Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22000 and PGA1_c22010 are separated by 74 nucleotides PGA1_c22010 and PGA1_c22020 are separated by 62 nucleotides PGA1_c22020 and PGA1_c22030 are separated by 190 nucleotides
PGA1_c22000: PGA1_c22000 - putative DNA-3-methyladenine glycosylase 1, at 2,280,814 to 2,281,437
_c22000
PGA1_c22010: PGA1_c22010 - tRNA-specific adenosine deaminase TadA, at 2,281,512 to 2,281,973
_c22010
PGA1_c22020: PGA1_c22020 - putative ribosomal large subunit pseudouridine synthase B, at 2,282,036 to 2,283,361
_c22020
PGA1_c22030: PGA1_c22030 - 5-bromo-4-chloroindolyl phosphate hydrolysis protein., at 2,283,552 to 2,284,469
_c22030
Position (kb)
2282
2283
2284 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2281.055 kb on - strand, within PGA1_c22000 at 2281.055 kb on - strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.091 kb on + strand, within PGA1_c22000 at 2281.099 kb on - strand, within PGA1_c22000 at 2281.099 kb on - strand, within PGA1_c22000 at 2281.099 kb on - strand, within PGA1_c22000 at 2281.115 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.273 kb on + strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.281 kb on - strand, within PGA1_c22000 at 2281.298 kb on + strand, within PGA1_c22000 at 2281.372 kb on - strand, within PGA1_c22000 at 2281.475 kb on + strand at 2281.523 kb on - strand at 2281.523 kb on - strand at 2281.970 kb on + strand at 2281.974 kb on + strand at 2281.985 kb on + strand at 2282.022 kb on + strand at 2282.080 kb on + strand at 2282.093 kb on - strand at 2282.161 kb on + strand at 2282.267 kb on + strand, within PGA1_c22020 at 2282.297 kb on - strand, within PGA1_c22020 at 2282.348 kb on - strand, within PGA1_c22020 at 2282.374 kb on + strand, within PGA1_c22020 at 2282.382 kb on - strand, within PGA1_c22020 at 2282.389 kb on - strand, within PGA1_c22020 at 2282.550 kb on - strand, within PGA1_c22020 at 2282.550 kb on - strand, within PGA1_c22020 at 2282.589 kb on + strand, within PGA1_c22020 at 2282.693 kb on - strand, within PGA1_c22020 at 2282.711 kb on - strand, within PGA1_c22020 at 2282.788 kb on + strand, within PGA1_c22020 at 2282.837 kb on + strand, within PGA1_c22020 at 2282.860 kb on + strand, within PGA1_c22020 at 2282.874 kb on - strand, within PGA1_c22020 at 2282.938 kb on + strand, within PGA1_c22020 at 2282.939 kb on + strand, within PGA1_c22020 at 2283.122 kb on + strand, within PGA1_c22020 at 2283.178 kb on - strand, within PGA1_c22020 at 2283.198 kb on + strand, within PGA1_c22020 at 2283.206 kb on - strand, within PGA1_c22020 at 2283.307 kb on + strand at 2283.355 kb on + strand at 2283.355 kb on + strand at 2283.542 kb on + strand at 2283.542 kb on + strand at 2283.550 kb on + strand at 2283.580 kb on + strand at 2283.580 kb on + strand at 2283.588 kb on - strand at 2283.619 kb on + strand at 2283.622 kb on - strand at 2283.658 kb on + strand, within PGA1_c22030 at 2283.675 kb on + strand, within PGA1_c22030 at 2283.705 kb on + strand, within PGA1_c22030 at 2283.813 kb on + strand, within PGA1_c22030 at 2283.893 kb on + strand, within PGA1_c22030 at 2283.901 kb on - strand, within PGA1_c22030 at 2283.901 kb on - strand, within PGA1_c22030 at 2283.956 kb on + strand, within PGA1_c22030 at 2283.956 kb on + strand, within PGA1_c22030 at 2284.106 kb on - strand, within PGA1_c22030 at 2284.149 kb on + strand, within PGA1_c22030 at 2284.181 kb on + strand, within PGA1_c22030 at 2284.210 kb on - strand, within PGA1_c22030 at 2284.226 kb on + strand, within PGA1_c22030 at 2284.284 kb on + strand, within PGA1_c22030 at 2284.352 kb on + strand, within PGA1_c22030
