Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35660 and PGA1_c35670 overlap by 1 nucleotides PGA1_c35670 and PGA1_c35680 are separated by 77 nucleotides PGA1_c35680 and PGA1_c35690 overlap by 1 nucleotides
PGA1_c35660: PGA1_c35660 - hypothetical protein, at 3,738,529 to 3,738,993
_c35660
PGA1_c35670: PGA1_c35670 - Uncharacterized conserved protein, at 3,738,993 to 3,739,640
_c35670
PGA1_c35680: PGA1_c35680 - chemotaxis protein MotA, at 3,739,718 to 3,740,587
_c35680
PGA1_c35690: PGA1_c35690 - hypothetical protein, at 3,740,587 to 3,743,043
_c35690
Position (kb)
3739
3740
3741 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3738.730 kb on + strand, within PGA1_c35660 at 3738.730 kb on + strand, within PGA1_c35660 at 3738.733 kb on - strand, within PGA1_c35660 at 3738.773 kb on + strand, within PGA1_c35660 at 3738.906 kb on + strand, within PGA1_c35660 at 3738.924 kb on + strand, within PGA1_c35660 at 3738.954 kb on - strand at 3738.978 kb on + strand at 3738.978 kb on + strand at 3738.978 kb on + strand at 3738.978 kb on + strand at 3738.978 kb on + strand at 3738.978 kb on + strand at 3738.978 kb on + strand at 3738.995 kb on + strand at 3739.076 kb on + strand, within PGA1_c35670 at 3739.094 kb on + strand, within PGA1_c35670 at 3739.150 kb on - strand, within PGA1_c35670 at 3739.258 kb on + strand, within PGA1_c35670 at 3739.258 kb on + strand, within PGA1_c35670 at 3739.347 kb on + strand, within PGA1_c35670 at 3739.348 kb on + strand, within PGA1_c35670 at 3739.349 kb on + strand, within PGA1_c35670 at 3739.371 kb on - strand, within PGA1_c35670 at 3739.373 kb on + strand, within PGA1_c35670 at 3739.419 kb on + strand, within PGA1_c35670 at 3739.428 kb on + strand, within PGA1_c35670 at 3739.428 kb on + strand, within PGA1_c35670 at 3739.428 kb on + strand, within PGA1_c35670 at 3739.436 kb on - strand, within PGA1_c35670 at 3739.460 kb on + strand, within PGA1_c35670 at 3739.468 kb on - strand, within PGA1_c35670 at 3739.500 kb on + strand, within PGA1_c35670 at 3739.500 kb on + strand, within PGA1_c35670 at 3739.508 kb on - strand, within PGA1_c35670 at 3739.563 kb on + strand, within PGA1_c35670 at 3739.602 kb on + strand at 3739.609 kb on - strand at 3739.616 kb on + strand at 3739.621 kb on + strand at 3739.626 kb on + strand at 3739.626 kb on + strand at 3739.626 kb on + strand at 3739.629 kb on - strand at 3739.634 kb on + strand at 3739.634 kb on + strand at 3739.634 kb on + strand at 3739.637 kb on - strand at 3739.642 kb on - strand at 3739.712 kb on + strand at 3739.720 kb on - strand at 3739.725 kb on + strand at 3739.739 kb on + strand at 3739.759 kb on + strand at 3739.774 kb on - strand at 3739.781 kb on + strand at 3739.781 kb on + strand at 3739.801 kb on + strand at 3739.801 kb on + strand at 3739.801 kb on + strand at 3739.801 kb on + strand at 3739.839 kb