Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c27020 and PGA1_c27030 overlap by 8 nucleotides PGA1_c27030 and PGA1_c27040 are separated by 66 nucleotides PGA1_c27040 and PGA1_c27050 are separated by 41 nucleotides
PGA1_c27020: PGA1_c27020 - Protein of unknown function (DUF1469)., at 2,817,682 to 2,818,092
_c27020
PGA1_c27030: PGA1_c27030 - hypothetical protein, at 2,818,085 to 2,818,819
_c27030
PGA1_c27040: PGA1_c27040 - Predicted permease, at 2,818,886 to 2,819,908
_c27040
PGA1_c27050: PGA1_c27050 - phospholipase D-like protein, at 2,819,950 to 2,821,467
_c27050
Position (kb)
2818
2819
2820 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2817.919 kb on - strand, within PGA1_c27020 at 2818.005 kb on + strand, within PGA1_c27020 at 2818.064 kb on + strand at 2818.064 kb on + strand at 2818.072 kb on - strand at 2818.081 kb on + strand at 2818.089 kb on - strand at 2818.089 kb on - strand at 2818.103 kb on - strand at 2818.135 kb on - strand at 2818.137 kb on + strand at 2818.145 kb on - strand at 2818.145 kb on - strand at 2818.147 kb on + strand at 2818.155 kb on - strand at 2818.155 kb on - strand at 2818.212 kb on - strand, within PGA1_c27030 at 2818.217 kb on + strand, within PGA1_c27030 at 2818.260 kb on - strand, within PGA1_c27030 at 2818.331 kb on - strand, within PGA1_c27030 at 2818.331 kb on - strand, within PGA1_c27030 at 2818.336 kb on - strand, within PGA1_c27030 at 2818.336 kb on - strand, within PGA1_c27030 at 2818.532 kb on + strand, within PGA1_c27030 at 2818.540 kb on - strand, within PGA1_c27030 at 2818.558 kb on - strand, within PGA1_c27030 at 2818.558 kb on - strand, within PGA1_c27030 at 2818.600 kb on - strand, within PGA1_c27030 at 2818.608 kb on - strand, within PGA1_c27030 at 2818.625 kb on + strand, within PGA1_c27030 at 2818.635 kb on + strand, within PGA1_c27030 at 2818.777 kb on + strand at 2818.777 kb on + strand at 2818.785 kb on - strand at 2818.807 kb on - strand at 2818.818 kb on + strand at 2818.826 kb on - strand at 2818.834 kb on + strand at 2818.842 kb on - strand at 2818.842 kb on - strand at 2818.842 kb on - strand at 2818.853 kb on - strand at 2818.855 kb on + strand at 2818.938 kb on + strand at 2818.941 kb on - strand at 2818.941 kb on - strand at 2818.944 kb on + strand at 2818.944 kb on + strand at 2818.944 kb on + strand at 2818.949 kb on + strand at 2819.052 kb on + strand, within PGA1_c27040 at 2819.079 kb on + strand, within PGA1_c27040 at 2819.087 kb on + strand, within PGA1_c27040 at 2819.130 kb on + strand, within PGA1_c27040 at 2819.181 kb on - strand, within PGA1_c27040 at 2819.226 kb on + strand, within PGA1_c27040 at 2819.229 kb on - strand, within PGA1_c27040 at 2819.248 kb on - strand, within PGA1_c27040 