Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03710 and PGA1_c03720 are separated by 23 nucleotides PGA1_c03720 and PGA1_c03730 are separated by 32 nucleotides PGA1_c03730 and PGA1_c03740 are separated by 192 nucleotides
PGA1_c03710: PGA1_c03710 - Acyl dehydratase, at 356,777 to 357,808
_c03710
PGA1_c03720: PGA1_c03720 - phosphoglycerate mutase-like protein, at 357,832 to 358,461
_c03720
PGA1_c03730: PGA1_c03730 - Protein of unknown function (DUF1194)., at 358,494 to 359,183
_c03730
PGA1_c03740: PGA1_c03740 - succinate dehydrogenase cytochrome b556 subunit, at 359,376 to 359,759
_c03740
Position (kb)
357
358
359 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 356.869 kb on - strand at 356.937 kb on + strand, within PGA1_c03710 at 356.937 kb on + strand, within PGA1_c03710 at 356.945 kb on - strand, within PGA1_c03710 at 357.002 kb on + strand, within PGA1_c03710 at 357.072 kb on + strand, within PGA1_c03710 at 357.077 kb on + strand, within PGA1_c03710 at 357.078 kb on + strand, within PGA1_c03710 at 357.078 kb on + strand, within PGA1_c03710 at 357.078 kb on + strand, within PGA1_c03710 at 357.086 kb on - strand, within PGA1_c03710 at 357.086 kb on - strand, within PGA1_c03710 at 357.086 kb on - strand, within PGA1_c03710 at 357.086 kb on - strand, within PGA1_c03710 at 357.086 kb on - strand, within PGA1_c03710 at 357.146 kb on + strand, within PGA1_c03710 at 357.165 kb on + strand, within PGA1_c03710 at 357.167 kb on - strand, within PGA1_c03710 at 357.167 kb on - strand, within PGA1_c03710 at 357.167 kb on - strand, within PGA1_c03710 at 357.172 kb on - strand, within PGA1_c03710 at 357.172 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.173 kb on - strand, within PGA1_c03710 at 357.188 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.207 kb on + strand, within PGA1_c03710 at 357.215 kb on - strand, within PGA1_c03710 at 357.215 kb on - strand, within PGA1_c03710 at 357.215 kb on - strand, within PGA1_c03710 at 357.268 kb on + strand, within PGA1_c03710 at 357.268 kb on + strand, within PGA1_c03710 at 357.275 kb on - strand, within PGA1_c03710 at 357.281 kb on - strand, within PGA1_c03710 at 357.308 kb on + strand, within PGA1_c03710 at 357.348 kb on - strand, within PGA1_c03710 at 357.374 kb on - strand, within PGA1_c03710 at 357.394 kb on + strand, within PGA1_c03710 at 357.402 kb on - strand, within PGA1_c03710 at 357.408 kb on + strand, within PGA1_c03710 at 357.412 kb on + strand, within PGA1_c03710 at 357.435 kb on + strand, within PGA1_c03710 at 357.443 kb on - strand, within PGA1_c03710 at 357.495 kb on - strand, within PGA1_c03710 at 357.500 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.505 kb on + strand, within PGA1_c03710 at 357.513 kb on - strand, within PGA1_c03710 at 357.513 kb on - strand, within PGA1_c03710 at 357.521 kb on - strand, within PGA1_c03710 at 357.533 kb on - strand, within PGA1_c03710 at 357.589 kb on - strand, within PGA1_c03710 at 357.600 kb on + strand, within PGA1_c03710 at 357.607 kb on + strand, within PGA1_c03710 at 357.615 kb on - strand, within PGA1_c03710 at 357.620 kb on + strand, within PGA1_c03710 at 