Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00070 and PGA1_262p00080 are separated by 28 nucleotides PGA1_262p00080 and PGA1_262p00090 are separated by 7 nucleotides PGA1_262p00090 and PGA1_262p00100 are separated by 142 nucleotides
PGA1_262p00070: PGA1_262p00070 - Lipid A core - O-antigen ligase and related enzymes, at 7,972 to 9,258
_262p00070
PGA1_262p00080: PGA1_262p00080 - hypothetical protein, at 9,287 to 10,054
_262p00080
PGA1_262p00090: PGA1_262p00090 - putative exopolysaccharide biosynthesis transport protein, at 10,062 to 12,173
_262p00090
PGA1_262p00100: PGA1_262p00100 - putative glycosyl transferase, at 12,316 to 13,404
_262p00100
Position (kb) 10
11
12
13 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 9.079 kb on - strand, within PGA1_262p00070 at 9.103 kb on - strand, within PGA1_262p00070 at 9.252 kb on + strand at 9.376 kb on - strand, within PGA1_262p00080 at 9.472 kb on - strand, within PGA1_262p00080 at 9.540 kb on - strand, within PGA1_262p00080 at 9.777 kb on - strand, within PGA1_262p00080 at 9.779 kb on + strand, within PGA1_262p00080 at 9.779 kb on + strand, within PGA1_262p00080 at 9.855 kb on - strand, within PGA1_262p00080 at 9.965 kb on - strand, within PGA1_262p00080 at 9.983 kb on + strand at 10.051 kb on + strand at 10.065 kb on + strand at 10.123 kb on + strand at 10.131 kb on - strand at 10.213 kb on + strand at 10.221 kb on - strand at 10.545 kb on - strand, within PGA1_262p00090 at 10.734 kb on + strand, within PGA1_262p00090 at 10.857 kb on - strand, within PGA1_262p00090 at 11.039 kb on - strand, within PGA1_262p00090 at 11.162 kb on + strand, within PGA1_262p00090 at 11.170 kb on - strand, within PGA1_262p00090 at 11.262 kb on - strand, within PGA1_262p00090 at 11.275 kb on + strand, within PGA1_262p00090 at 11.352 kb on - strand, within PGA1_262p00090 at 11.580 kb on - strand, within PGA1_262p00090 at 11.610 kb on + strand, within PGA1_262p00090 at 11.646 kb on + strand, within PGA1_262p00090 at 11.654 kb on - strand, within PGA1_262p00090 at 11.843 kb on + strand, within PGA1_262p00090 at 11.875 kb on - strand, within PGA1_262p00090 at 11.877 kb on + strand, within PGA1_262p00090 at 12.103 kb on - strand at 12.157 kb on - strand at 12.179 kb on + strand at 12.187 kb on - strand at 12.296 kb on - strand at 12.554 kb on + strand, within PGA1_262p00100 at 12.644 kb on + strand, within PGA1_262p00100 at 12.644 kb on + strand, within PGA1_262p00100 at 12.853 kb on + strand, within PGA1_262p00100 at 12.860 kb on - strand, within PGA1_262p00100 at 12.942 kb on + strand, within PGA1_262p00100 at 13.045 kb on + strand, within PGA1_262p00100 at 13.052 kb on + strand, within PGA1_262p00100
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 9,079 - PGA1_262p00070 0.86 -0.8 9,103 - PGA1_262p00070 0.88 -2.0 9,252 + -1.5 9,376 - PGA1_262p00080 0.12 -0.1 9,472 - PGA1_262p00080 0.24 -0.6 9,540 - PGA1_262p00080 0.33 -1.1 9,777 - PGA1_262p00080 0.64 +0.6 9,779 + PGA1_262p00080 0.64 +2.2 9,779 + PGA1_262p00080 0.64 +2.0 9,855 - PGA1_262p00080 0.74 +0.2 9,965 - PGA1_262p00080 0.88 +0.0 9,983 + +0.2 10,051 + +1.5 10,065 + -0.8 10,123 + -0.4 10,131 - +0.2 10,213 + -1.1 10,221 - -0.7 10,545 - PGA1_262p00090 0.23 +0.8 10,734 + PGA1_262p00090 0.32 +0.4 10,857 - PGA1_262p00090 0.38 -0.1 11,039 - PGA1_262p00090 0.46 +0.4 11,162 + PGA1_262p00090 0.52 -1.1 11,170 - PGA1_262p00090 0.52 +1.6 11,262 - PGA1_262p00090 0.57 -0.0 11,275 + PGA1_262p00090 0.57 -0.1 11,352 - PGA1_262p00090 0.61 +2.6 11,580 - PGA1_262p00090 0.72 +0.2 11,610 + PGA1_262p00090 0.73 +0.6 11,646 + PGA1_262p00090 0.75 +0.8 11,654 - PGA1_262p00090 0.75 +0.9 11,843 + PGA1_262p00090 0.84 +2.2 11,875 - PGA1_262p00090 0.86 +2.6 11,877 + PGA1_262p00090 0.86 +0.9 12,103 - -0.8 12,157 - +1.9 12,179 + +0.2 12,187 - -2.5 12,296 - +0.6 12,554 + PGA1_262p00100 0.22 +1.2 12,644 + PGA1_262p00100 0.30 -0.3 12,644 + PGA1_262p00100 0.30 -0.6 12,853 + PGA1_262p00100 0.49 -1.4 12,860 - PGA1_262p00100 0.50 -3.1 12,942 + PGA1_262p00100 0.57 +2.5 13,045 + PGA1_262p00100 0.67 +0.6 13,052 + PGA1_262p00100 0.68 -0.3
Or see this region's nucleotide sequence