at 2819.252 kb on + strand, within PGA1_c27040 at 2819.269 kb on - strand, within PGA1_c27040 at 2819.288 kb on - strand, within PGA1_c27040 at 2819.449 kb on + strand, within PGA1_c27040 at 2819.455 kb on - strand, within PGA1_c27040 at 2819.485 kb on + strand, within PGA1_c27040 at 2819.490 kb on + strand, within PGA1_c27040 at 2819.493 kb on - strand, within PGA1_c27040 at 2819.493 kb on - strand, within PGA1_c27040 at 2819.495 kb on + strand, within PGA1_c27040 at 2819.503 kb on - strand, within PGA1_c27040 at 2819.531 kb on + strand, within PGA1_c27040 at 2819.551 kb on + strand, within PGA1_c27040 at 2819.551 kb on + strand, within PGA1_c27040 at 2819.559 kb on - strand, within PGA1_c27040 at 2819.600 kb on - strand, within PGA1_c27040 at 2819.633 kb on - strand, within PGA1_c27040 at 2819.741 kb on - strand, within PGA1_c27040 at 2819.799 kb on - strand, within PGA1_c27040 at 2819.859 kb on + strand at 2820.066 kb on + strand at 2820.085 kb on + strand at 2820.112 kb on - strand, within PGA1_c27050 at 2820.114 kb on + strand, within PGA1_c27050 at 2820.149 kb on - strand, within PGA1_c27050 at 2820.298 kb on + strand, within PGA1_c27050 at 2820.298 kb on + strand, within PGA1_c27050 at 2820.306 kb on - strand, within PGA1_c27050 at 2820.306 kb on - strand, within PGA1_c27050 at 2820.306 kb on - strand, within PGA1_c27050 at 2820.306 kb on - strand, within PGA1_c27050 at 2820.306 kb on - strand, within PGA1_c27050 at 2820.306 kb on - strand, within PGA1_c27050 at 2820.368 kb on + strand, within PGA1_c27050 at 2820.403 kb on + strand, within PGA1_c27050 at 2820.411 kb on - strand, within PGA1_c27050 at 2820.418 kb on + strand, within PGA1_c27050 at 2820.426 kb on - strand, within PGA1_c27050 at 2820.432 kb on - strand, within PGA1_c27050 at 2820.542 kb on + strand, within PGA1_c27050 at 2820.594 kb on - strand, within PGA1_c27050 at 2820.594 kb on - strand, within PGA1_c27050 at 2820.603 kb on + strand, within PGA1_c27050 at 2820.611 kb on - strand, within PGA1_c27050 at 2820.642 kb on + strand, within PGA1_c27050 at 2820.645 kb on - strand, within PGA1_c27050 at 2820.655 kb on - strand, within PGA1_c27050 at 2820.660 kb on - strand, within PGA1_c27050 at 2820.669 kb on + strand, within PGA1_c27050 at 2820.804 kb on - strand, within PGA1_c27050
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 2,817,919 - PGA1_c27020 0.58 +1.6 2,818,005 + PGA1_c27020 0.79 +0.6 2,818,064 + +0.0 2,818,064 + +0.0 2,818,072 - -0.4 2,818,081 + +3.0 2,818,089 - +0.8 2,818,089 - +0.6 2,818,103 - +1.6 2,818,135 - -1.4 2,818,137 + -1.2 2,818,145 - +0.0 2,818,145 - -0.4 2,818,147 + -1.2 2,818,155 - +0.6 2,818,155 - -0.4 2,818,212 - PGA1_c27030 0.17 -1.0 2,818,217 + PGA1_c27030 0.18 +1.6 