Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c19060 and PGA1_c19070 are separated by 135 nucleotides PGA1_c19070 and PGA1_c19080 are separated by 165 nucleotides PGA1_c19080 and PGA1_c19090 are separated by 112 nucleotides
PGA1_c19060: PGA1_c19060 - ABC transporter, ATP-binding protein, at 1,979,224 to 1,981,032
_c19060
PGA1_c19070: PGA1_c19070 - LysM domain., at 1,981,168 to 1,982,955
_c19070
PGA1_c19080: PGA1_c19080 - TIGR00730 family protein, at 1,983,121 to 1,983,663
_c19080
PGA1_c19090: PGA1_c19090 - Protein required for attachment to host cells, at 1,983,776 to 1,984,228
_c19090
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1980.198 kb on + strand, within PGA1_c19060 at 1980.212 kb on + strand, within PGA1_c19060 at 1980.218 kb on - strand, within PGA1_c19060 at 1980.383 kb on + strand, within PGA1_c19060 at 1980.677 kb on - strand, within PGA1_c19060 at 1980.741 kb on + strand, within PGA1_c19060 at 1980.781 kb on - strand, within PGA1_c19060 at 1980.854 kb on - strand at 1980.924 kb on + strand at 1980.934 kb on + strand at 1980.934 kb on + strand at 1980.942 kb on - strand at 1980.947 kb on + strand at 1980.947 kb on + strand at 1981.032 kb on + strand at 1981.060 kb on + strand at 1981.077 kb on + strand at 1981.092 kb on + strand at 1981.102 kb on - strand at 1981.135 kb on + strand at 1981.173 kb on + strand at 1981.184 kb on + strand at 1981.194 kb on + strand at 1981.207 kb on - strand at 1981.212 kb on - strand at 1981.214 kb on + strand at 1981.311 kb on + strand at 1981.319 kb on + strand at 1981.319 kb on - strand at 1981.447 kb on + strand, within PGA1_c19070 at 1981.454 kb on + strand, within PGA1_c19070 at 1981.462 kb on - strand, within PGA1_c19070 at 1981.521 kb on + strand, within PGA1_c19070 at 1981.521 kb on + strand, within PGA1_c19070 at 1981.529 kb on - strand, within PGA1_c19070 at 1981.529 kb on - strand, within PGA1_c19070 at 1981.573 kb on + strand, within PGA1_c19070 at 1981.573 kb on + strand, within PGA1_c19070 at 1981.625 kb on + strand, within PGA1_c19070 at 1981.631 kb on - strand, within PGA1_c19070 at 1981.633 kb on - strand, within PGA1_c19070 at 1981.667 kb on - strand, within PGA1_c19070 at 1981.694 kb on - strand, within PGA1_c19070 at 1981.694 kb on - strand, within PGA1_c19070 at 1981.710 kb on + strand, within PGA1_c19070 at 1981.772 kb on + strand, within PGA1_c19070 at 1981.859 kb on - strand, within PGA1_c19070 at 1981.925 kb on + strand, within PGA1_c19070 at 1981.925 kb on + strand, within PGA1_c19070 at 1981.933 kb on - strand, within PGA1_c19070 at 1981.933 kb on - strand, within PGA1_c19070 at 1981.933 kb on - strand, within PGA1_c19070 at 1981.949 kb on - strand, within PGA1_c19070 at 1981.956 kb on - strand, within PGA1_c19070 at 1982.013 kb on - strand, within PGA1_c19070 at 1982.186 kb on + strand, within PGA1_c19070 at 1982.243 kb on + strand, within PGA1_c19070 at 1982.243 kb on + strand, within PGA1_c19070 at 1982.286 kb on - strand, within PGA1_c19070 at 1982.287 kb on - strand, within PGA1_c19070 at 1982.356 kb on - strand, within PGA1_c19070 at 1982.364 kb on - strand, within PGA1_c19070 at 1982.371 kb on - strand, within PGA1_c19070 at 1982.418 kb on - strand, within PGA1_c19070 at 1982.420 kb on + strand, within PGA1_c19070 at 1982.430 kb on + strand, within PGA1_c19070 at 1982.462 kb on - strand, within PGA1_c19070 at 1982.551 kb on - strand, within PGA1_c19070 at 1982.703 kb on - strand, within PGA1_c19070 at 1982.719 kb on + strand, within PGA1_c19070 at 1982.886 kb on - strand at 1982.941 kb on - strand at 