on + strand, within PGA1_c35680 at 3739.847 kb on + strand, within PGA1_c35680 at 3739.855 kb on - strand, within PGA1_c35680 at 3739.855 kb on - strand, within PGA1_c35680 at 3739.881 kb on + strand, within PGA1_c35680 at 3739.881 kb on + strand, within PGA1_c35680 at 3739.881 kb on + strand, within PGA1_c35680 at 3739.889 kb on - strand, within PGA1_c35680 at 3739.889 kb on - strand, within PGA1_c35680 at 3739.915 kb on + strand, within PGA1_c35680 at 3739.921 kb on - strand, within PGA1_c35680 at 3739.921 kb on - strand, within PGA1_c35680 at 3739.923 kb on - strand, within PGA1_c35680 at 3739.963 kb on - strand, within PGA1_c35680 at 3739.978 kb on + strand, within PGA1_c35680 at 3740.093 kb on - strand, within PGA1_c35680 at 3740.154 kb on - strand, within PGA1_c35680 at 3740.154 kb on - strand, within PGA1_c35680 at 3740.154 kb on - strand, within PGA1_c35680 at 3740.198 kb on + strand, within PGA1_c35680 at 3740.208 kb on - strand, within PGA1_c35680 at 3740.232 kb on + strand, within PGA1_c35680 at 3740.245 kb on + strand, within PGA1_c35680 at 3740.317 kb on - strand, within PGA1_c35680 at 3740.331 kb on - strand, within PGA1_c35680 at 3740.385 kb on - strand, within PGA1_c35680 at 3740.398 kb on + strand, within PGA1_c35680 at 3740.411 kb on + strand, within PGA1_c35680 at 3740.411 kb on + strand, within PGA1_c35680 at 3740.498 kb on - strand, within PGA1_c35680 at 3740.514 kb on + strand at 3740.514 kb on + strand at 3740.514 kb on + strand at 3740.522 kb on - strand at 3740.582 kb on + strand at 3740.590 kb on - strand at 3740.603 kb on + strand at 3740.604 kb on - strand at 3740.619 kb on - strand at 3740.624 kb on - strand at 3740.624 kb on - strand at 3740.648 kb on - strand at 3740.650 kb on + strand at 3740.650 kb on + strand at 3740.708 kb on - strand at 3740.715 kb on + strand at 3740.766 kb on + strand at 3740.828 kb on - strand at 3740.838 kb on + strand, within PGA1_c35690 at 3740.838 kb on + strand, within PGA1_c35690 at 3740.838 kb on + strand, within PGA1_c35690 at 3740.838 kb on + strand, within PGA1_c35690 at 3740.846 kb on - strand, within PGA1_c35690 at 3740.862 kb on - strand, within PGA1_c35690 at 3740.902 kb on - strand, within PGA1_c35690 at 3740.907 kb on - strand, within PGA1_c35690 at 3740.948 kb on + strand, within PGA1_c35690 at 3740.948 kb on + strand, within PGA1_c35690 at 3740.958 kb on + strand, within PGA1_c35690 at 3740.958 kb on + strand, within PGA1_c35690 at 3740.958 kb on + strand, within PGA1_c35690 at 3740.958 kb on + strand, within PGA1_c35690 at 3740.961 kb on - strand, within PGA1_c35690 at 3741.044 kb on + strand, within PGA1_c35690 at 3741.100 kb on + strand, within PGA1_c35690 at 3741.138 kb on + strand, within PGA1_c35690 at 3741.169 kb on + strand, within PGA1_c35690 at 3741.177 kb on - strand, within PGA1_c35690 at 3741.234 kb on + strand, within PGA1_c35690 at 3741.263 kb on + strand, within PGA1_c35690 at 3741.278 kb on + strand, within PGA1_c35690 at 3741.278 kb on + strand, within PGA1_c35690 at 3741.278 kb on + strand, within PGA1_c35690 at 3741.398 kb on - strand, within PGA1_c35690 at 3741.422 kb on - strand, within PGA1_c35690 at 3741.429 kb on - strand, within PGA1_c35690 at 3741.444 kb on - strand, within PGA1_c35690 at 3741.444 kb on - strand, within PGA1_c35690 at 3741.496 kb on - strand, within PGA1_c35690 at 3741.496 kb on - strand, within PGA1_c35690 at 3741.499 kb on - strand, within PGA1_c35690 at 3741.527 kb on + strand, within PGA1_c35690 at 3741.527 kb on + strand, within PGA1_c35690 at 3741.535 kb on - strand, within PGA1_c35690 at 3741.535 kb on - strand, within PGA1_c35690 at 3741.536 kb on + strand, within PGA1_c35690 at 3741.536 kb on + strand, within PGA1_c35690 at 3741.544 kb on - strand, within PGA1_c35690 at 3741.544 kb on - strand, within PGA1_c35690 at 3741.544 kb on - strand, within PGA1_c35690 at 3741.544 kb on - strand, within PGA1_c35690 at 3741.544 kb on - strand, within PGA1_c35690 at 3741.544 kb on - strand, within PGA1_c35690 at 3741.566 kb on + strand, within PGA1_c35690
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 3,738,730 + PGA1_c35660 0.43 -1.4 3,738,730 + PGA1_c35660 0.43 -2.6 3,738,733 - PGA1_c35660 0.44 -1.8 3,738,773 + PGA1_c35660 0.52 +0.2 3,738,906 + PGA1_c35660 0.81 -0.2 3,738,924 + PGA1_c35660 0.85 -2.4 3,738,954 - -1.3 3,738,978 + -2.4 3,738,978 + +0.0 3,738,978 + -1.6 3,738,978 + +0.4 3,738,978 + -0.3 3,738,978 + -1.2 3,738,978 + -2.3 3,738,995 + -1.1 3,739,076 + PGA1_c35670 0.13 -1.9 3,739,094 + PGA1_c35670 0.16 -0.4 3,739,150 - PGA1_c35670 0.24 -1.9 3,739,258 + PGA1_c35670 0.41 -2.2 3,739,258 + PGA1_c35670 0.41 -1.9 3,739,347 + PGA1_c35670 0.55 -2.3 3,739,348 + PGA1_c35670 0.55 -0.9 3,739,349 + PGA1_c35670 0.55 -0.5 3,739,371 - PGA1_c35670 0.58 -0.4 3,739,373 + PGA1_c35670 0.59 -1.3 3,739,419 + PGA1_c35670 0.66 -1.1 3,739,428 + PGA1_c35670 0.67 -0.2 3,739,428 + PGA1_c35670 0.67 -0.9 3,739,428 + PGA1_c35670 0.67 -1.2 3,739,436 - PGA1_c35670 0.68 -2.3 3,739,460 + PGA1_c35670 0.72 -1.2 3,739,468 - PGA1_c35670 0.73 -1.2 3,739,500 + PGA1_c35670 0.78 -1.2 3,739,500 + PGA1_c35670 0.78 -0.5 3,739,508 - PGA1_c35670 0.79 -1.2 3,739,563 + PGA1_c35670 0.88 -2.4 3,739,602 + -1.9 3,739,609 - -1.8 3,739,616 + -0.3 3,739,621 + +0.2 3,739,626 + -1.0 3,739,626 + -0.6 3,739,626 + -1.8 3,739,629 - -1.6 3,739,634 + -1.6 3,739,634 + +1.0 3,739,634 + +1.2 3,739,637 - -1.9 3,739,642 - +1.2 3,739,712 + -0.7 3,739,720 - -3.0 3,739,725 + -1.2 3,739,739 + -1.1 3,739,759 + -1.1 3,739,774 - -0.4 3,739,781 + +0.2 3,739,781 + -2.0 3,739,801 + -0.8 3,739,801 + +2.1 3,739,801 + -0.8 3,739,801 + -1.6 3,739,839 + PGA1_c35680 0.14 -1.6 3,739,847 + PGA1_c35680 0.15 -2.6 3,739,855 - PGA1_c35680 