4 480,308 + -0.6 480,313 + -0.9 480,323 + +0.1 480,392 + +0.3 480,397 + +1.0 480,400 - +0.5 480,405 - +0.2 480,426 + +1.1 480,438 - PGA1_c04890 0.10 -2.2 480,455 + PGA1_c04890 0.12 +0.6 480,463 - PGA1_c04890 0.13 +1.0 480,516 - PGA1_c04890 0.17 -0.2 480,540 - PGA1_c04890 0.19 +0.1 480,550 + PGA1_c04890 0.20 -0.2 480,558 - PGA1_c04890 0.21 -0.5 480,583 - PGA1_c04890 0.23 +0.6 480,593 + PGA1_c04890 0.24 +0.3 480,661 + PGA1_c04890 0.30 +0.3 480,666 + PGA1_c04890 0.30 +0.0 480,678 + PGA1_c04890 0.31 +0.3 480,681 - PGA1_c04890 0.31 +0.3 480,692 + PGA1_c04890 0.32 +0.7 480,696 + PGA1_c04890 0.33 +3.2 480,803 - PGA1_c04890 0.42 +0.2 480,803 - PGA1_c04890 0.42 -0.7 480,803 - PGA1_c04890 0.42 -0.5 480,803 - PGA1_c04890 0.42 -1.5 480,803 - PGA1_c04890 0.42 +0.7 480,803 - PGA1_c04890 0.42 +0.4 480,803 - PGA1_c04890 0.42 +0.6 480,803 - PGA1_c04890 0.42 +0.4 480,891 + PGA1_c04890 0.49 -1.6 480,898 + PGA1_c04890 0.50 +0.8 480,899 - PGA1_c04890 0.50 -1.3 480,906 - PGA1_c04890 0.51 +1.1 480,934 + PGA1_c04890 0.53 +0.8 480,970 + PGA1_c04890 0.56 +0.4 480,978 - PGA1_c04890 0.57 -0.9 480,978 - PGA1_c04890 0.57 +0.8 481,007 + PGA1_c04890 0.59 +0.7 481,079 + PGA1_c04890 0.66 -0.0 481,142 + PGA1_c04890 0.71 -0.0 481,199 - PGA1_c04890 0.76 +0.8 481,249 + PGA1_c04890 0.80 -0.3 481,249 + PGA1_c04890 0.80 +1.3 481,259 + PGA1_c04890 0.81 -0.3 481,274 - PGA1_c04890 0.82 -1.3 481,274 - PGA1_c04890 0.82 -0.3 481,284 - PGA1_c04890 0.83 +0.5 481,284 - PGA1_c04890 0.83 -0.0
Or see this region's nucleotide sequence