Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04120 and PGA1_c04130 are separated by 89 nucleotides PGA1_c04130 and PGA1_c04140 overlap by 4 nucleotides
PGA1_c04120: PGA1_c04120 - 3-ketoacyl-CoA thiolase FadA, at 399,154 to 400,329
_c04120
PGA1_c04130: PGA1_c04130 - putative acyl-CoA dehydrogenase, at 400,419 to 401,618
_c04130
PGA1_c04140: PGA1_c04140 - putative acyl-CoA dehydrogenase, at 401,615 to 402,760
_c04140
Position (kb) 400
401
402 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 399.440 kb on + strand, within PGA1_c04120 at 399.448 kb on - strand, within PGA1_c04120 at 399.513 kb on - strand, within PGA1_c04120 at 399.520 kb on + strand, within PGA1_c04120 at 399.577 kb on - strand, within PGA1_c04120 at 399.577 kb on - strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.650 kb on + strand, within PGA1_c04120 at 399.656 kb on - strand, within PGA1_c04120 at 399.658 kb on - strand, within PGA1_c04120 at 399.658 kb on - strand, within PGA1_c04120 at 399.658 kb on - strand, within PGA1_c04120 at 399.658 kb on - strand, within PGA1_c04120 at 399.658 kb on - strand, within PGA1_c04120 at 399.658 kb on - strand, within PGA1_c04120 at 399.766 kb on - strand, within PGA1_c04120 at 399.828 kb on - strand, within PGA1_c04120 at 399.839 kb on + strand, within PGA1_c04120 at 399.894 kb on + strand, within PGA1_c04120 at 399.895 kb on + strand, within PGA1_c04120 at 399.902 kb on - strand, within PGA1_c04120 at 399.916 kb on + strand, within PGA1_c04120 at 399.934 kb on + strand, within PGA1_c04120 at 399.953 kb on + strand, within PGA1_c04120 at 399.961 kb on - strand, within PGA1_c04120 at 399.961 kb on - strand, within PGA1_c04120 at 399.961 kb on - strand, within PGA1_c04120 at 399.961 kb on - strand, within PGA1_c04120 at 400.051 kb on + strand, within PGA1_c04120 at 400.061 kb on + strand, within PGA1_c04120 at 400.093 kb on - strand, within PGA1_c04120 at 400.103 kb on - strand, within PGA1_c04120 at 400.103 kb on - strand, within PGA1_c04120 at 400.104 kb on - strand, within PGA1_c04120 at 400.155 kb on + strand, within PGA1_c04120 at 400.158 kb on - strand, within PGA1_c04120 at 400.222 kb on + strand at 400.230 kb on - strand at 400.233 kb on + strand at 400.281 kb on + strand at 400.307 kb on - strand at 400.336 kb on - strand at 400.354 kb on + strand at 400.362 kb on - strand at 400.362 kb on - strand at 400.362 kb on - strand at 400.400 kb on + strand at 400.491 kb on + strand at 400.499 kb on - strand at 400.522 kb on + strand at 400.522 kb on + strand at 400.527 kb on + strand at 400.540 kb on - strand, within PGA1_c04130 at 400.540 kb on - strand, within PGA1_c04130 at 400.585 kb on - strand, within PGA1_c04130 at 400.597 kb on + strand, within PGA1_c04130 at 400.600 kb