0.16 -2.7 3,739,855 - PGA1_c35680 0.16 -2.4 3,739,881 + PGA1_c35680 0.19 -0.6 3,739,881 + PGA1_c35680 0.19 -1.4 3,739,881 + PGA1_c35680 0.19 -2.2 3,739,889 - PGA1_c35680 0.20 -0.5 3,739,889 - PGA1_c35680 0.20 -1.3 3,739,915 + PGA1_c35680 0.23 +0.2 3,739,921 - PGA1_c35680 0.23 -1.6 3,739,921 - PGA1_c35680 0.23 -0.2 3,739,923 - PGA1_c35680 0.24 -2.5 3,739,963 - PGA1_c35680 0.28 +0.9 3,739,978 + PGA1_c35680 0.30 -1.6 3,740,093 - PGA1_c35680 0.43 +0.2 3,740,154 - PGA1_c35680 0.50 -2.6 3,740,154 - PGA1_c35680 0.50 -1.2 3,740,154 - PGA1_c35680 0.50 -2.5 3,740,198 + PGA1_c35680 0.55 -2.4 3,740,208 - PGA1_c35680 0.56 -2.4 3,740,232 + PGA1_c35680 0.59 -0.5 3,740,245 + PGA1_c35680 0.61 -1.3 3,740,317 - PGA1_c35680 0.69 -2.3 3,740,331 - PGA1_c35680 0.70 -2.6 3,740,385 - PGA1_c35680 0.77 -0.9 3,740,398 + PGA1_c35680 0.78 +0.6 3,740,411 + PGA1_c35680 0.80 -0.4 3,740,411 + PGA1_c35680 0.80 +0.2 3,740,498 - PGA1_c35680 0.90 -0.9 3,740,514 + -1.5 3,740,514 + -1.2 3,740,514 + -1.6 3,740,522 - -0.9 3,740,582 + -1.5 3,740,590 - +0.1 3,740,603 + -1.2 3,740,604 - -1.5 3,740,619 - -2.4 3,740,624 - +0.0 3,740,624 - -2.4 3,740,648 - -2.1 3,740,650 + -0.1 3,740,650 + -0.1 3,740,708 - +0.0 3,740,715 + -0.5 3,740,766 + +0.7 3,740,828 - -1.1 3,740,838 + PGA1_c35690 0.10 -1.2 3,740,838 + PGA1_c35690 0.10 -1.0 3,740,838 + PGA1_c35690 0.10 -1.1 3,740,838 + PGA1_c35690 0.10 +1.3 3,740,846 - PGA1_c35690 0.11 -0.8 3,740,862 - PGA1_c35690 0.11 -2.4 3,740,902 - PGA1_c35690 0.13 -2.5 3,740,907 - PGA1_c35690 0.13 -1.9 3,740,948 + PGA1_c35690 0.15 -0.2 3,740,948 + PGA1_c35690 0.15 -1.4 3,740,958 + PGA1_c35690 0.15 -1.4 3,740,958 + PGA1_c35690 0.15 -3.0 3,740,958 + PGA1_c35690 0.15 -1.1 3,740,958 + PGA1_c35690 0.15 -0.8 3,740,961 - PGA1_c35690 0.15 -1.2 3,741,044 + PGA1_c35690 0.19 -1.6 3,741,100 + PGA1_c35690 0.21 -2.1 3,741,138 + PGA1_c35690 0.22 -1.5 3,741,169 + PGA1_c35690 0.24 -1.8 3,741,177 - PGA1_c35690 0.24 -1.9 3,741,234 + PGA1_c35690 0.26 -0.6 3,741,263 + PGA1_c35690 0.28 -1.0 3,741,278 + PGA1_c35690 0.28 -1.3 3,741,278 + PGA1_c35690 0.28 -2.2 3,741,278 + PGA1_c35690 0.28 -1.7 3,741,398 - PGA1_c35690 0.33 -2.0 3,741,422 - PGA1_c35690 0.34 -0.3 3,741,429 - PGA1_c35690 0.34 -0.9 3,741,444 - PGA1_c35690 0.35 -1.2 3,741,444 - PGA1_c35690 0.35 -0.7 3,741,496 - PGA1_c35690 0.37 -1.5 3,741,496 - PGA1_c35690 0.37 -1.4 3,741,499 - PGA1_c35690 0.37 -0.8 3,741,527 + PGA1_c35690 0.38 -1.7 3,741,527 + PGA1_c35690 0.38 -2.1 3,741,535 - PGA1_c35690 0.39 -0.2 3,741,535 - PGA1_c35690 0.39 -2.8 3,741,536 + PGA1_c35690 0.39 -1.8 3,741,536 + PGA1_c35690 0.39 -3.6 3,741,544 - PGA1_c35690 0.39 +0.2 3,741,544 - PGA1_c35690 0.39 -2.3 3,741,544 - PGA1_c35690 0.39 -2.0 3,741,544 - PGA1_c35690 0.39 -0.1 3,741,544 - PGA1_c35690 0.39 -0.3 3,741,544 - PGA1_c35690 0.39 -1.4 3,741,566 + PGA1_c35690 0.40 -3.3
Or see this region's nucleotide sequence