Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03420 and PGA1_c03430 overlap by 8 nucleotides PGA1_c03430 and PGA1_c03440 are separated by 119 nucleotides PGA1_c03440 and PGA1_c03450 are separated by 22 nucleotides
PGA1_c03420: PGA1_c03420 - putative C-5 sterol desaturase, at 328,918 to 329,847
_c03420
PGA1_c03430: PGA1_c03430 - hypothetical protein, at 329,840 to 330,280
_c03430
PGA1_c03440: PGA1_c03440 - disulfide interchange protein tlpA-like protein, at 330,400 to 330,987
_c03440
PGA1_c03450: PGA1_c03450 - argininosuccinate lyase ArgH, at 331,010 to 332,380
_c03450
Position (kb)
329
330
331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.849 kb on - strand at 328.849 kb on - strand at 328.915 kb on + strand at 328.916 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.921 kb on + strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.942 kb on - strand at 328.944 kb on + strand at 328.949 kb on + strand at 328.952 kb on - strand at 328.980 kb on + strand at 328.980 kb on + strand at 328.988 kb on - strand at 329.011 kb on + strand, within PGA1_c03420 at 329.099 kb on - strand, within PGA1_c03420 at 329.115 kb on + strand, within PGA1_c03420 at 329.135 kb on - strand, within PGA1_c03420 at 329.139 kb on + strand, within PGA1_c03420 at 329.143 kb on - strand, within PGA1_c03420 at 329.154 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.203 kb on - strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.269 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.284 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.303 kb on - strand, within PGA1_c03420 at 329.305 kb on + strand, within PGA1_c03420 at 329.324 kb on + strand, within PGA1_c03420 at 329.344 kb on - strand, within PGA1_c03420 at 329.358 kb on - strand, within PGA1_c03420 at 329.388 kb on + strand, within PGA1_c03420 at 329.404 kb on + strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.441 kb on - strand, within PGA1_c03420 at 329.447 kb on + strand, within PGA1_c03420 at 329.514 kb on + strand, within PGA1_c03420 at 329.520 kb on - strand, within PGA1_c03420 at 329.522 kb on + strand, within PGA1_c03420 at 329.547 kb on + strand, within PGA1_c03420 at 329.552 kb on + strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.606 kb on + strand, within PGA1_c03420 at 329.629 kb on - strand, within PGA1_c03420 at 329.682 kb on + strand, within PGA1_c03420 at 329.714 kb on - strand, within PGA1_c03420 at 329.721 kb on - strand, within PGA1_c03420 at 329.833 kb on - strand at 329.857 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.882 kb on - strand at 329.884 kb on + strand, within PGA1_c03430 at 329.892 kb on - strand, within PGA1_c03430 at 329.921 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 330.007 kb on + strand, within PGA1_c03430 at 330.020 kb on - strand, within PGA1_c03430 at 330.022 kb on + strand, within PGA1_c03430 at 330.030 kb on - strand, within PGA1_c03430 at 330.057 kb on + strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.067 kb on + strand, within PGA1_c03430 at 330.082 kb on - strand, within PGA1_c03430 at 330.094 kb on + strand, within PGA1_c03430 at 330.097 kb on - strand, within PGA1_c03430 at 330.103 kb on + strand, within PGA1_c03430 at 330.155 kb on + strand, within PGA1_c03430 at 330.171 kb on - strand, within PGA1_c03430 at 330.172 kb on - strand, within PGA1_c03430 at 330.196 kb on + strand, within PGA1_c03430 at 330.198 kb on - strand, within PGA1_c03430 at 330.250 kb on + strand at 330.287 kb on + strand at 330.332 kb on - strand at 330.333 kb on - strand at 330.367 kb on + strand at 330.375 kb on - strand at 330.385 kb on + strand at 330.393 kb on - strand at 330.402 kb on + strand at 330.402 kb on + strand at 330.405 kb on - strand at 330.801 kb on + strand, within PGA1_c03440 at 330.969 kb on - strand at 330.