Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22990 and PGA1_c23000 are separated by 174 nucleotides PGA1_c23000 and PGA1_c23010 overlap by 14 nucleotides PGA1_c23010 and PGA1_c23020 overlap by 4 nucleotides PGA1_c23020 and PGA1_c23030 overlap by 4 nucleotides PGA1_c23030 and PGA1_c23040 are separated by 744 nucleotides
PGA1_c22990: PGA1_c22990 - Bacterial mobilisation protein (MobC)., at 2,384,797 to 2,385,387
_c22990
PGA1_c23000: PGA1_c23000 - hypothetical protein, at 2,385,562 to 2,385,843
_c23000
PGA1_c23010: PGA1_c23010 - hypothetical protein, at 2,385,830 to 2,386,054
_c23010
PGA1_c23020: PGA1_c23020 - hypothetical protein, at 2,386,051 to 2,386,254
_c23020
PGA1_c23030: PGA1_c23030 - putative ArdC antirestriction protein, at 2,386,251 to 2,387,129
_c23030
PGA1_c23040: PGA1_c23040 - Uncharacterized protein conserved in bacteria, at 2,387,874 to 2,388,578
_c23040
Position (kb)
2386
2387
2388 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2385.286 kb on + strand, within PGA1_c22990 at 2385.376 kb on + strand at 2385.514 kb on - strand at 2385.549 kb on + strand at 2385.552 kb on - strand at 2385.552 kb on - strand at 2385.559 kb on - strand at 2385.571 kb on + strand at 2385.686 kb on - strand, within PGA1_c23000 at 2385.740 kb on - strand, within PGA1_c23000 at 2385.740 kb on - strand, within PGA1_c23000 at 2385.850 kb on + strand at 2385.858 kb on - strand, within PGA1_c23010 at 2385.858 kb on - strand, within PGA1_c23010 at 2385.858 kb on - strand, within PGA1_c23010 at 2385.890 kb on + strand, within PGA1_c23010 at 2385.914 kb on + strand, within PGA1_c23010 at 2385.922 kb on - strand, within PGA1_c23010 at 2385.961 kb on + strand, within PGA1_c23010 at 2385.961 kb on + strand, within PGA1_c23010 at 2385.961 kb on + strand, within PGA1_c23010 at 2385.961 kb on + strand, within PGA1_c23010 at 2385.969 kb on - strand, within PGA1_c23010 at 2385.969 kb on - strand, within PGA1_c23010 at 2385.969 kb on - strand, within PGA1_c23010 at 2386.016 kb on + strand, within PGA1_c23010 at 2386.042 kb on + strand at 2386.049 kb on + strand at 2386.050 kb on - strand at 2386.052 kb on + strand at 2386.072 kb on - strand, within PGA1_c23020 at 2386.083 kb on + strand, within PGA1_c23020 at 2386.083 kb on + strand, within PGA1_c23020 at 2386.091 kb on - strand, within PGA1_c23020 at 2386.091 kb on - strand, within PGA1_c23020 at 2386.091 kb on - strand, within PGA1_c23020 at 2386.091 kb on - strand, within PGA1_c23020 at 2386.091 kb on - strand, within PGA1_c23020 at 2386.108 kb on - strand, within PGA1_c23020 at 2386.110 kb on + strand, within PGA1_c23020 at 2386.110 kb on + strand, within PGA1_c23020 at 2386.118 kb on - strand, within PGA1_c23020 at 2386.144 kb on + strand, within PGA1_c23020 at 2386.144 kb on + strand, within PGA1_c23020 at 2386.152 kb on - strand, within PGA1_c23020 at 2386.152 kb on - strand, within PGA1_c23020 at 2386.152 kb on - strand, within PGA1_c23020 at 2386.152 kb on - strand, within PGA1_c23020 at 2386.152 kb on - strand, within PGA1_c23020 at 2386.152 kb on - strand, within PGA1_c23020 at 2386.202 kb on - strand, within PGA1_c23020 at 2386.269 kb on + strand at 2386.269 kb on + strand at 2386.269 kb on + strand at 2386.269 kb on + strand at 2386.274 