1983.014 kb on + strand at 1983.058 kb on - strand at 1983.242 kb on - strand, within PGA1_c19080 at 1983.243 kb on + strand, within PGA1_c19080 at 1983.250 kb on - strand, within PGA1_c19080 at 1983.296 kb on - strand, within PGA1_c19080 at 1983.299 kb on + strand, within PGA1_c19080 at 1983.313 kb on - strand, within PGA1_c19080 at 1983.373 kb on - strand, within PGA1_c19080 at 1983.411 kb on + strand, within PGA1_c19080 at 1983.433 kb on + strand, within PGA1_c19080 at 1983.491 kb on + strand, within PGA1_c19080 at 1983.491 kb on + strand, within PGA1_c19080 at 1983.491 kb on + strand, within PGA1_c19080 at 1983.557 kb on - strand, within PGA1_c19080 at 1983.719 kb on + strand at 1983.730 kb on - strand at 1983.773 kb on - strand at 1983.871 kb on - strand, within PGA1_c19090 at 1983.939 kb on + strand, within PGA1_c19090
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,980,198 + PGA1_c19060 0.54 +0.5 1,980,212 + PGA1_c19060 0.55 +1.3 1,980,218 - PGA1_c19060 0.55 +0.5 1,980,383 + PGA1_c19060 0.64 -0.5 1,980,677 - PGA1_c19060 0.80 -0.9 1,980,741 + PGA1_c19060 0.84 +1.9 1,980,781 - PGA1_c19060 0.86 +0.5 1,980,854 - -0.8 1,980,924 + -1.1 1,980,934 + -1.0 1,980,934 + +1.5 1,980,942 - -0.1 1,980,947 + +0.5 1,980,947 + +1.2 1,981,032 + +0.2 1,981,060 + -1.3 1,981,077 + -1.1 1,981,092 + -0.2 1,981,102 - -0.6 1,981,135 + -0.6 1,981,173 + +2.5 1,981,184 + -1.5 1,981,194 + -0.2 1,981,207 - +0.6 1,981,212 - -1.2 1,981,214 + -1.1 1,981,311 + +0.9 1,981,319 + +0.5 1,981,319 - +3.1 1,981,447 + PGA1_c19070 0.16 +1.9 1,981,454 + PGA1_c19070 0.16 -0.5 1,981,462 - PGA1_c19070 0.16 -1.3 1,981,521 + PGA1_c19070 0.20 +0.7 1,981,521 + PGA1_c19070 0.20 +0.9 1,981,529 - PGA1_c19070 0.20 +0.5 1,981,529 - PGA1_c19070 0.20 -1.1 1,981,573 + PGA1_c19070 0.23 +1.5 1,981,573 + PGA1_c19070 0.23 -0.4 1,981,625 + PGA1_c19070 0.26 -0.5 1,981,631 - PGA1_c19070 0.26 -0.1 1,981,633 - PGA1_c19070 0.26 -0.5 1,981,667 - PGA1_c19070 0.28 +0.4 1,981,694 - PGA1_c19070 0.29 +0.3 1,981,694 - PGA1_c19070 0.29 -0.4 1,981,710 + PGA1_c19070 0.30 -0.2 1,981,772 + PGA1_c19070 0.34 -0.1 1,981,859 - PGA1_c19070 0.39 -0.8 1,981,925 + PGA1_c19070 0.42 +1.0 1,981,925 + PGA1_c19070 0.42 -0.5 1,981,933 - PGA1_c19070 0.43 +0.1 1,981,933 - PGA1_c19070 0.43 +0.5 1,981,933 - PGA1_c19070 0.43 +0.0 1,981,949 - PGA1_c19070 0.44 -1.1 1,981,956 - PGA1_c19070 0.44 +0.8 1,982,013 - PGA1_c19070 0.47 +1.0 1,982,186 + PGA1_c19070 0.57 +0.0 1,982,243 + PGA1_c19070 0.60 -0.8 1,982,243 + PGA1_c19070 0.60 +1.5 1,982,286 - PGA1_c19070 0.63 +1.0 1,982,287 - PGA1_c19070 0.63 +0.4 1,982,356 - PGA1_c19070 0.66 +0.6 1,982,364 - PGA1_c19070 0.67 +1.0 1,982,371 - PGA1_c19070 0.67 -0.1 1,982,418 - PGA1_c19070 0.70 -0.3 1,982,420 + PGA1_c19070 0.70 +1.1 1,982,430 + PGA1_c19070 0.71 -2.0 1,982,462 - PGA1_c19070 0.72 -1.1 1,982,551 - PGA1_c19070 0.77 -0.3 1,982,703 - PGA1_c19070 0.86 -0.1 1,982,719 + PGA1_c19070 0.87 +1.0 1,982,886 - -1.5 1,982,941 - -0.5 1,983,014 + +1.5 1,983,058 - +0.9 1,983,242 - PGA1_c19080 0.22 -0.5 1,983,243 + PGA1_c19080 0.22 -0.1 1,983,250 - PGA1_c19080 0.24 -0.5 1,983,296 - PGA1_c19080 0.32 -0.5 1,983,299 + PGA1_c19080 0.33 -0.1 1,983,313 - PGA1_c19080 0.35 +0.7 1,983,373 - PGA1_c19080 0.46 +0.7 1,983,411 + PGA1_c19080 0.53 +1.5 1,983,433 + PGA1_c19080 0.57 +0.9 1,983,491 + PGA1_c19080 0.68 -1.6 1,983,491 + PGA1_c19080 0.68 +0.3 1,983,491 + PGA1_c19080 0.68 +1.1 1,983,557 - PGA1_c19080 0.80 -0.5 1,983,719 + +0.8 1,983,730 - +0.5 1,983,773 - -0.2 1,983,871 - PGA1_c19090 0.21 +2.2 1,983,939 + PGA1_c19090 0.36 -0.8
Or see this region's nucleotide sequence