Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c29100 and PGA1_c29110 are separated by 160 nucleotides PGA1_c29110 and PGA1_c29120 are separated by 70 nucleotides PGA1_c29120 and PGA1_c29130 are separated by 47 nucleotides PGA1_c29130 and PGA1_c29140 overlap by 4 nucleotides
PGA1_c29100: PGA1_c29100 - 2,4-dienoyl-CoA reductase FadH, at 3,048,745 to 3,050,772
_c29100
PGA1_c29110: PGA1_c29110 - intracellular protease PfpI, at 3,050,933 to 3,051,484
_c29110
PGA1_c29120: PGA1_c29120 - hypothetical protein, at 3,051,555 to 3,051,902
_c29120
PGA1_c29130: PGA1_c29130 - antibiotic biosynthesis monooxygenase-like protein, at 3,051,950 to 3,052,306
_c29130
PGA1_c29140: PGA1_c29140 - NIPSNAP., at 3,052,303 to 3,052,626
_c29140
Position (kb)
3050
3051
3052 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3049.934 kb on + strand, within PGA1_c29100 at 3049.934 kb on + strand, within PGA1_c29100 at 3049.934 kb on + strand, within PGA1_c29100 at 3049.942 kb on - strand, within PGA1_c29100 at 3049.948 kb on - strand, within PGA1_c29100 at 3049.980 kb on + strand, within PGA1_c29100 at 3049.991 kb on - strand, within PGA1_c29100 at 3050.037 kb on + strand, within PGA1_c29100 at 3050.104 kb on + strand, within PGA1_c29100 at 3050.264 kb on + strand, within PGA1_c29100 at 3050.264 kb on + strand, within PGA1_c29100 at 3050.289 kb on - strand, within PGA1_c29100 at 3050.304 kb on - strand, within PGA1_c29100 at 3050.379 kb on - strand, within PGA1_c29100 at 3050.438 kb on - strand, within PGA1_c29100 at 3050.457 kb on - strand, within PGA1_c29100 at 3050.563 kb on - strand, within PGA1_c29100 at 3050.605 kb on + strand at 3050.620 kb on - strand at 3050.620 kb on - strand at 3050.620 kb on - strand at 3050.620 kb on - strand at 3050.622 kb on - strand at 3050.632 kb on - strand at 3050.674 kb on + strand at 3050.676 kb on - strand at 3050.681 kb on - strand at 3050.974 kb on + strand at 3050.979 kb on + strand at 3050.979 kb on + strand at 3051.030 kb on + strand, within PGA1_c29110 at 3051.038 kb on - strand, within PGA1_c29110 at 3051.081 kb on - strand, within PGA1_c29110 at 3051.097 kb on - strand, within PGA1_c29110 at 3051.157 kb on + strand, within PGA1_c29110 at 3051.161 kb on - strand, within PGA1_c29110 at 3051.188 kb on - strand, within PGA1_c29110 at 3051.228 kb on - strand, within PGA1_c29110 at 3051.241 kb on + strand, within PGA1_c29110 at 3051.241 kb on + strand, within PGA1_c29110 at 3051.249 kb on - strand, within PGA1_c29110 at 3051.298 kb on + strand, within PGA1_c29110 at 3051.306 kb on - strand, within PGA1_c29110 at 3051.306 kb on - strand, within PGA1_c29110 at 3051.380 kb on - strand, within PGA1_c29110 at 3051.479 kb on + strand at 3051.493 kb on - strand at 3051.541 kb on - strand at 3051.561 kb on - strand at 3051.562 kb on - strand at 3051.636 kb on - strand, within PGA1_c29120 at 3051.636 kb on - strand, within PGA1_c29120 at 3051.660 kb on - strand, within PGA1_c29120 