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 2,281,055 - PGA1_c22000 0.39 -0.3 2,281,055 - PGA1_c22000 0.39 -1.0 2,281,091 + PGA1_c22000 0.44 -0.4 2,281,091 + PGA1_c22000 0.44 +1.5 2,281,091 + PGA1_c22000 0.44 +0.8 2,281,091 + PGA1_c22000 0.44 +1.5 2,281,099 - PGA1_c22000 0.46 -1.3 2,281,099 - PGA1_c22000 0.46 +0.5 2,281,099 - PGA1_c22000 0.46 -0.2 2,281,115 + PGA1_c22000 0.48 +0.7 2,281,273 + PGA1_c22000 0.74 +0.9 2,281,273 + PGA1_c22000 0.74 +0.5 2,281,273 + PGA1_c22000 0.74 +0.9 2,281,273 + PGA1_c22000 0.74 +0.3 2,281,273 + PGA1_c22000 0.74 +1.5 2,281,273 + PGA1_c22000 0.74 +0.9 2,281,273 + PGA1_c22000 0.74 +1.3 2,281,273 + PGA1_c22000 0.74 +1.2 2,281,273 + PGA1_c22000 0.74 +0.1 2,281,273 + PGA1_c22000 0.74 -0.8 2,281,281 - PGA1_c22000 0.75 +1.1 2,281,281 - PGA1_c22000 0.75 +1.2 2,281,281 - PGA1_c22000 0.75 +0.0 2,281,281 - PGA1_c22000 0.75 +0.8 2,281,281 - PGA1_c22000 0.75 +0.2 2,281,281 - PGA1_c22000 0.75 -1.3 2,281,281 - PGA1_c22000 0.75 -1.4 2,281,298 + PGA1_c22000 0.78 +0.5 2,281,372 - PGA1_c22000 0.89 -1.1 2,281,475 + +1.5 2,281,523 - -0.5 2,281,523 - +2.5 2,281,970 + +0.9 2,281,974 + -1.5 2,281,985 + -0.2 2,282,022 + +1.5 2,282,080 + -0.5 2,282,093 - +0.2 2,282,161 + +0.3 2,282,267 + PGA1_c22020 0.17 -0.3 2,282,297 - PGA1_c22020 0.20 -1.3 2,282,348 - PGA1_c22020 0.24 -0.1 2,282,374 + PGA1_c22020 0.25 +1.5 2,282,382 - PGA1_c22020 0.26 -0.3 2,282,389 - PGA1_c22020 0.27 +2.5 2,282,550 - PGA1_c22020 0.39 -0.3 2,282,550 - PGA1_c22020 0.39 +1.7 2,282,589 + PGA1_c22020 0.42 -1.5 2,282,693 - PGA1_c22020 0.50 +1.5 2,282,711 - PGA1_c22020 0.51 -0.1 2,282,788 + PGA1_c22020 0.57 -2.3 2,282,837 + PGA1_c22020 0.60 -0.1 2,282,860 + PGA1_c22020 0.62 -0.1 2,282,874 - PGA1_c22020 0.63 -1.1 2,282,938 + PGA1_c22020 0.68 -0.8 2,282,939 + PGA1_c22020 0.68 +0.9 2,283,122 + PGA1_c22020 0.82 -0.3 2,283,178 - PGA1_c22020 0.86 +0.1 2,283,198 + PGA1_c22020 0.88 -1.5 2,283,206 - PGA1_c22020 0.88 -1.1 2,283,307 + -0.8 2,283,355 + -0.2 2,283,355 + +0.9 2,283,542 + +1.8 2,283,542 + +0.8 2,283,550 + +1.8 2,283,580 + +1.9 2,283,580 + +0.9 2,283,588 - -1.8 2,283,619 + -0.1 2,283,622 - -2.4 2,283,658 + PGA1_c22030 0.12 -1.2 2,283,675 + PGA1_c22030 0.13 -0.5 2,283,705 + PGA1_c22030 0.17 -0.8 2,283,813 + PGA1_c22030 0.28 -2.1 2,283,893 + PGA1_c22030 0.37 +0.2 2,283,901 - PGA1_c22030 0.38 -0.5 2,283,901 - PGA1_c22030 0.38 +1.5 2,283,956 + PGA1_c22030 0.44 -1.3 2,283,956 + PGA1_c22030 0.44 -1.5 2,284,106 - PGA1_c22030 0.60 -0.1 2,284,149 + PGA1_c22030 0.65 -0.5 2,284,181 + PGA1_c22030 0.69 -0.5 2,284,210 - PGA1_c22030 0.72 -0.5 2,284,226 + PGA1_c22030 0.73 -1.1 2,284,284 + PGA1_c22030 0.80 -1.5 2,284,352 + PGA1_c22030 0.87 -0.6
Or see this region's nucleotide sequence