357.647 kb on + strand, within PGA1_c03710 at 357.647 kb on - strand, within PGA1_c03710 at 357.652 kb on + strand, within PGA1_c03710 at 357.655 kb on - strand, within PGA1_c03710 at 357.659 kb on + strand, within PGA1_c03710 at 357.659 kb on + strand, within PGA1_c03710 at 357.659 kb on + strand, within PGA1_c03710 at 357.667 kb on - strand, within PGA1_c03710 at 357.667 kb on - strand, within PGA1_c03710 at 357.667 kb on - strand, within PGA1_c03710 at 357.722 kb on - strand at 357.722 kb on - strand at 357.851 kb on + strand at 357.871 kb on + strand at 357.916 kb on - strand, within PGA1_c03720 at 357.967 kb on + strand, within PGA1_c03720 at 357.972 kb on + strand, within PGA1_c03720 at 357.972 kb on + strand, within PGA1_c03720 at 357.972 kb on + strand, within PGA1_c03720 at 357.980 kb on - strand, within PGA1_c03720 at 358.004 kb on - strand, within PGA1_c03720 at 358.009 kb on - strand, within PGA1_c03720 at 358.011 kb on + strand, within PGA1_c03720 at 358.019 kb on - strand, within PGA1_c03720 at 358.036 kb on + strand, within PGA1_c03720 at 358.083 kb on - strand, within PGA1_c03720 at 358.103 kb on + strand, within PGA1_c03720 at 358.108 kb on + strand, within PGA1_c03720 at 358.112 kb on - strand, within PGA1_c03720 at 358.135 kb on - strand, within PGA1_c03720 at 358.160 kb on + strand, within PGA1_c03720 at 358.174 kb on + strand, within PGA1_c03720 at 358.174 kb on + strand, within PGA1_c03720 at 358.174 kb on + strand, within PGA1_c03720 at 358.174 kb on + strand, within PGA1_c03720 at 358.174 kb on + strand, within PGA1_c03720 at 358.181 kb on + strand, within PGA1_c03720 at 358.182 kb on - strand, within PGA1_c03720 at 358.182 kb on - strand, within PGA1_c03720 at 358.182 kb on - strand, within PGA1_c03720 at 358.182 kb on - strand, within PGA1_c03720 at 358.182 kb on - strand, within PGA1_c03720 at 358.206 kb on + strand, within PGA1_c03720 at 358.214 kb on - strand, within PGA1_c03720 at 358.227 kb on + strand, within PGA1_c03720 at 358.267 kb on + strand, within PGA1_c03720 at 358.271 kb on - strand, within PGA1_c03720 at 358.291 kb on + strand, within PGA1_c03720 at 358.296 kb on + strand, within PGA1_c03720 at 358.296 kb on + strand, within PGA1_c03720 at 358.296 kb on + strand, within PGA1_c03720 at 358.296 kb on + strand, within PGA1_c03720 at 358.301 kb on + strand, within PGA1_c03720 at 358.301 kb on + strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.304 kb on - strand, within PGA1_c03720 at 358.307 kb on - strand, within PGA1_c03720 at 358.309 kb on - strand, within PGA1_c03720 at 358.334 kb on + strand, within PGA1_c03720 at 358.345 kb on - strand, within PGA1_c03720 at 358.355 kb on - strand, within PGA1_c03720 at 358.377 kb on - strand, within PGA1_c03720 at 358.387 kb on - strand, within PGA1_c03720 at 358.388 kb on + strand, within PGA1_c03720 at 358.388 kb on + strand, within PGA1_c03720 at 358.396 kb on + strand, within PGA1_c03720 at 358.398 kb on + strand, within PGA1_c03720 at 358.406 kb on - strand at 358.406 kb on - strand at 358.440 kb on - strand at 358.456 kb on + strand at 358.456 kb on + strand at 358.456 kb on + strand at 