on - strand, within PGA1_c04130 at 400.600 kb on - strand, within PGA1_c04130 at 400.629 kb on - strand, within PGA1_c04130 at 400.632 kb on + strand, within PGA1_c04130 at 400.677 kb on - strand, within PGA1_c04130 at 400.690 kb on + strand, within PGA1_c04130 at 400.698 kb on - strand, within PGA1_c04130 at 400.698 kb on - strand, within PGA1_c04130 at 400.743 kb on - strand, within PGA1_c04130 at 400.743 kb on - strand, within PGA1_c04130 at 400.743 kb on - strand, within PGA1_c04130 at 400.799 kb on - strand, within PGA1_c04130 at 400.800 kb on - strand, within PGA1_c04130 at 400.824 kb on + strand, within PGA1_c04130 at 400.832 kb on - strand, within PGA1_c04130 at 400.841 kb on + strand, within PGA1_c04130 at 400.851 kb on + strand, within PGA1_c04130 at 400.859 kb on - strand, within PGA1_c04130 at 400.900 kb on + strand, within PGA1_c04130 at 400.900 kb on + strand, within PGA1_c04130 at 400.908 kb on - strand, within PGA1_c04130 at 400.908 kb on - strand, within PGA1_c04130 at 400.908 kb on - strand, within PGA1_c04130 at 400.934 kb on + strand, within PGA1_c04130 at 400.934 kb on + strand, within PGA1_c04130 at 400.940 kb on + strand, within PGA1_c04130 at 401.002 kb on - strand, within PGA1_c04130 at 401.002 kb on - strand, within PGA1_c04130 at 401.005 kb on + strand, within PGA1_c04130 at 401.005 kb on + strand, within PGA1_c04130 at 401.013 kb on - strand, within PGA1_c04130 at 401.013 kb on - strand, within PGA1_c04130 at 401.115 kb on + strand, within PGA1_c04130 at 401.115 kb on + strand, within PGA1_c04130 at 401.115 kb on + strand, within PGA1_c04130 at 401.115 kb on + strand, within PGA1_c04130 at 401.116 kb on - strand, within PGA1_c04130 at 401.116 kb on - strand, within PGA1_c04130 at 401.116 kb on - strand, within PGA1_c04130 at 401.123 kb on - strand, within PGA1_c04130 at 401.159 kb on - strand, within PGA1_c04130 at 401.174 kb on - strand, within PGA1_c04130 at 401.222 kb on + strand, within PGA1_c04130 at 401.230 kb on - strand, within PGA1_c04130 at 401.316 kb on - strand, within PGA1_c04130 at 401.332 kb on - strand, within PGA1_c04130 at 401.348 kb on + strand, within PGA1_c04130 at 401.546 kb on + strand at 401.638 kb on + strand at 401.683 kb on + strand at 401.714 kb on - strand at 401.721 kb on + strand at 401.721 kb on + strand at 401.721 kb on + strand at 401.721 kb on + strand at 401.726 kb on + strand at 401.726 kb on + strand at 401.726 kb on + strand at 401.734 kb on - strand, within PGA1_c04140 at 401.816 kb on + strand, within PGA1_c04140 at 401.816 kb on + strand, within PGA1_c04140 at 401.824 kb on - strand, within PGA1_c04140 at 401.843 kb on + strand, within PGA1_c04140 at 401.843 kb on + strand, within PGA1_c04140 at 401.843 kb on + strand, within