Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34250 and PGA1_c34260 are separated by 141 nucleotides PGA1_c34260 and PGA1_c34270 are separated by 208 nucleotides PGA1_c34270 and PGA1_c34280 are separated by 112 nucleotides PGA1_c34280 and PGA1_c34290 overlap by 4 nucleotides
PGA1_c34250: PGA1_c34250 - AMP nucleosidase Amn, at 3,590,153 to 3,591,634
_c34250
PGA1_c34260: PGA1_c34260 - DNA-binding protein HU-beta, at 3,591,776 to 3,592,063
_c34260
PGA1_c34270: PGA1_c34270 - putative integral membrane protein, at 3,592,272 to 3,593,156
_c34270
PGA1_c34280: PGA1_c34280 - peptide methionine sulfoxide reductase MsrB, at 3,593,269 to 3,593,718
_c34280
PGA1_c34290: PGA1_c34290 - peptide methionine sulfoxide reductase MsrA, at 3,593,715 to 3,594,236
_c34290
Position (kb)
3592
3593
3594 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3591.326 kb on - strand, within PGA1_c34250 at 3591.344 kb on - strand, within PGA1_c34250 at 3591.377 kb on + strand, within PGA1_c34250 at 3591.391 kb on - strand, within PGA1_c34250 at 3591.391 kb on - strand, within PGA1_c34250 at 3591.543 kb on + strand at 3591.543 kb on + strand at 3591.543 kb on + strand at 3591.594 kb on + strand at 3591.594 kb on + strand at 3591.594 kb on + strand at 3591.594 kb on + strand at 3591.594 kb on + strand at 3591.594 kb on + strand at 3591.594 kb on + strand at 3591.609 kb on + strand at 3591.757 kb on + strand at 3591.757 kb on + strand at 3591.757 kb on + strand at 3591.765 kb on - strand at 3591.765 kb on - strand at 3591.804 kb on - strand at 3592.064 kb on + strand at 3592.072 kb on - strand at 3592.072 kb on - strand at 3592.075 kb on + strand at 3592.084 kb on - strand at 3592.142 kb on - strand at 3592.144 kb on + strand at 3592.152 kb on - strand at 3592.304 kb on - strand at 3592.308 kb on + strand at 3592.308 kb on + strand at 3592.308 kb on + strand at 3592.316 kb on - strand at 3592.316 kb on - strand at 3592.322 kb on - strand at 3592.334 kb on - strand at 3592.334 kb on - strand at 3592.351 kb on - strand at 3592.429 kb on + strand, within PGA1_c34270 at 3592.433 kb on - strand, within PGA1_c34270 at 3592.434 kb on + strand, within PGA1_c34270 at 3592.434 kb on + strand, within PGA1_c34270 at 3592.437 kb on - strand, within PGA1_c34270 at 3592.437 kb on - strand, within PGA1_c34270 at 3592.442 kb on - strand, within PGA1_c34270 at 3592.447 kb on - strand, within PGA1_c34270 at 3592.577 kb on + strand, within PGA1_c34270 at 3592.584 kb on - strand, within PGA1_c34270 at 3592.607 kb on + strand, within PGA1_c34270 at 3592.755 kb on - strand, within PGA1_c34270 at 3592.762 kb on + strand, within PGA1_c34270 at 3592.762 kb on + strand, within PGA1_c34270 at 3592.762 kb on + strand, within PGA1_c34270 at 3592.762 kb on + strand, within PGA1_c34270 at 3592.762 kb on + strand, within PGA1_c34270 at 3592.770 kb on - strand, within PGA1_c34270 at 3592.770 kb on - strand, within PGA1_c34270 at 3592.820 kb on - strand, within PGA1_c34270 at 3592.820 kb on - strand, within PGA1_c34270 at 3592.821 kb on + strand, within PGA1_c34270 at 3592.839 kb on - strand, within PGA1_c34270 at 3592.935 kb on - strand, within PGA1_c34270 at 3593.033 kb on + strand, within PGA1_c34270 at 3593.041 kb on - strand, within PGA1_c34270 at 3593.128 kb on + strand at 3593.138 kb on + strand at 3593.141 kb on - strand at 3593.196 kb on - strand