358.461 kb on + strand at 358.464 kb on - strand at 358.464 kb on - strand at 358.530 kb on + strand at 358.546 kb on - strand at 358.555 kb on + strand at 358.555 kb on + strand at 358.555 kb on + strand at 358.555 kb on + strand at 358.563 kb on - strand, within PGA1_c03730 at 358.568 kb on - strand, within PGA1_c03730 at 358.586 kb on + strand, within PGA1_c03730 at 358.589 kb on - strand, within PGA1_c03730 at 358.613 kb on + strand, within PGA1_c03730 at 358.623 kb on - strand, within PGA1_c03730 at 358.625 kb on + strand, within PGA1_c03730 at 358.625 kb on + strand, within PGA1_c03730 at 358.632 kb on + strand, within PGA1_c03730 at 358.633 kb on - strand, within PGA1_c03730 at 358.633 kb on - strand, within PGA1_c03730 at 358.633 kb on - strand, within PGA1_c03730 at 358.652 kb on + strand, within PGA1_c03730 at 358.665 kb on + strand, within PGA1_c03730 at 358.671 kb on + strand, within PGA1_c03730 at 358.671 kb on + strand, within PGA1_c03730 at 358.679 kb on - strand, within PGA1_c03730 at 358.679 kb on - strand, within PGA1_c03730 at 358.679 kb on - strand, within PGA1_c03730 at 358.763 kb on + strand, within PGA1_c03730 at 358.770 kb on + strand, within PGA1_c03730 at 358.778 kb on - strand, within PGA1_c03730 at 358.825 kb on - strand, within PGA1_c03730 at 358.834 kb on + strand, within PGA1_c03730 at 358.840 kb on - strand, within PGA1_c03730 at 358.842 kb on - strand, within PGA1_c03730 at 358.853 kb on + strand, within PGA1_c03730 at 358.866 kb on - strand, within PGA1_c03730 at 358.876 kb on - strand, within PGA1_c03730 at 358.881 kb on + strand, within PGA1_c03730 at 358.897 kb on - strand, within PGA1_c03730 at 358.943 kb on - strand, within PGA1_c03730 at 358.944 kb on - strand, within PGA1_c03730 at 358.944 kb on - strand, within PGA1_c03730 at 358.999 kb on + strand, within PGA1_c03730 at 358.999 kb on + strand, within PGA1_c03730 at 358.999 kb on + strand, within PGA1_c03730 at 359.017 kb on - strand, within PGA1_c03730 at 359.053 kb on - strand, within PGA1_c03730 at 359.065 kb on + strand, within PGA1_c03730 at 359.065 kb on + strand, within PGA1_c03730 at 359.070 kb on - strand, within PGA1_c03730 at 359.073 kb on - strand, within PGA1_c03730 at 359.086 kb on + strand, within PGA1_c03730 at 359.094 kb on - strand, within PGA1_c03730 at 359.094 kb on - strand, within PGA1_c03730 at 359.106 kb on - strand, within PGA1_c03730 at 359.139 kb on - strand at 359.159 kb on - strand at 359.161 kb on + strand at 359.161 kb on + strand at 359.161 kb on + strand at 359.169 kb on - strand at 359.169 kb on - strand at 359.169 kb on - strand at 359.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 356,869 - +1.1 356,937 + PGA1_c03710 0.16 -0.6 356,937 + PGA1_c03710 0.16 +0.7 356,945 - PGA1_c03710 0.16 -0.3 357,002 + PGA1_c03710 0.22 +0.1 357,072 + PGA1_c03710 0.29 -1.5 357,077 + PGA1_c03710 0.29 +1.0 357,078 + PGA1_c03710 0.29 +0.4 357,078 + PGA1_c03710 0.29 -0.4 357,078 + PGA1_c03710 0.29 -0.6 357,086 - PGA1_c03710 0.30 -1.3 357,086 - PGA1_c03710 0.30 -0.3 357,086 - PGA1_c03710 0.30 +1.5 357,086 - PGA1_c03710 0.30 +0.7 357,086 - PGA1_c03710 0.30 -1.0 357,146 + PGA1_c03710 0.36 -1.8 357,165 + PGA1_c03710 0.38 +0.3 357,167 - PGA1_c03710 0.38 +0.2 357,167 - PGA1_c03710 0.38 -0.7 357,167 - PGA1_c03710 0.38 -0.3 357,172 - PGA1_c03710 0.38 +0.7 357,172 - PGA1_c03710 0.38 +0.7 357,173 - PGA1_c03710 0.38 +0.6 357,173 - PGA1_c03710 0.38 -0.1 357,173 - PGA1_c03710 0.38 +0.7 357,173 - PGA1_c03710 0.38 +0.9 357,173 - PGA1_c03710 0.38 +0.1 357,173 - PGA1_c03710 0.38 -0.9 357,173 - PGA1_c03710 0.38 -2.3 357,173 - PGA1_c03710 0.38 +0.9 357,173 - PGA1_c03710 0.38 -1.1 357,173 - PGA1_c03710 0.38 +0.4 357,188 + PGA1_c03710 0.40 -3.1 357,207 + PGA1_c03710 0.42 -1.7 357,207 + PGA1_c03710 0.42 -0.5 357,207 + PGA1_c03710 0.42 -1.5 357,207 + PGA1_c03710 0.42 -1.2 357,207 + PGA1_c03710 0.42 -0.1 357,207 + PGA1_c03710 0.42 -2.0 357,207 + PGA1_c03710 0.42 -0.2 357,215 - PGA1_c03710 0.42 -0.8 357,215 - PGA1_c03710 0.42 -0.1 357,215 - PGA1_c03710 0.42 +1.0 357,268 + PGA1_c03710 0.48 -1.0 357,268 + PGA1_c03710 0.48 +0.8 357,275 - PGA1_c03710 0.48 -0.6 357,281 - PGA1_c03710 0.49 +0.3 357,308 + PGA1_c03710 0.51 +0.3 357,348 - PGA1_c03710 0.55 +0.6 357,374 - PGA1_c03710 0.58 +0.8 357,394 + PGA1_c03710 0.60 -1.3 357,402 - PGA1_c03710 0.61 +0.7 357,408 + PGA1_c03710 0.61 +0.3 357,412 + PGA1_c03710 0.62 +1.4 357,435 + PGA1_c03710 0.64 -3.0 357,443 - PGA1_c03710 0.65 -1.4 357,495 - PGA1_c03710 0.70 -2.7 357,500 + PGA1_c03710 0.70 +0.1 357,505 + PGA1_c03710 0.71 +2.1 357,505 + PGA1_c03710 0.71 -1.5 357,505 + PGA1_c03710 0.71 -0.3 357,505 + PGA1_c03710 0.71 -1.9 357,505 + PGA1_c03710 0.71 +1.9 357,505 + PGA1_c03710 0.71 -1.8 357,505 + PGA1_c03710 0.71 +0.5 357,505 + PGA1_c03710 0.71 -0.5 357,513 - PGA1_c03710 0.71 +1.3 357,513 - PGA1_c03710 0.71 -0.3 357,521 - PGA1_c03710 0.72 -1.2 357,533 - PGA1_c03710 0.73 -1.5 357,589 - PGA1_c03710 0.79 +1.4 357,600 + PGA1_c03710 0.80 +0.4 357,607 + PGA1_c03710 0.80 +0.1 357,615 - PGA1_c03710 0.81 +0.8 357,620 + PGA1_c03710 0.82 +0.3 357,647 + PGA1_c03710 0.84 -0.0 357,647 - PGA1_c03710 0.84 +2.0 357,652 + PGA1_c03710 0.85 -0.3 357,655 - PGA1_c03710 0.85 +0.7 357,659 + PGA1_c03710 0.85 -1.2 357,659 + PGA1_c03710 0.85 -0.8 357,659 + PGA1_c03710 0.85 +0.1 357,667 - PGA1_c03710 0.86 +1.4 357,667 - PGA1_c03710 0.86 -0.5 357,667 - PGA1_c03710 0.86 +0.4 357,722 - -0.1 357,722 - +1.7 357,851 + +0.0 357,871 + +0.2 357,916 - PGA1_c03720 0.13 -0.3 357,967 + PGA1_c03720 0.21 -0.6 357,972 + PGA1_c03720 0.22 -0.4 357,972 + PGA1_c03720 0.22 +0.6 357,972 + PGA1_c03720 0.22 +0.8 357,980 - PGA1_c03720 0.23 -0.3 358,004 - PGA1_c03720 0.27 +1.6 358,009 - PGA1_c03720 0.28 +0.8 358,011 + PGA1_c03720 0.28 +0.3 358,019 - PGA1_c03720 0.30 +1.7 358,036 + PGA1_c03720 0.32 -1.6 358,083 - PGA1_c03720 0.40 -1.1 358,103 + PGA1_c03720 0.43 -1.2 358,108 + PGA1_c03720 0.44 -0.2 358,112 - PGA1_c03720 0.44 -0.5 358,135 - PGA1_c03720 0.48 -1.2 358,160 + PGA1_c03720 0.52 -1.7 358,174 + PGA1_c03720 0.54 +0.4 358,174 + PGA1_c03720 0.54 -0.5 358,174 + PGA1_c03720 0.54 -1.2 358,174 + PGA1_c03720 0.54 +0.1 358,174 + PGA1_c03720 0.54 -1.1 358,181 + PGA1_c03720 0.55 -1.9 358,182 - PGA1_c03720 0.56 -2.8 358,182 - PGA1_c03720 0.56 +1.7 358,182 - PGA1_c03720 0.56 +0.7 358,182 - PGA1_c03720 