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 328,841 + +1.9 328,841 + -0.6 328,841 + -0.6 328,841 + -0.1 328,849 - -2.8 328,849 - -1.2 328,915 + -1.4 328,916 - -0.3 328,918 - +0.7 328,918 - +0.9 328,918 - +1.7 328,918 - +0.7 328,921 + -0.6 328,923 - -1.1 328,923 - +0.1 328,923 - +0.3 328,923 - -2.8 328,923 - -0.5 328,942 - +0.3 328,944 + -0.3 328,949 + -2.0 328,952 - -1.3 328,980 + -1.0 328,980 + +0.3 328,988 - -0.8 329,011 + PGA1_c03420 0.10 +0.2 329,099 - PGA1_c03420 0.19 -0.4 329,115 + PGA1_c03420 0.21 -0.2 329,135 - PGA1_c03420 0.23 -0.1 329,139 + PGA1_c03420 0.24 -2.3 329,143 - PGA1_c03420 0.24 -0.8 329,154 - PGA1_c03420 0.25 -0.7 329,169 - PGA1_c03420 0.27 -1.2 329,169 - PGA1_c03420 0.27 +0.3 329,203 - PGA1_c03420 0.31 -0.6 329,217 + PGA1_c03420 0.32 +0.7 329,217 + PGA1_c03420 0.32 +0.2 329,269 + PGA1_c03420 0.38 -1.5 329,274 + PGA1_c03420 0.38 +0.7 329,274 + PGA1_c03420 0.38 +0.4 329,274 + PGA1_c03420 0.38 -0.6 329,284 + PGA1_c03420 0.39 +0.2 329,295 + PGA1_c03420 0.41 -0.3 329,295 + PGA1_c03420 0.41 -1.1 329,303 - PGA1_c03420 0.41 -1.3 329,305 + PGA1_c03420 0.42 -0.1 329,324 + PGA1_c03420 0.44 +0.4 329,344 - PGA1_c03420 0.46 +0.9 329,358 - PGA1_c03420 0.47 -1.5 329,388 + PGA1_c03420 0.51 -0.8 329,404 + PGA1_c03420 0.52 -1.3 329,412 - PGA1_c03420 0.53 -2.9 329,412 - PGA1_c03420 0.53 +1.3 329,441 - PGA1_c03420 0.56 +0.2 329,447 + PGA1_c03420 0.57 -2.3 329,514 + PGA1_c03420 0.64 -0.7 329,520 - PGA1_c03420 0.65 -0.3 329,522 + PGA1_c03420 0.65 -0.8 329,547 + PGA1_c03420 0.68 +1.1 329,552 + PGA1_c03420 0.68 -0.3 329,558 - PGA1_c03420 0.69 -0.1 329,558 - PGA1_c03420 0.69 +0.3 329,606 + PGA1_c03420 0.74 +0.1 329,629 - PGA1_c03420 0.76 +0.9 329,682 + PGA1_c03420 0.82 -0.4 329,714 - PGA1_c03420 0.86 -0.7 329,721 - PGA1_c03420 0.86 -2.1 329,833 - +0.9 329,857 - -2.8 329,871 - -1.3 329,871 - +0.4 329,871 - +1.0 329,882 - -0.1 329,884 + PGA1_c03430 0.10 -0.2 329,892 - PGA1_c03430 0.12 +0.6 329,921 + PGA1_c03430 0.18 -2.3 329,994 + PGA1_c03430 0.35 -0.1 329,994 + PGA1_c03430 0.35 -0.8 330,007 + PGA1_c03430 0.38 +0.4 330,020 - PGA1_c03430 0.41 +0.1 330,022 + PGA1_c03430 0.41 +1.7 330,030 - PGA1_c03430 0.43 +0.5 330,057 + PGA1_c03430 0.49 -0.3 330,065 - PGA1_c03430 0.51 -0.1 330,065 - PGA1_c03430 0.51 -0.1 330,067 + PGA1_c03430 0.51 -1.6 330,082 - PGA1_c03430 0.55 -1.4 330,094 + PGA1_c03430 0.58 -0.6 330,097 - PGA1_c03430 0.58 -0.7 330,103 + PGA1_c03430 0.60 +0.4 330,155 + PGA1_c03430 0.71 +1.1 330,171 - PGA1_c03430 0.75 +0.3 330,172 - PGA1_c03430 0.75 +0.6 330,196 + PGA1_c03430 0.81 -1.3 330,198 - PGA1_c03430 0.81 -0.4 330,250 + -1.4 330,287 + +0.5 330,332 - -1.4 330,333 - +1.7 330,367 + +0.5 330,375 - -0.6 330,385 + -0.4 330,393 - +0.3 330,402 + +0.2 330,402 + +1.2 330,405 - +0.2 330,801 + PGA1_c03440 0.68 +2.7 330,969 - +0.4 330,982 + -1.4
Or see this region's nucleotide sequence