2,818,260 - PGA1_c27030 0.24 +0.6 2,818,331 - PGA1_c27030 0.33 +0.5 2,818,331 - PGA1_c27030 0.33 -0.8 2,818,336 - PGA1_c27030 0.34 +0.0 2,818,336 - PGA1_c27030 0.34 +1.0 2,818,532 + PGA1_c27030 0.61 -0.7 2,818,540 - PGA1_c27030 0.62 +1.6 2,818,558 - PGA1_c27030 0.64 +1.6 2,818,558 - PGA1_c27030 0.64 -1.4 2,818,600 - PGA1_c27030 0.70 -1.4 2,818,608 - PGA1_c27030 0.71 -1.2 2,818,625 + PGA1_c27030 0.73 -0.5 2,818,635 + PGA1_c27030 0.75 -1.7 2,818,777 + +1.6 2,818,777 + +0.0 2,818,785 - +1.6 2,818,807 - +1.6 2,818,818 + -0.7 2,818,826 - -0.7 2,818,834 + +1.6 2,818,842 - +1.6 2,818,842 - +0.3 2,818,842 - +1.6 2,818,853 - +0.6 2,818,855 + +0.2 2,818,938 + -2.4 2,818,941 - +1.6 2,818,941 - +1.6 2,818,944 + +0.4 2,818,944 + -0.1 2,818,944 + -0.4 2,818,949 + +0.0 2,819,052 + PGA1_c27040 0.16 -1.0 2,819,079 + PGA1_c27040 0.19 +1.0 2,819,087 + PGA1_c27040 0.20 -0.8 2,819,130 + PGA1_c27040 0.24 -2.2 2,819,181 - PGA1_c27040 0.29 -0.7 2,819,226 + PGA1_c27040 0.33 -1.2 2,819,229 - PGA1_c27040 0.34 +1.6 2,819,248 - PGA1_c27040 0.35 +0.3 2,819,252 + PGA1_c27040 0.36 +0.6 2,819,269 - PGA1_c27040 0.37 -0.4 2,819,288 - PGA1_c27040 0.39 -1.4 2,819,449 + PGA1_c27040 0.55 +2.2 2,819,455 - PGA1_c27040 0.56 +0.6 2,819,485 + PGA1_c27040 0.59 +0.3 2,819,490 + PGA1_c27040 0.59 -0.4 2,819,493 - PGA1_c27040 0.59 +1.6 2,819,493 - PGA1_c27040 0.59 +0.3 2,819,495 + PGA1_c27040 0.60 -0.7 2,819,503 - PGA1_c27040 0.60 +0.6 2,819,531 + PGA1_c27040 0.63 -0.4 2,819,551 + PGA1_c27040 0.65 -0.4 2,819,551 + PGA1_c27040 0.65 -0.4 2,819,559 - PGA1_c27040 0.66 +0.6 2,819,600 - PGA1_c27040 0.70 -1.0 2,819,633 - PGA1_c27040 0.73 -0.4 2,819,741 - PGA1_c27040 0.84 +0.4 2,819,799 - PGA1_c27040 0.89 +1.0 2,819,859 + +0.7 2,820,066 + -1.4 2,820,085 + -1.0 2,820,112 - PGA1_c27050 0.11 -0.5 2,820,114 + PGA1_c27050 0.11 +1.6 2,820,149 - PGA1_c27050 0.13 +1.6 2,820,298 + PGA1_c27050 0.23 +0.7 2,820,298 + PGA1_c27050 0.23 +0.9 2,820,306 - PGA1_c27050 0.23 +0.6 2,820,306 - PGA1_c27050 0.23 +0.9 2,820,306 - PGA1_c27050 0.23 -1.2 2,820,306 - PGA1_c27050 0.23 -0.2 2,820,306 - PGA1_c27050 0.23 -0.2 2,820,306 - PGA1_c27050 0.23 +0.0 2,820,368 + PGA1_c27050 0.28 +1.1 2,820,403 + PGA1_c27050 0.30 -0.1 2,820,411 - PGA1_c27050 0.30 -1.0 2,820,418 + PGA1_c27050 0.31 -0.4 2,820,426 - PGA1_c27050 0.31 -1.4 2,820,432 - PGA1_c27050 0.32 -0.4 2,820,542 + PGA1_c27050 0.39 +0.6 2,820,594 - PGA1_c27050 0.42 +1.6 2,820,594 - PGA1_c27050 0.42 +1.6 2,820,603 + PGA1_c27050 0.43 +0.6 2,820,611 - PGA1_c27050 0.44 -0.4 2,820,642 + PGA1_c27050 0.46 -0.0 2,820,645 - PGA1_c27050 0.46 -1.2 2,820,655 - PGA1_c27050 0.46 +1.6 2,820,660 - PGA1_c27050 0.47 -1.5 2,820,669 + PGA1_c27050 0.47 +0.0 2,820,804 - PGA1_c27050 0.56 +0.6
Or see this region's nucleotide sequence