Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16200 and PGA1_c16210 overlap by 4 nucleotides PGA1_c16210 and PGA1_c16220 overlap by 1 nucleotides
PGA1_c16200: PGA1_c16200 - molybdopterin dehydrogenase, FAD-binding, at 1,681,853 to 1,682,719
_c16200
PGA1_c16210: PGA1_c16210 - iron-sulfur cluster binding domain-containing protein, at 1,682,716 to 1,683,198
_c16210
PGA1_c16220: PGA1_c16220 - molybdopterin binding domain-containing protein, at 1,683,198 to 1,685,468
_c16220
Position (kb)
1682
1683
1684 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1681.747 kb on + strand at 1681.747 kb on + strand at 1681.836 kb on + strand at 1681.913 kb on + strand at 1681.914 kb on - strand at 1681.920 kb on + strand at 1681.921 kb on - strand at 1681.928 kb on - strand at 1682.091 kb on + strand, within PGA1_c16200 at 1682.098 kb on + strand, within PGA1_c16200 at 1682.113 kb on + strand, within PGA1_c16200 at 1682.135 kb on + strand, within PGA1_c16200 at 1682.156 kb on + strand, within PGA1_c16200 at 1682.156 kb on + strand, within PGA1_c16200 at 1682.164 kb on - strand, within PGA1_c16200 at 1682.188 kb on + strand, within PGA1_c16200 at 1682.204 kb on + strand, within PGA1_c16200 at 1682.204 kb on + strand, within PGA1_c16200 at 1682.212 kb on - strand, within PGA1_c16200 at 1682.267 kb on - strand, within PGA1_c16200 at 1682.285 kb on + strand, within PGA1_c16200 at 1682.338 kb on + strand, within PGA1_c16200 at 1682.338 kb on + strand, within PGA1_c16200 at 1682.338 kb on + strand, within PGA1_c16200 at 1682.351 kb on - strand, within PGA1_c16200 at 1682.372 kb on + strand, within PGA1_c16200 at 1682.372 kb on + strand, within PGA1_c16200 at 1682.380 kb on - strand, within PGA1_c16200 at 1682.380 kb on - strand, within PGA1_c16200 at 1682.380 kb on - strand, within PGA1_c16200 at 1682.380 kb on - strand, within PGA1_c16200 at 1682.414 kb on - strand, within PGA1_c16200 at 1682.426 kb on + strand, within PGA1_c16200 at 1682.524 kb on + strand, within PGA1_c16200 at 1682.524 kb on + strand, within PGA1_c16200 at 1682.524 kb on + strand, within PGA1_c16200 at 1682.529 kb on + strand, within PGA1_c16200 at 1682.532 kb on - strand, within PGA1_c16200 at 1682.581 kb on + strand, within PGA1_c16200 at 1682.695 kb on - strand at 1682.712 kb on + strand at 1682.905 kb on - strand, within PGA1_c16210 at 1682.923 kb on - strand, within PGA1_c16210 at 1683.050 kb on + strand, within PGA1_c16210 at 1683.120 kb on + strand, within PGA1_c16210 at 1683.230 kb on - strand at 1683.234 kb on - strand at 1683.269 kb on - strand at 1683.295 kb on - strand at 1683.323 kb on + strand at 1683.388 kb on - strand at 1683.414 kb on + strand at 1683.422 kb on - strand at 1683.476 kb on + strand, within PGA1_c16220 at 1683.551 kb on - strand, within PGA1_c16220 at 1683.561 kb on - strand, within PGA1_c16220 at 1683.561 kb on - strand, within PGA1_c16220 at 1683.561 kb on - strand, within PGA1_c16220 at 1683.577 kb on + strand, within PGA1_c16220 at 1683.587 kb on + strand, within PGA1_c16220 at 1683.647 kb on + strand, within PGA1_c16220 at 1683.685 kb on - strand, within PGA1_c16220 at 1683.712 kb on - strand, within PGA1_c16220 at 1683.769 kb on + strand, within PGA1_c16220 at 1683.807 kb on + strand, within PGA1_c16220 at 1683.807 kb on + strand, within PGA1_c16220 at 1683.812 kb on + strand, within PGA1_c16220 at 1683.812 kb on + strand, within PGA1_c16220 at 1683.815 kb on - strand, within PGA1_c16220 at 1683.820 kb on - strand, within PGA1_c16220 at 1683.825 kb on + strand, within PGA1_c16220 at 1683.870 kb on + strand, within PGA1_c16220 at 1683.870 kb on + strand, within PGA1_c16220 at 1683.880 kb on - strand, within PGA1_c16220 at 1683.888 kb on + strand, within PGA1_c16220 at 1683.888 kb on + strand, within PGA1_c16220 at 1683.913 kb on + strand, within PGA1_c16220 at 1683.937 kb on + strand, within PGA1_c16220 at 1683.937 kb on + strand, within PGA1_c16220 at 1683.945 kb on - strand, within PGA1_c16220 at 1683.945 kb on - strand, within PGA1_c16220 at 1683.945 kb on - strand, within PGA1_c16220 at 1684.016 kb on - strand, within PGA1_c16220 at 1684.023 kb on - strand, within PGA1_c16220 at 1684.074 kb on - strand, within PGA1_c16220 at 1684.074 kb on - strand, within PGA1_c16220 at 1684.074 kb on - strand, within PGA1_c16220 at 1684.079 kb on - strand, within PGA1_c16220 at 1684.106 kb on - strand, within PGA1_c16220 at 1684.155 kb on + strand, within PGA1_c16220 at 1684.158 kb on - strand, within PGA1_c16220 at 1684.158 kb on - strand, within PGA1_c16220 at 1684.165 kb on + strand, within PGA1_c16220 at 1684.165 kb on + strand, within PGA1_c16220 at 1684.173 kb on - strand, within PGA1_c16220
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,681,747 + +1.5 1,681,747 + +0.2 1,681,836 + -1.2 1,681,913 + -0.5 1,681,914 - -0.4 1,681,920 + +0.2 1,681,921 - +0.5 1,681,928 - +0.3 1,682,091 + PGA1_c16200 0.27 +0.3 1,682,098 + PGA1_c16200 0.28 +1.5 1,682,113 + PGA1_c16200 0.30 -0.2 1,682,135 + PGA1_c16200 0.33 -0.6 1,682,156 + PGA1_c16200 0.35 -0.5 1,682,156 + PGA1_c16200 0.35 -0.9 1,682,164 - PGA1_c16200 0.36 +0.1 1,682,188 + PGA1_c16200 0.39 +0.3 1,682,204 + PGA1_c16200 0.40 +0.5 1,682,204 + PGA1_c16200 0.40 -1.2 1,682,212 - PGA1_c16200 0.41 -0.9 1,682,267 - PGA1_c16200 0.48 -0.9 1,682,285 + PGA1_c16200 0.50 +0.9 1,682,338 + PGA1_c16200 0.56 -0.1 1,682,338 + PGA1_c16200 0.56 +0.0 1,682,338 + PGA1_c16200 0.56 -0.1 1,682,351 - PGA1_c16200 0.57 -1.5 1,682,372 + PGA1_c16200 0.60 -1.4 1,682,372 + PGA1_c16200 0.60 -0.5 1,682,380 - PGA1_c16200 0.61 -0.4 1,682,380 - PGA1_c16200 0.61 -2.2 1,682,380 - PGA1_c16200 0.61 -0.0 1,682,380 - PGA1_c16200 0.61 +0.9 1,682,414 - PGA1_c16200 0.65 +2.0 1,682,426 + PGA1_c16200 0.66 -0.1 1,682,524 + PGA1_c16200 0.77 -0.5 1,682,524 + PGA1_c16200 0.77 +0.3 1,682,524 + PGA1_c16200 0.77 -0.5 1,682,529 + PGA1_c16200 0.78 +0.1 1,682,532 - PGA1_c16200 0.78 -0.7 1,682,581 + PGA1_c16200 0.84 +0.1 1,682,695 - -0.6 1,682,712 + +1.5 1,682,905 - PGA1_c16210 0.39 +0.1 1,682,923 - PGA1_c16210 0.43 -0.7 1,683,050 + PGA1_c16210 0.69 +0.5 1,683,120 + PGA1_c16210 0.84 -1.3 1,683,230 - -0.1 1,683,234 - -1.5 1,683,269 - -0.1 1,683,295 - +0.5 1,683,323 + +0.8 1,683,388 - -0.5 1,683,414 + +0.0 1,683,422 - -0.3 1,683,476 + PGA1_c16220 0.12 +0.1 1,683,551 - PGA1_c16220 0.16 +0.7 1,683,561 - PGA1_c16220 0.16 +0.5 1,683,561 - PGA1_c16220 0.16 +0.5 1,683,561 - PGA1_c16220 0.16 -1.1 1,683,577 + PGA1_c16220 0.17 -0.1 1,683,587 + PGA1_c16220 0.17 -0.7 1,683,647 + PGA1_c16220 0.20 -0.9 1,683,685 - PGA1_c16220 0.21 +0.2 1,683,712 - PGA1_c16220 0.23 -1.1 1,683,769 + PGA1_c16220 0.25 -1.0 1,683,807 + PGA1_c16220 0.27 -0.2 1,683,807 + PGA1_c16220 0.27 -0.8 1,683,812 + PGA1_c16220 0.27 -0.7 1,683,812 + PGA1_c16220 0.27 -2.8 1,683,815 - PGA1_c16220 0.27 -1.0 1,683,820 - PGA1_c16220 0.27 +1.1 1,683,825 + PGA1_c16220 0.28 -0.1 1,683,870 + PGA1_c16220 0.30 -0.4 1,683,870 + PGA1_c16220 0.30 -0.7 1,683,880 - PGA1_c16220 0.30 -0.3 1,683,888 + PGA1_c16220 0.30 -1.0 1,683,888 + PGA1_c16220 0.30 -1.1 1,683,913 + PGA1_c16220 0.31 -0.5 1,683,937 + PGA1_c16220 0.33 +0.3 1,683,937 + PGA1_c16220 0.33 -0.8 1,683,945 - PGA1_c16220 0.33 +0.1 1,683,945 - PGA1_c16220 0.33 +0.5 1,683,945 - PGA1_c16220 0.33 -0.5 1,684,016 - PGA1_c16220 0.36 -1.1 1,684,023 - PGA1_c16220 0.36 +1.7 1,684,074 - PGA1_c16220 0.39 -0.9 1,684,074 - PGA1_c16220 0.39 -0.5 1,684,074 - PGA1_c16220 0.39 +0.5 1,684,079 - PGA1_c16220 0.39 +0.2 1,684,106 - PGA1_c16220 0.40 +1.1 1,684,155 + PGA1_c16220 0.42 -0.7 1,684,158 - PGA1_c16220 0.42 -1.7 1,684,158 - PGA1_c16220 0.42 +0.5 1,684,165 + PGA1_c16220 0.43 +0.0 1,684,165 + PGA1_c16220 0.43 +1.8 1,684,173 - PGA1_c16220 0.43 -0.9
Or see this region's nucleotide sequence