0.56 +0.5 358,182 - PGA1_c03720 0.56 +1.9 358,206 + PGA1_c03720 0.59 -0.4 358,214 - PGA1_c03720 0.61 -1.5 358,227 + PGA1_c03720 0.63 -0.6 358,267 + PGA1_c03720 0.69 -1.0 358,271 - PGA1_c03720 0.70 +0.1 358,291 + PGA1_c03720 0.73 -0.9 358,296 + PGA1_c03720 0.74 +0.9 358,296 + PGA1_c03720 0.74 +0.5 358,296 + PGA1_c03720 0.74 -0.9 358,296 + PGA1_c03720 0.74 +0.9 358,301 + PGA1_c03720 0.74 -1.4 358,301 + PGA1_c03720 0.74 -0.4 358,304 - PGA1_c03720 0.75 +0.4 358,304 - PGA1_c03720 0.75 +0.7 358,304 - PGA1_c03720 0.75 -0.1 358,304 - PGA1_c03720 0.75 +0.3 358,304 - PGA1_c03720 0.75 +1.3 358,304 - PGA1_c03720 0.75 +0.1 358,304 - PGA1_c03720 0.75 -0.1 358,304 - PGA1_c03720 0.75 -0.8 358,304 - PGA1_c03720 0.75 -0.3 358,304 - PGA1_c03720 0.75 +1.1 358,304 - PGA1_c03720 0.75 -0.1 358,304 - PGA1_c03720 0.75 +0.4 358,304 - PGA1_c03720 0.75 +0.3 358,304 - PGA1_c03720 0.75 -0.6 358,307 - PGA1_c03720 0.75 -0.5 358,309 - PGA1_c03720 0.76 -0.1 358,334 + PGA1_c03720 0.80 -0.7 358,345 - PGA1_c03720 0.81 -0.5 358,355 - PGA1_c03720 0.83 +0.7 358,377 - PGA1_c03720 0.87 +1.2 358,387 - PGA1_c03720 0.88 -0.1 358,388 + PGA1_c03720 0.88 -0.4 358,388 + PGA1_c03720 0.88 -0.9 358,396 + PGA1_c03720 0.90 -0.1 358,398 + PGA1_c03720 0.90 +0.2 358,406 - -0.3 358,406 - +0.7 358,440 - +3.3 358,456 + +0.7 358,456 + -0.0 358,456 + -2.5 358,461 + +0.3 358,464 - -0.5 358,464 - +0.6 358,530 + +0.7 358,546 - +0.7 358,555 + -1.7 358,555 + +2.0 358,555 + +0.4 358,555 + -1.3 358,563 - PGA1_c03730 0.10 -0.7 358,568 - PGA1_c03730 0.11 -0.6 358,586 + PGA1_c03730 0.13 -1.9 358,589 - PGA1_c03730 0.14 +1.1 358,613 + PGA1_c03730 0.17 +1.2 358,623 - PGA1_c03730 0.19 +0.7 358,625 + PGA1_c03730 0.19 +0.9 358,625 + PGA1_c03730 0.19 -0.9 358,632 + PGA1_c03730 0.20 -0.3 358,633 - PGA1_c03730 0.20 -0.7 358,633 - PGA1_c03730 0.20 -0.2 358,633 - PGA1_c03730 0.20 -0.8 358,652 + PGA1_c03730 0.23 +0.0 358,665 + PGA1_c03730 0.25 +1.1 358,671 + PGA1_c03730 0.26 -0.2 358,671 + PGA1_c03730 0.26 -0.6 358,679 - PGA1_c03730 0.27 -1.6 358,679 - PGA1_c03730 0.27 +1.0 358,679 - PGA1_c03730 0.27 +1.1 358,763 + PGA1_c03730 0.39 -1.6 358,770 + PGA1_c03730 0.40 +0.2 358,778 - PGA1_c03730 0.41 -0.3 358,825 - PGA1_c03730 0.48 +1.4 358,834 + PGA1_c03730 0.49 +0.8 358,840 - PGA1_c03730 0.50 +0.5 358,842 - PGA1_c03730 0.50 -0.4 358,853 + PGA1_c03730 0.52 -0.1 358,866 - PGA1_c03730 0.54 +0.8 358,876 - PGA1_c03730 0.55 -2.4 358,881 + PGA1_c03730 0.56 -0.6 358,897 - PGA1_c03730 0.58 +0.7 358,943 - PGA1_c03730 0.65 -2.7 358,944 - PGA1_c03730 0.65 +0.4 358,944 - PGA1_c03730 0.65 +0.4 358,999 + PGA1_c03730 0.73 +1.7 358,999 + PGA1_c03730 0.73 -2.1 358,999 + PGA1_c03730 0.73 +0.0 359,017 - PGA1_c03730 0.76 +0.7 359,053 - PGA1_c03730 0.81 +0.3 359,065 + PGA1_c03730 0.83 +0.1 359,065 + PGA1_c03730 0.83 -0.1 359,070 - PGA1_c03730 0.83 -1.5 359,073 - PGA1_c03730 0.84 -0.1 359,086 + PGA1_c03730 0.86 +0.9 359,094 - PGA1_c03730 0.87 +0.3 359,094 - PGA1_c03730 0.87 +2.0 359,106 - PGA1_c03730 0.89 -0.9 359,139 - -1.1 359,159 - -0.5 359,161 + -1.6 359,161 + -0.5 359,161 + +1.0 359,169 - +0.1 359,169 - -0.2 359,169 - -0.4 359,203 - -1.1
Or see this region's nucleotide sequence