at 3051.916 kb on - strand at 3051.973 kb on - strand at 3051.994 kb on - strand, within PGA1_c29130 at 3052.106 kb on - strand, within PGA1_c29130 at 3052.176 kb on - strand, within PGA1_c29130 at 3052.187 kb on - strand, within PGA1_c29130 at 3052.198 kb on + strand, within PGA1_c29130 at 3052.215 kb on + strand, within PGA1_c29130 at 3052.313 kb on - strand at 3052.354 kb on - strand, within PGA1_c29140 at 3052.354 kb on - strand, within PGA1_c29140 at 3052.362 kb on + strand, within PGA1_c29140 at 3052.362 kb on + strand, within PGA1_c29140 at 3052.370 kb on - strand, within PGA1_c29140 at 3052.370 kb on - strand, within PGA1_c29140 at 3052.423 kb on - strand, within PGA1_c29140 at 3052.480 kb on - strand, within PGA1_c29140 at 3052.480 kb on - strand, within PGA1_c29140
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 3,049,934 + PGA1_c29100 0.59 -1.6 3,049,934 + PGA1_c29100 0.59 +0.1 3,049,934 + PGA1_c29100 0.59 -0.3 3,049,942 - PGA1_c29100 0.59 +1.3 3,049,948 - PGA1_c29100 0.59 -0.1 3,049,980 + PGA1_c29100 0.61 +0.4 3,049,991 - PGA1_c29100 0.61 -0.9 3,050,037 + PGA1_c29100 0.64 -1.6 3,050,104 + PGA1_c29100 0.67 +0.2 3,050,264 + PGA1_c29100 0.75 +0.1 3,050,264 + PGA1_c29100 0.75 -0.6 3,050,289 - PGA1_c29100 0.76 +1.3 3,050,304 - PGA1_c29100 0.77 +0.7 3,050,379 - PGA1_c29100 0.81 +0.1 3,050,438 - PGA1_c29100 0.83 -0.3 3,050,457 - PGA1_c29100 0.84 +0.7 3,050,563 - PGA1_c29100 0.90 +0.1 3,050,605 + -0.6 3,050,620 - +2.1 3,050,620 - -0.9 3,050,620 - +0.7 3,050,620 - -0.6 3,050,622 - +0.7 3,050,632 - +0.7 3,050,674 + -0.6 3,050,676 - -0.0 3,050,681 - -0.3 3,050,974 + +1.7 3,050,979 + +2.7 3,050,979 + -0.3 3,051,030 + PGA1_c29110 0.18 -0.9 3,051,038 - PGA1_c29110 0.19 -0.6 3,051,081 - PGA1_c29110 0.27 +0.7 3,051,097 - PGA1_c29110 0.30 +0.1 3,051,157 + PGA1_c29110 0.41 +1.2 3,051,161 - PGA1_c29110 0.41 +2.7 3,051,188 - PGA1_c29110 0.46 -1.6 3,051,228 - PGA1_c29110 0.53 -0.3 3,051,241 + PGA1_c29110 0.56 -0.9 3,051,241 + PGA1_c29110 0.56 -2.0 3,051,249 - PGA1_c29110 0.57 +0.1 3,051,298 + PGA1_c29110 0.66 +1.1 3,051,306 - PGA1_c29110 0.68 -0.3 3,051,306 - PGA1_c29110 0.68 -0.3 3,051,380 - PGA1_c29110 0.81 -1.5 3,051,479 + +0.1 3,051,493 - -0.8 3,051,541 - +1.7 3,051,561 - +0.7 3,051,562 - +0.7 3,051,636 - PGA1_c29120 0.23 +0.4 3,051,636 - PGA1_c29120 0.23 +1.7 3,051,660 - PGA1_c29120 0.30 -0.3 3,051,916 - +0.7 3,051,973 - +1.1 3,051,994 - PGA1_c29130 0.12 -1.9 3,052,106 - PGA1_c29130 0.44 +0.4 3,052,176 - PGA1_c29130 0.63 -1.1 3,052,187 - PGA1_c29130 0.66 +1.7 3,052,198 + PGA1_c29130 0.69 +0.1 3,052,215 + PGA1_c29130 0.74 +1.7 3,052,313 - +0.7 3,052,354 - PGA1_c29140 0.16 +0.7 3,052,354 - PGA1_c29140 0.16 +0.1 3,052,362 + PGA1_c29140 0.18 -0.1 3,052,362 + PGA1_c29140 0.18 +1.3 3,052,370 - PGA1_c29140 0.21 +0.1 3,052,370 - PGA1_c29140 0.21 -0.4 3,052,423 - PGA1_c29140 0.37 +0.7 3,052,480 - PGA1_c29140 0.55 +1.1 3,052,480 - PGA1_c29140 0.55 -0.7
Or see this region's nucleotide sequence