at 3593.217 kb on - strand at 3593.228 kb on - strand at 3593.248 kb on - strand at 3593.263 kb on - strand at 3593.279 kb on + strand at 3593.287 kb on - strand at 3593.307 kb on - strand at 3593.307 kb on - strand at 3593.340 kb on - strand, within PGA1_c34280 at 3593.425 kb on + strand, within PGA1_c34280 at 3593.478 kb on - strand, within PGA1_c34280 at 3593.479 kb on - strand, within PGA1_c34280 at 3593.524 kb on - strand, within PGA1_c34280 at 3593.545 kb on - strand, within PGA1_c34280 at 3593.552 kb on - strand, within PGA1_c34280 at 3593.569 kb on + strand, within PGA1_c34280 at 3593.602 kb on - strand, within PGA1_c34280 at 3593.624 kb on - strand, within PGA1_c34280 at 3593.706 kb on + strand at 3593.725 kb on + strand at 3593.736 kb on + strand at 3593.969 kb on + strand, within PGA1_c34290 at 3594.044 kb on - strand, within PGA1_c34290 at 3594.070 kb on - strand, within PGA1_c34290
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 3,591,326 - PGA1_c34250 0.79 +1.4 3,591,344 - PGA1_c34250 0.80 -0.3 3,591,377 + PGA1_c34250 0.83 -1.8 3,591,391 - PGA1_c34250 0.84 -0.5 3,591,391 - PGA1_c34250 0.84 -0.9 3,591,543 + -0.5 3,591,543 + -0.4 3,591,543 + -2.6 3,591,594 + +1.5 3,591,594 + -0.5 3,591,594 + +0.3 3,591,594 + +0.7 3,591,594 + -0.4 3,591,594 + -2.7 3,591,594 + -1.4 3,591,609 + +0.2 3,591,757 + -1.2 3,591,757 + -0.4 3,591,757 + -1.2 3,591,765 - +1.2 3,591,765 - +0.8 3,591,804 - -0.2 3,592,064 + +0.2 3,592,072 - -0.4 3,592,072 - -1.3 3,592,075 + -0.5 3,592,084 - -0.8 3,592,142 - -2.2 3,592,144 + +0.0 3,592,152 - -0.2 3,592,304 - -0.4 3,592,308 + +1.0 3,592,308 + +0.1 3,592,308 + -0.2 3,592,316 - -1.0 3,592,316 - +0.5 3,592,322 - +0.3 3,592,334 - +0.7 3,592,334 - +0.4 3,592,351 - +0.3 3,592,429 + PGA1_c34270 0.18 +1.5 3,592,433 - PGA1_c34270 0.18 +1.0 3,592,434 + PGA1_c34270 0.18 -1.9 3,592,434 + PGA1_c34270 0.18 -0.7 3,592,437 - PGA1_c34270 0.19 +0.2 3,592,437 - PGA1_c34270 0.19 -2.6 3,592,442 - PGA1_c34270 0.19 +1.8 3,592,447 - PGA1_c34270 0.20 -1.2 3,592,577 + PGA1_c34270 0.34 +1.4 3,592,584 - PGA1_c34270 0.35 +0.1 3,592,607 + PGA1_c34270 0.38 +0.2 3,592,755 - PGA1_c34270 0.55 -0.5 3,592,762 + PGA1_c34270 0.55 +1.3 3,592,762 + PGA1_c34270 0.55 +0.2 3,592,762 + PGA1_c34270 0.55 -0.2 3,592,762 + PGA1_c34270 0.55 -0.3 3,592,762 + PGA1_c34270 0.55 +0.4 3,592,770 - PGA1_c34270 0.56 +0.2 3,592,770 - PGA1_c34270 0.56 +0.9 3,592,820 - PGA1_c34270 0.62 -1.6 3,592,820 - PGA1_c34270 0.62 +1.2 3,592,821 + PGA1_c34270 0.62 -0.4 3,592,839 - PGA1_c34270 0.64 -0.4 3,592,935 - PGA1_c34270 0.75 +1.4 3,593,033 + PGA1_c34270 0.86 -0.0 3,593,041 - PGA1_c34270 0.87 -0.8 3,593,128 + +0.7 3,593,138 + -0.5 3,593,141 - -2.4 3,593,196 - -1.4 3,593,217 - +0.2 3,593,228 - -0.6 3,593,248 - -2.3 3,593,263 - -1.2 3,593,279 + -0.7 3,593,287 - +0.3 3,593,307 - -1.0 3,593,307 - -1.4 3,593,340 - PGA1_c34280 0.16 -0.1 3,593,425 + PGA1_c34280 0.35 +0.3 3,593,478 - PGA1_c34280 0.46 +0.3 3,593,479 - PGA1_c34280 0.47 +1.2 3,593,524 - PGA1_c34280 0.57 -0.4 3,593,545 - PGA1_c34280 0.61 +0.5 3,593,552 - PGA1_c34280 0.63 -0.6 3,593,569 + PGA1_c34280 0.67 -0.8 3,593,602 - PGA1_c34280 0.74 +0.3 3,593,624 - PGA1_c34280 0.79 +0.0 3,593,706 + +0.5 3,593,725 + +0.3 3,593,736 + +0.9 3,593,969 + PGA1_c34290 0.49 +2.0 3,594,044 - PGA1_c34290 0.63 -0.3 3,594,070 - PGA1_c34290 0.68 -0.1
Or see this region's nucleotide sequence