kb on + strand at 2386.274 kb on + strand at 2386.277 kb on - strand at 2386.277 kb on - strand at 2386.277 kb on - strand at 2386.277 kb on - strand at 2386.277 kb on - strand at 2386.277 kb on - strand at 2386.282 kb on - strand at 2386.349 kb on + strand, within PGA1_c23030 at 2386.357 kb on - strand, within PGA1_c23030 at 2386.366 kb on - strand, within PGA1_c23030 at 2386.366 kb on - strand, within PGA1_c23030 at 2386.366 kb on - strand, within PGA1_c23030 at 2386.408 kb on + strand, within PGA1_c23030 at 2386.413 kb on + strand, within PGA1_c23030 at 2386.416 kb on - strand, within PGA1_c23030 at 2386.424 kb on - strand, within PGA1_c23030 at 2386.457 kb on + strand, within PGA1_c23030 at 2386.464 kb on + strand, within PGA1_c23030 at 2386.464 kb on + strand, within PGA1_c23030 at 2386.464 kb on + strand, within PGA1_c23030 at 2386.472 kb on - strand, within PGA1_c23030 at 2386.472 kb on - strand, within PGA1_c23030 at 2386.525 kb on + strand, within PGA1_c23030 at 2386.543 kb on - strand, within PGA1_c23030 at 2386.562 kb on - strand, within PGA1_c23030 at 2386.562 kb on - strand, within PGA1_c23030 at 2386.572 kb on + strand, within PGA1_c23030 at 2386.607 kb on - strand, within PGA1_c23030 at 2386.642 kb on - strand, within PGA1_c23030 at 2386.752 kb on + strand, within PGA1_c23030 at 2386.761 kb on - strand, within PGA1_c23030 at 2386.866 kb on + strand, within PGA1_c23030 at 2386.866 kb on + strand, within PGA1_c23030 at 2386.874 kb on - strand, within PGA1_c23030 at 2386.881 kb on - strand, within PGA1_c23030 at 2386.890 kb on + strand, within PGA1_c23030 at 2386.895 kb on + strand, within PGA1_c23030 at 2386.895 kb on + strand, within PGA1_c23030 at 2386.903 kb on - strand, within PGA1_c23030 at 2386.903 kb on - strand, within PGA1_c23030 at 2386.903 kb on - strand, within PGA1_c23030 at 2386.915 kb on - strand, within PGA1_c23030 at 2387.004 kb on - strand, within PGA1_c23030 at 2387.046 kb on - strand at 2387.052 kb on + strand at 2387.068 kb on + strand at 2387.076 kb on - strand at 2387.076 kb on - strand at 2387.119 kb on - strand at 2387.182 kb on + strand at 2387.188 kb on + strand at 2387.195 kb on - strand at 2387.202 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.241 kb on + strand at 2387.249 kb on - strand at 2387.249 kb on - strand at 2387.249 kb on - strand at 2387.249 kb on - strand at 2387.249 kb on - strand at 2387.258 kb on + strand at 2387.259 kb on - strand at 2387.260 kb on - strand at 2387.265 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.270 kb on + strand at 2387.275 kb on + strand at 2387.275 kb on + strand at 2387.275 kb on + strand at 2387.275 kb on + strand at 2387.275 kb on + strand at 2387.275 kb on + strand at 2387.275 kb on + strand at 2387.278 kb on - strand at 2387.278 kb on - strand at 2387.278 kb on - strand at 2387.280 kb on - strand at 2387.356 kb on - strand at 2387.357 kb on - strand at 2387.358 kb on - strand at 2387.399 kb on + strand at 2387.400 kb on + strand at 2387.400 kb on + strand at 2387.400 kb on + strand at 2387.400 kb on + strand at 2387.408 kb on - strand at 2387.408 kb on - strand at 2387.408 kb on - strand at 2387.408 kb on - strand at 2387.408 kb on - strand at 2387.408 kb on - strand at 2387.408 