Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24290 and PGA1_c24300 overlap by 4 nucleotides PGA1_c24300 and PGA1_c24310 are separated by 291 nucleotides PGA1_c24310 and PGA1_c24320 overlap by 23 nucleotides
PGA1_c24290: PGA1_c24290 - hypothetical protein, at 2,534,482 to 2,535,279
_c24290
PGA1_c24300: PGA1_c24300 - Uracil-DNA glycosylase, at 2,535,276 to 2,535,869
_c24300
PGA1_c24310: PGA1_c24310 - putative capsular polysaccharide transport system, ATP-binding protein, at 2,536,161 to 2,536,820
_c24310
PGA1_c24320: PGA1_c24320 - putative capsule polysaccharide export inner-membrane protein, at 2,536,798 to 2,538,504
_c24320
Position (kb)
2536
2537 Strain fitness (log2 ratio)
-1
0
1
2 at 2535.228 kb on - strand at 2535.285 kb on - strand at 2535.298 kb on - strand at 2535.343 kb on + strand, within PGA1_c24300 at 2535.400 kb on + strand, within PGA1_c24300 at 2535.432 kb on + strand, within PGA1_c24300 at 2535.472 kb on + strand, within PGA1_c24300 at 2535.491 kb on + strand, within PGA1_c24300 at 2535.550 kb on + strand, within PGA1_c24300 at 2535.645 kb on + strand, within PGA1_c24300 at 2535.676 kb on - strand, within PGA1_c24300 at 2535.683 kb on + strand, within PGA1_c24300 at 2535.696 kb on - strand, within PGA1_c24300 at 2535.773 kb on - strand, within PGA1_c24300 at 2535.773 kb on - strand, within PGA1_c24300 at 2535.799 kb on - strand, within PGA1_c24300 at 2535.799 kb on - strand, within PGA1_c24300 at 2535.812 kb on + strand at 2535.817 kb on + strand at 2535.869 kb on + strand at 2535.987 kb on + strand at 2536.040 kb on - strand at 2536.053 kb on - strand at 2536.078 kb on + strand at 2536.078 kb on + strand at 2536.085 kb on - strand at 2536.086 kb on - strand at 2536.100 kb on - strand at 2536.101 kb on - strand at 2536.115 kb on + strand at 2536.122 kb on - strand at 2536.123 kb on - strand at 2536.124 kb on + strand at 2536.124 kb on + strand at 2536.134 kb on - strand at 2536.136 kb on + strand at 2536.136 kb on + strand at 2536.144 kb on - strand at 2536.144 kb on - strand at 2536.144 kb on - strand at 2536.159 kb on + strand at 2536.240 kb on + strand, within PGA1_c24310 at 2536.248 kb on - strand, within PGA1_c24310 at 2536.248 kb on - strand, within PGA1_c24310 at 2536.294 kb on - strand, within PGA1_c24310 at 2536.296 kb on + strand, within PGA1_c24310 at 2536.315 kb on - strand, within PGA1_c24310 at 2536.365 kb on - strand, within PGA1_c24310 at 2536.415 kb on + strand, within PGA1_c24310 at 2536.512 kb on - strand, within PGA1_c24310 at 2536.512 kb on - strand, within PGA1_c24310 at 2536.512 kb on - strand, within PGA1_c24310 at 2536.550 kb on + strand, within PGA1_c24310 at 2536.558 kb on - strand, within PGA1_c24310 at 2536.558 kb on - strand, within PGA1_c24310 at 2536.610 kb on + strand, within PGA1_c24310 at 2536.675 kb on - strand, within PGA1_c24310 at 2536.703 kb on + strand, within PGA1_c24310 at 2536.788 kb on + strand at 2536.793 kb on + strand at 2536.793 kb on + strand at 2536.793 kb on + strand at 2536.875 kb on - strand at 2536.895 kb on + strand at 2536.895 kb on + strand at 2537.049 kb on + strand, within PGA1_c24320 at 2537.060 kb on + strand, within PGA1_c24320 at 2537.060 kb on + strand, within PGA1_c24320 at 2537.063 kb on - strand, within PGA1_c24320 at 2537.143 kb on + strand, within PGA1_c24320 at 2537.185 kb on - strand, within PGA1_c24320 at 2537.192 kb on + strand, within PGA1_c24320 at 2537.192 