Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16170 and PGA1_c16180 are separated by 178 nucleotides PGA1_c16180 and PGA1_c16190 are separated by 320 nucleotides PGA1_c16190 and PGA1_c16200 are separated by 198 nucleotides
PGA1_c16170: PGA1_c16170 - TRAP transporter, 4TM/12TM fusion protein, at 1,677,401 to 1,680,010
_c16170
PGA1_c16180: PGA1_c16180 - TRAP transporter solute receptor, TAXI family, at 1,680,189 to 1,681,154
_c16180
PGA1_c16190: PGA1_c16190 - hypothetical protein, at 1,681,475 to 1,681,654
_c16190
PGA1_c16200: PGA1_c16200 - molybdopterin dehydrogenase, FAD-binding, at 1,681,853 to 1,682,719
_c16200
Position (kb)
1680
1681
1682 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1679.261 kb on - strand, within PGA1_c16170 at 1679.283 kb on + strand, within PGA1_c16170 at 1679.283 kb on + strand, within PGA1_c16170 at 1679.301 kb on - strand, within PGA1_c16170 at 1679.334 kb on + strand, within PGA1_c16170 at 1679.342 kb on - strand, within PGA1_c16170 at 1679.438 kb on - strand, within PGA1_c16170 at 1679.477 kb on + strand, within PGA1_c16170 at 1679.556 kb on - strand, within PGA1_c16170 at 1679.556 kb on - strand, within PGA1_c16170 at 1679.621 kb on + strand, within PGA1_c16170 at 1679.714 kb on - strand, within PGA1_c16170 at 1679.836 kb on + strand at 1679.852 kb on + strand at 1679.862 kb on + strand at 1680.058 kb on + strand at 1680.119 kb on + strand at 1680.120 kb on + strand at 1680.166 kb on + strand at 1680.176 kb on + strand at 1680.176 kb on + strand at 1680.194 kb on - strand at 1680.196 kb on + strand at 1680.196 kb on + strand at 1680.199 kb on - strand at 1680.209 kb on - strand at 1680.274 kb on + strand at 1680.282 kb on - strand at 1680.289 kb on + strand, within PGA1_c16180 at 1680.341 kb on + strand, within PGA1_c16180 at 1680.342 kb on - strand, within PGA1_c16180 at 1680.388 kb on + strand, within PGA1_c16180 at 1680.574 kb on + strand, within PGA1_c16180 at 1680.574 kb on + strand, within PGA1_c16180 at 1680.574 kb on + strand, within PGA1_c16180 at 1680.582 kb on - strand, within PGA1_c16180 at 1680.582 kb on - strand, within PGA1_c16180 at 1680.604 kb on - strand, within PGA1_c16180 at 1680.689 kb on - strand, within PGA1_c16180 at 1680.799 kb on + strand, within PGA1_c16180 at 1680.950 kb on - strand, within PGA1_c16180 at 1681.094 kb on - strand at 1681.139 kb on + strand at 1681.149 kb on + strand at 1681.157 kb on - strand at 1681.157 kb on - strand at 1681.158 kb on - strand at 1681.227 kb on - strand at 1681.289 kb on + strand at 1681.296 kb on - strand at 1681.323 kb on - strand at 1681.325 kb on + strand at 1681.326 kb on + strand at 1681.333 kb on - strand at 1681.333 kb on - strand at 1681.366 kb on - strand at 1681.372 kb on - strand at 1681.423 kb on - strand at 1681.468 kb on - strand at 1681.473 kb on + strand at 1681.535 kb on - strand, within PGA1_c16190 at 1681.535 kb on - strand, within PGA1_c16190 at 1681.557 kb on - strand, within PGA1_c16190 at 1681.713 kb on - strand at 1681.747 kb on + strand at 1681.747 kb on + strand at 1681.836 kb on + strand at 1681.913 kb on + strand at 1681.914 kb on - strand at 1681.920 kb on + strand at 1681.921 kb on - strand at 1681.928 kb on - strand at 1682.091 kb on + strand, within PGA1_c16200 at 1682.098 kb on + strand, within PGA1_c16200 at 1682.113 kb on + strand, within PGA1_c16200 at 1682.135 kb on + strand, within PGA1_c16200
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,679,261 - PGA1_c16170 0.71 -0.1 1,679,283 + PGA1_c16170 0.72 -1.4 1,679,283 + PGA1_c16170 0.72 +0.8 1,679,301 - PGA1_c16170 0.73 -0.1 1,679,334 + PGA1_c16170 0.74 +1.5 1,679,342 - PGA1_c16170 0.74 +0.5 1,679,438 - PGA1_c16170 0.78 +0.1 1,679,477 + PGA1_c16170 0.80 +0.5 1,679,556 - PGA1_c16170 0.83 +0.6 1,679,556 - PGA1_c16170 0.83 -0.9 1,679,621 + PGA1_c16170 0.85 -0.9 1,679,714 - PGA1_c16170 0.89 +1.2 1,679,836 + -1.4 1,679,852 + -1.4 1,679,862 + -0.9 1,680,058 + +1.5 1,680,119 + +0.9 1,680,120 + -0.9 1,680,166 + -0.7 1,680,176 + -1.4 1,680,176 + -0.1 1,680,194 - +0.8 1,680,196 + +1.0 1,680,196 + -1.4 1,680,199 - -0.1 1,680,209 - +0.5 1,680,274 + +0.1 1,680,282 - -0.1 1,680,289 + PGA1_c16180 0.10 +0.2 1,680,341 + PGA1_c16180 0.16 -1.1 1,680,342 - PGA1_c16180 0.16 +1.9 1,680,388 + PGA1_c16180 0.21 +0.1 1,680,574 + PGA1_c16180 0.40 +0.0 1,680,574 + PGA1_c16180 0.40 -1.1 1,680,574 + PGA1_c16180 0.40 -2.2 1,680,582 - PGA1_c16180 0.41 -1.1 1,680,582 - PGA1_c16180 0.41 +0.1 1,680,604 - PGA1_c16180 0.43 -0.9 1,680,689 - PGA1_c16180 0.52 -0.1 1,680,799 + PGA1_c16180 0.63 +0.5 1,680,950 - PGA1_c16180 0.79 -1.5 1,681,094 - -0.1 1,681,139 + +0.5 1,681,149 + -0.2 1,681,157 - -0.9 1,681,157 - +0.5 1,681,158 - -0.1 1,681,227 - -2.0 1,681,289 + -0.5 1,681,296 - -0.5 1,681,323 - -0.7 1,681,325 + -2.0 1,681,326 + -0.7 1,681,333 - -0.8 1,681,333 - +0.5 1,681,366 - -0.4 1,681,372 - -1.0 1,681,423 - -0.3 1,681,468 - +0.2 1,681,473 + +0.9 1,681,535 - PGA1_c16190 0.33 -2.2 1,681,535 - PGA1_c16190 0.33 -0.7 1,681,557 - PGA1_c16190 0.46 -0.5 1,681,713 - +0.5 1,681,747 + +1.5 1,681,747 + +0.2 1,681,836 + -1.2 1,681,913 + -0.5 1,681,914 - -0.4 1,681,920 + +0.2 1,681,921 - +0.5 1,681,928 - +0.3 1,682,091 + PGA1_c16200 0.27 +0.3 1,682,098 + PGA1_c16200 0.28 +1.5 1,682,113 + PGA1_c16200 0.30 -0.2 1,682,135 + PGA1_c16200 0.33 -0.6
Or see this region's nucleotide sequence