PGA1_c04140 at 401.843 kb on + strand, within PGA1_c04140 at 401.843 kb on + strand, within PGA1_c04140 at 401.851 kb on - strand, within PGA1_c04140 at 401.851 kb on - strand, within PGA1_c04140 at 401.851 kb on - strand, within PGA1_c04140 at 401.858 kb on + strand, within PGA1_c04140 at 401.885 kb on + strand, within PGA1_c04140 at 401.898 kb on + strand, within PGA1_c04140 at 402.025 kb on + strand, within PGA1_c04140 at 402.035 kb on - strand, within PGA1_c04140 at 402.165 kb on + strand, within PGA1_c04140 at 402.205 kb on - strand, within PGA1_c04140 at 402.248 kb on - strand, within PGA1_c04140 at 402.372 kb on - strand, within PGA1_c04140 at 402.374 kb on - strand, within PGA1_c04140 at 402.403 kb on + strand, within PGA1_c04140 at 402.497 kb on - strand, within PGA1_c04140 at 402.497 kb on - strand, within PGA1_c04140 at 402.499 kb on + strand, within PGA1_c04140 at 402.499 kb on + strand, within PGA1_c04140 at 402.512 kb on - strand, within PGA1_c04140 at 402.514 kb on + strand, within PGA1_c04140 at 402.514 kb on + strand, within PGA1_c04140
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 399,440 + PGA1_c04120 0.24 -1.3 399,448 - PGA1_c04120 0.25 +0.8 399,513 - PGA1_c04120 0.31 +0.1 399,520 + PGA1_c04120 0.31 -0.8 399,577 - PGA1_c04120 0.36 +0.1 399,577 - PGA1_c04120 0.36 +0.3 399,650 + PGA1_c04120 0.42 -0.9 399,650 + PGA1_c04120 0.42 -3.0 399,650 + PGA1_c04120 0.42 +0.6 399,650 + PGA1_c04120 0.42 +0.5 399,650 + PGA1_c04120 0.42 +1.2 399,650 + PGA1_c04120 0.42 -1.5 399,650 + PGA1_c04120 0.42 +0.9 399,650 + PGA1_c04120 0.42 +0.0 399,656 - PGA1_c04120 0.43 -0.0 399,658 - PGA1_c04120 0.43 +0.2 399,658 - PGA1_c04120 0.43 -1.1 399,658 - PGA1_c04120 0.43 -1.2 399,658 - PGA1_c04120 0.43 -0.1 399,658 - PGA1_c04120 0.43 +0.1 399,658 - PGA1_c04120 0.43 +0.0 399,766 - PGA1_c04120 0.52 -0.5 399,828 - PGA1_c04120 0.57 -0.4 399,839 + PGA1_c04120 0.58 -0.3 399,894 + PGA1_c04120 0.63 -2.0 399,895 + PGA1_c04120 0.63 -0.1 399,902 - PGA1_c04120 0.64 -0.1 399,916 + PGA1_c04120 0.65 +1.5 399,934 + PGA1_c04120 0.66 +0.7 399,953 + PGA1_c04120 0.68 -0.3 399,961 - PGA1_c04120 0.69 -2.2 399,961 - PGA1_c04120 0.69 -0.6 399,961 - PGA1_c04120 0.69 -1.8 399,961 - PGA1_c04120 0.69 +0.2 400,051 + PGA1_c04120 0.76 -0.0 400,061 + PGA1_c04120 0.77 +0.2 400,093 - PGA1_c04120 0.80 -0.6 400,103 - PGA1_c04120 0.81 +1.0 400,103 - PGA1_c04120 0.81 -1.7 400,104 - PGA1_c04120 0.81 +0.4 400,155 + PGA1_c04120 0.85 +0.8 400,158 - PGA1_c04120 0.85 +0.3 400,222 + +0.2 400,230 - +0.5 400,233 + -0.9 400,281 + -1.8 400,307 - -1.5 400,336 - -0.2 400,354 + -0.0 400,362 - +1.1 400,362 - +0.3 400,362 - -0.1 400,400 + +0.2 400,491 + -0.5 400,499 - -0.0 400,522 + -1.1 400,522 + -0.6 400,527 + -0.6 400,540 - PGA1_c04130 0.10 +0.4 400,540 - PGA1_c04130 0.10 +0.6 400,585 - PGA1_c04130 0.14 +0.1 400,597 + PGA1_c04130 0.15 +0.1 400,600 - PGA1_c04130 0.15 +0.5 400,600 - PGA1_c04130 0.15 +0.4 400,629 - PGA1_c04130 0.17 +0.1 400,632 + PGA1_c04130 0.18 -0.5 400,677 - PGA1_c04130 0.21 -0.8 400,690 + PGA1_c04130 0.23 -0.9 400,698 - PGA1_c04130 0.23 +0.1 400,698 - PGA1_c04130 0.23 +0.5 400,743 - PGA1_c04130 0.27 +0.4 400,743 - PGA1_c04130 0.27 -0.2 400,743 - PGA1_c04130 0.27 -0.6 400,799 - PGA1_c04130 0.32 +2.0 400,800 - PGA1_c04130 0.32 -0.8 400,824 + PGA1_c04130 0.34 -1.9 400,832 - PGA1_c04130 0.34 -1.0 400,841 + PGA1_c04130 0.35 -0.4 400,851 + PGA1_c04130 0.36 +0.9 400,859 - PGA1_c04130 0.37 -1.6 400,900 + PGA1_c04130 0.40 -0.1 400,900 + PGA1_c04130 0.40 -0.9 400,908 - PGA1_c04130 0.41 +0.2 400,908 - PGA1_c04130 0.41 -2.2 400,908 - PGA1_c04130 0.41 +0.9 400,934 + PGA1_c04130 0.43 +2.0 400,934 + PGA1_c04130 0.43 -0.3 400,940 + PGA1_c04130 0.43 +0.3 401,002 - PGA1_c04130 0.49 -0.4 401,002 - PGA1_c04130 0.49 -0.6 401,005 + PGA1_c04130 0.49 -1.5 401,005 + PGA1_c04130 0.49 +0.6 401,013 - PGA1_c04130 0.49 +0.3 401,013 - PGA1_c04130 0.49 -0.9 401,115 + PGA1_c04130 0.58 +1.2 401,115 + PGA1_c04130 0.58 -0.0 401,115 + PGA1_c04130 0.58 +0.6 401,115 + PGA1_c04130 0.58 +0.2 401,116 - PGA1_c04130 0.58 -0.6 401,116 - PGA1_c04130 0.58 +1.7 401,116 - PGA1_c04130 0.58 -0.1 401,123 - PGA1_c04130 0.59 -1.5 401,159 - PGA1_c04130 0.62 +0.9 401,174 - PGA1_c04130 0.63 -0.3 401,222 + PGA1_c04130 0.67 -0.8 401,230 - PGA1_c04130 0.68 -1.3 401,316 - PGA1_c04130 0.75 +0.8 401,332 - PGA1_c04130 0.76 +0.7 401,348 + PGA1_c04130 0.77 +0.9 401,546 + +1.7 401,638 + +0.0 401,683 + +1.7 401,714 - -1.5 401,721 + -0.4 401,721 + -0.1 401,721 + -1.2 401,721 + -0.2 401,726 + +0.5 401,726 + -1.4 401,726 + -0.1 401,734 - PGA1_c04140 0.10 -0.5 401,816 + PGA1_c04140 0.18 -1.3 401,816 + PGA1_c04140 0.18 -0.5 401,824 - PGA1_c04140 0.18 -1.6 401,843 + PGA1_c04140 0.20 +2.2 401,843 + PGA1_c04140 0.20 -1.3 401,843 + PGA1_c04140 0.20 -1.8 401,843 + PGA1_c04140 0.20 -0.2 401,843 + PGA1_c04140 0.20 -0.5 401,851 - PGA1_c04140 0.21 -0.3 401,851 - PGA1_c04140 0.21 +0.1 401,851 - PGA1_c04140 0.21 -0.2 401,858 + PGA1_c04140 0.21 +0.7 401,885 + PGA1_c04140 0.24 +0.0 401,898 + PGA1_c04140 0.25 +0.0 402,025 + PGA1_c04140 0.36 +0.5 402,035 - PGA1_c04140 0.37 -1.5 402,165 + PGA1_c04140 0.48 -1.2 402,205 - PGA1_c04140 0.51 -1.2 402,248 - PGA1_c04140 0.55 -1.1 402,372 - PGA1_c04140 0.66 -0.0 402,374 - PGA1_c04140 0.66 -1.6 402,403 + PGA1_c04140 0.69 -0.6 402,497 - PGA1_c04140 0.77 -2.7 402,497 - PGA1_c04140 0.77 -0.8 402,499 + PGA1_c04140 0.77 -0.9 402,499 + PGA1_c04140 0.77 +0.0 402,512 - PGA1_c04140 0.78 +1.3 402,514 + PGA1_c04140 0.78 +0.7 402,514 + PGA1_c04140 0.78 +1.5
Or see this region's nucleotide sequence