kb on - strand at 2387.429 kb on - strand at 2387.461 kb on + strand at 2387.461 kb on + strand at 2387.469 kb on - strand at 2387.469 kb on - strand at 2387.476 kb on + strand at 2387.476 kb on + strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.484 kb on - strand at 2387.494 kb on + strand at 2387.535 kb on + strand at 2387.601 kb on + strand at 2387.609 kb on - strand at 2387.624 kb on - strand at 2387.626 kb on + strand at 2387.689 kb on - strand at 2387.725 kb on + strand at 2387.725 kb on + strand at 2387.743 kb on - strand at 2387.788 kb on - strand at 2387.788 kb on - strand at 2387.792 kb on + strand at 2387.793 kb on - strand at 2387.829 kb on + strand at 2387.847 kb on + strand at 2387.849 kb on + strand at 2387.855 kb on - strand at 2387.864 kb on + strand at 2387.872 kb on - strand at 2387.898 kb on - strand at 2387.941 kb on - strand at 2387.956 kb on - strand, within PGA1_c23040 at 2387.973 kb on - strand, within PGA1_c23040 at 2387.975 kb on - strand, within PGA1_c23040 at 2387.975 kb on - strand, within PGA1_c23040 at 2388.003 kb on + strand, within PGA1_c23040 at 2388.012 kb on + strand, within PGA1_c23040 at 2388.023 kb on + strand, within PGA1_c23040 at 2388.023 kb on + strand, within PGA1_c23040 at 2388.031 kb on + strand, within PGA1_c23040 at 2388.075 kb on + strand, within PGA1_c23040
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 2,385,286 + PGA1_c22990 0.83 -1.3 2,385,376 + -0.4 2,385,514 - -0.4 2,385,549 + +0.8 2,385,552 - +2.0 2,385,552 - -0.0 2,385,559 - +0.4 2,385,571 + +1.7 2,385,686 - PGA1_c23000 0.44 +1.1 2,385,740 - PGA1_c23000 0.63 -2.7 2,385,740 - PGA1_c23000 0.63 +1.3 2,385,850 + +0.1 2,385,858 - PGA1_c23010 0.12 +1.3 2,385,858 - PGA1_c23010 0.12 -1.0 2,385,858 - PGA1_c23010 0.12 -1.6 2,385,890 + PGA1_c23010 0.27 +0.8 2,385,914 + PGA1_c23010 0.37 -0.3 2,385,922 - PGA1_c23010 0.41 -1.0 2,385,961 + PGA1_c23010 0.58 +0.6 2,385,961 + PGA1_c23010 0.58 -0.4 2,385,961 + PGA1_c23010 0.58 -0.4 2,385,961 + PGA1_c23010 0.58 +1.6 2,385,969 - PGA1_c23010 0.62 +0.3 2,385,969 - PGA1_c23010 0.62 -1.3 2,385,969 - PGA1_c23010 0.62 +1.6 2,386,016 + PGA1_c23010 0.83 -1.1 2,386,042 + +0.2 2,386,049 + -0.4 2,386,050 - +1.6 2,386,052 + -1.0 2,386,072 - PGA1_c23020 0.10 +0.7 2,386,083 + PGA1_c23020 0.16 -0.5 2,386,083 + PGA1_c23020 0.16 +0.2 2,386,091 - PGA1_c23020 0.20 +0.2 2,386,091 - PGA1_c23020 0.20 -1.8 2,386,091 - PGA1_c23020 0.20 -0.0 2,386,091 - PGA1_c23020 0.20 +1.2 2,386,091 - PGA1_c23020 0.20 -0.6 2,386,108 - PGA1_c23020 0.28 -1.0 2,386,110 + PGA1_c23020 0.29 -1.0 2,386,110 + PGA1_c23020 0.29 -0.3 2,386,118 - PGA1_c23020 0.33 +1.0 2,386,144 + PGA1_c23020 0.46 -0.0 2,386,144 + PGA1_c23020 0.46 -2.7 2,386,152 - PGA1_c23020 0.50 -0.3 2,386,152 - PGA1_c23020 0.50 -1.8 2,386,152 - PGA1_c23020 0.50 -1.5 2,386,152 - PGA1_c23020 0.50 +0.5 2,386,152 - PGA1_c23020 0.50 -0.0 2,386,152 - PGA1_c23020 0.50 -0.5 2,386,202 - PGA1_c23020 0.74 +0.1 2,386,269 + +0.1 2,386,269 + -1.4 2,386,269 + +0.1 2,386,269 + -0.5 2,386,274 + +0.9 2,386,274 + +0.2 2,386,277 - -0.8 2,386,277 - -0.9 2,386,277 - +1.3 2,386,277 - -0.1 2,386,277 - +0.1 2,386,277 - +0.1 2,386,282 - +0.4 2,386,349 + PGA1_c23030 0.11 -1.4 2,386,357 - PGA1_c23030 0.12 -1.5 2,386,366 - PGA1_c23030 0.13 -1.9 2,386,366 - PGA1_c23030 0.13 -0.6 2,386,366 - PGA1_c23030 0.13 -0.0 2,386,408 + PGA1_c23030 0.18 +0.1 2,386,413 + PGA1_c23030 0.18 +0.7 2,386,416 - PGA1_c23030 0.19 +0.2 2,386,424 - PGA1_c23030 0.20 +1.6 2,386,457 + PGA1_c23030 0.23 -2.0 2,386,464 + PGA1_c23030 0.24 +1.2 2,386,464 + PGA1_c23030 0.24 +0.6 2,386,464 + PGA1_c23030 0.24 -1.3 2,386,472 - PGA1_c23030 0.25 +0.6 2,386,472 - PGA1_c23030 0.25 -0.2 2,386,525 + PGA1_c23030 0.31 +1.1 2,386,543 - PGA1_c23030 0.33 -0.8 2,386,562 - PGA1_c23030 0.35 +0.8 2,386,562 - PGA1_c23030 0.35 -1.8 2,386,572 + PGA1_c23030 0.37 +2.6 2,386,607 - PGA1_c23030 0.41 +0.5 2,386,642 - PGA1_c23030 0.44 +0.1 2,386,752 + PGA1_c23030 0.57 +1.1 2,386,761 - PGA1_c23030 0.58 -1.3 2,386,866 + PGA1_c23030 0.70 +0.1 2,386,866 + PGA1_c23030 0.70 +1.4 2,386,874 - PGA1_c23030 0.71 -0.8 2,386,881 - PGA1_c23030 0.72 -0.4 2,386,890 + PGA1_c23030 0.73 -1.3 2,386,895 + PGA1_c23030 0.73 +0.6 2,386,895 + PGA1_c23030 0.73 -0.3 2,386,903 - PGA1_c23030 0.74 +0.6 2,386,903 - PGA1_c23030 0.74 +1.0 2,386,903 - PGA1_c23030 0.74 +1.0 2,386,915 - PGA1_c23030 0.76 +0.2 2,387,004 - PGA1_c23030 0.86 -1.0 2,387,046 - -1.4 2,387,052 + -1.8 2,387,068 + -0.0 2,387,076 - +0.3 2,387,076 - +0.9 2,387,119 - +1.0 2,387,182 + +1.0 2,387,188 + +0.6 2,387,195 - +0.6 2,387,202 + -0.1 2,387,241 + +0.1 2,387,241 + +1.0 2,387,241 + -0.9 2,387,241 + +1.2 2,387,241 + +0.8 2,387,241 + +0.7 2,387,241 + -0.4 2,387,241 + -0.8 2,387,241 + -0.3 2,387,249 - -0.0 2,387,249 - -0.1 2,387,249 - -1.6 2,387,249 - -0.2 2,387,249 - +0.7 2,387,258 + +0.9 2,387,259 - +0.7 2,387,260 - +0.6 2,387,265 + -0.4 2,387,270 + +1.0 2,387,270 + +0.1 2,387,270 + -0.3 2,387,270 + +0.1 2,387,270 + -1.0 2,387,270 + -0.5 2,387,270 + +0.3 2,387,270 + -1.3 2,387,270 + +0.5 2,387,270 + -0.0 2,387,270 + -0.1 2,387,275 + +0.9 2,387,275 + +0.7 2,387,275 + +1.6 2,387,275 + -1.6 2,387,275 + -2.0 2,387,275 + +0.2 2,387,275 + -0.0 2,387,278 - -2.4 2,387,278 - -0.6 2,387,278 - -0.4 2,387,280 - -1.3 2,387,356 - +0.6 2,387,357 - +0.1 2,387,358 - -0.0 2,387,399 + -1.4 2,387,400 + -0.5 2,387,400 + -0.4 2,387,400 + -1.4 2,387,400 + -0.4 2,387,408 - +0.4 2,387,408 - +1.6 2,387,408 - +0.2 2,387,408 - -0.8 2,387,408 - -0.3 2,387,408 - +0.1 2,387,408 - -0.6 2,387,429 - +0.3 2,387,461 + -0.0 2,387,461 + -0.6 2,387,469 - -0.3 2,387,469 - +0.5 2,387,476 + +1.6 2,387,476 + +2.3 2,387,484 - +1.3 2,387,484 - -1.6 2,387,484 - +0.2 2,387,484 - +1.8 2,387,484 - -0.7 2,387,484 - -0.3 2,387,484 - -0.2 2,387,484 - -0.1 2,387,494 + +0.7 2,387,535 + +0.4 2,387,601 + -3.4 2,387,609 - +0.7 2,387,624 - -0.0 2,387,626 + +1.2 2,387,689 - -0.7 2,387,725 + -0.9 2,387,725 + -1.2 2,387,743 - -1.0 2,387,788 - -0.8 2,387,788 - +0.8 2,387,792 + +0.8 2,387,793 - -0.7 2,387,829 + +0.6 2,387,847 + -0.4 2,387,849 + -0.3 2,387,855 - +0.6 2,387,864 + +0.9 2,387,872 - +0.2 2,387,898 - -0.9 2,387,941 - +0.6 2,387,956 - PGA1_c23040 0.12 +1.0 2,387,973 - PGA1_c23040 0.14 +2.0 2,387,975 - PGA1_c23040 0.14 -0.2 2,387,975 - PGA1_c23040 0.14 +0.6 2,388,003 + PGA1_c23040 0.18 +0.6 2,388,012 + PGA1_c23040 0.20 -1.3 2,388,023 + PGA1_c23040 0.21 -1.2 2,388,023 + PGA1_c23040 0.21 -0.2 2,388,031 + PGA1_c23040 0.22 -0.0 2,388,075 + PGA1_c23040 0.29 +0.9
Or see this region's nucleotide sequence