kb on + strand, within PGA1_c24320 at 2537.200 kb on - strand, within PGA1_c24320 at 2537.238 kb on + strand, within PGA1_c24320 at 2537.238 kb on + strand, within PGA1_c24320 at 2537.246 kb on - strand, within PGA1_c24320 at 2537.282 kb on + strand, within PGA1_c24320 at 2537.499 kb on + strand, within PGA1_c24320 at 2537.537 kb on - strand, within PGA1_c24320 at 2537.574 kb on + strand, within PGA1_c24320 at 2537.612 kb on - strand, within PGA1_c24320 at 2537.625 kb on - strand, within PGA1_c24320 at 2537.694 kb on + strand, within PGA1_c24320 at 2537.702 kb on - strand, within PGA1_c24320 at 2537.703 kb on - strand, within PGA1_c24320 at 2537.707 kb on - strand, within PGA1_c24320 at 2537.707 kb on - strand, within PGA1_c24320 at 2537.724 kb on + strand, within PGA1_c24320 at 2537.805 kb on - strand, within PGA1_c24320
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 2,535,228 - +0.0 2,535,285 - -0.2 2,535,298 - +2.6 2,535,343 + PGA1_c24300 0.11 -0.4 2,535,400 + PGA1_c24300 0.21 +1.0 2,535,432 + PGA1_c24300 0.26 +0.6 2,535,472 + PGA1_c24300 0.33 +0.2 2,535,491 + PGA1_c24300 0.36 -0.7 2,535,550 + PGA1_c24300 0.46 +0.6 2,535,645 + PGA1_c24300 0.62 -1.7 2,535,676 - PGA1_c24300 0.67 +0.6 2,535,683 + PGA1_c24300 0.69 +0.0 2,535,696 - PGA1_c24300 0.71 +1.6 2,535,773 - PGA1_c24300 0.84 +1.6 2,535,773 - PGA1_c24300 0.84 +0.6 2,535,799 - PGA1_c24300 0.88 +2.2 2,535,799 - PGA1_c24300 0.88 +1.9 2,535,812 + -0.7 2,535,817 + +0.0 2,535,869 + +1.3 2,535,987 + -1.0 2,536,040 - -1.0 2,536,053 - -0.4 2,536,078 + +2.6 2,536,078 + +0.9 2,536,085 - +0.6 2,536,086 - +0.6 2,536,100 - +1.6 2,536,101 - +0.6 2,536,115 + -1.7 2,536,122 - +0.6 2,536,123 - +1.6 2,536,124 + +0.0 2,536,124 + +1.6 2,536,134 - +0.9 2,536,136 + -1.7 2,536,136 + +1.6 2,536,144 - +0.0 2,536,144 - +0.9 2,536,144 - -0.4 2,536,159 + +1.6 2,536,240 + PGA1_c24310 0.12 +0.0 2,536,248 - PGA1_c24310 0.13 -1.2 2,536,248 - PGA1_c24310 0.13 +1.6 2,536,294 - PGA1_c24310 0.20 +0.0 2,536,296 + PGA1_c24310 0.20 -1.0 2,536,315 - PGA1_c24310 0.23 +0.6 2,536,365 - PGA1_c24310 0.31 -0.7 2,536,415 + PGA1_c24310 0.38 +0.6 2,536,512 - PGA1_c24310 0.53 +1.6 2,536,512 - PGA1_c24310 0.53 -0.4 2,536,512 - PGA1_c24310 0.53 +0.6 2,536,550 + PGA1_c24310 0.59 +0.6 2,536,558 - PGA1_c24310 0.60 -0.7 2,536,558 - PGA1_c24310 0.60 +0.2 2,536,610 + PGA1_c24310 0.68 +1.2 2,536,675 - PGA1_c24310 0.78 -0.7 2,536,703 + PGA1_c24310 0.82 -0.6 2,536,788 + +0.0 2,536,793 + -1.2 2,536,793 + -1.4 2,536,793 + -0.4 2,536,875 - +1.6 2,536,895 + +0.3 2,536,895 + -1.0 2,537,049 + PGA1_c24320 0.15 -0.6 2,537,060 + PGA1_c24320 0.15 +0.6 2,537,060 + PGA1_c24320 0.15 +1.0 2,537,063 - PGA1_c24320 0.16 -0.4 2,537,143 + PGA1_c24320 0.20 -0.9 2,537,185 - PGA1_c24320 0.23 -0.4 2,537,192 + PGA1_c24320 0.23 +0.3 2,537,192 + PGA1_c24320 0.23 +2.6 2,537,200 - PGA1_c24320 0.24 +0.0 2,537,238 + PGA1_c24320 0.26 -1.2 2,537,238 + PGA1_c24320 0.26 -0.2 2,537,246 - PGA1_c24320 0.26 +0.6 2,537,282 + PGA1_c24320 0.28 -1.6 2,537,499 + PGA1_c24320 0.41 +1.6 2,537,537 - PGA1_c24320 0.43 +0.0 2,537,574 + PGA1_c24320 0.45 +0.6 2,537,612 - PGA1_c24320 0.48 -0.7 2,537,625 - PGA1_c24320 0.48 +0.6 2,537,694 + PGA1_c24320 0.52 -1.0 2,537,702 - PGA1_c24320 0.53 -1.4 2,537,703 - PGA1_c24320 0.53 +0.6 2,537,707 - PGA1_c24320 0.53 +1.6 2,537,707 - PGA1_c24320 0.53 +1.6 2,537,724 + PGA1_c24320 0.54 -1.6 2,537,805 - PGA1_c24320 0.59 -0.7
Or see this region's nucleotide sequence