Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14380 and PGA1_c14390 are separated by 603 nucleotides PGA1_c14390 and PGA1_c14400 are separated by 59 nucleotides PGA1_c14400 and PGA1_c14410 are separated by 56 nucleotides
PGA1_c14380: PGA1_c14380 - ATP-dependent DNA helicase RecG, at 1,493,686 to 1,495,776
_c14380
PGA1_c14390: PGA1_c14390 - NifU homolog involved in Fe-S cluster formation, at 1,496,380 to 1,496,832
_c14390
PGA1_c14400: PGA1_c14400 - phosphoribosyl-AMP cyclohydrolase Hisl, at 1,496,892 to 1,497,269
_c14400
PGA1_c14410: PGA1_c14410 - putative glutamyl-tRNA synthetase, at 1,497,326 to 1,498,201
_c14410
Position (kb)
1496
1497 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1495.438 kb on + strand, within PGA1_c14380 at 1495.542 kb on - strand, within PGA1_c14380 at 1495.633 kb on + strand at 1495.743 kb on + strand at 1495.779 kb on - strand at 1495.787 kb on + strand at 1495.787 kb on + strand at 1495.787 kb on + strand at 1495.795 kb on - strand at 1495.885 kb on + strand at 1495.991 kb on + strand at 1496.064 kb on + strand at 1496.083 kb on + strand at 1496.084 kb on + strand at 1496.160 kb on + strand at 1496.160 kb on + strand at 1496.242 kb on + strand at 1496.259 kb on + strand at 1496.261 kb on + strand at 1496.267 kb on - strand at 1496.618 kb on + strand, within PGA1_c14390 at 1496.766 kb on + strand, within PGA1_c14390 at 1496.790 kb on + strand at 1496.814 kb on + strand at 1496.822 kb on - strand at 1496.829 kb on + strand at 1497.240 kb on + strand at 1497.263 kb on + strand at 1497.263 kb on + strand at 1497.263 kb on + strand at 1497.263 kb on + strand at 1497.264 kb on + strand at 1497.271 kb on - strand at 1497.359 kb on - strand at 1497.388 kb on + strand at 1497.550 kb on + strand, within PGA1_c14410 at 1497.621 kb on - strand, within PGA1_c14410 at 1497.623 kb on + strand, within PGA1_c14410 at 1497.631 kb on - strand, within PGA1_c14410 at 1497.668 kb on - strand, within PGA1_c14410
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,495,438 + PGA1_c14380 0.84 -1.5 1,495,542 - PGA1_c14380 0.89 +2.0 1,495,633 + -0.6 1,495,743 + +0.7 1,495,779 - +1.4 1,495,787 + -1.2 1,495,787 + +0.8 1,495,787 + -1.9 1,495,795 - -0.2 1,495,885 + -0.2 1,495,991 + +2.4 1,496,064 + -1.2 1,496,083 + +0.2 1,496,084 + +1.2 1,496,160 + -1.2 1,496,160 + -1.2 1,496,242 + -0.0 1,496,259 + -1.9 1,496,261 + +0.1 1,496,267 - +0.8 1,496,618 + PGA1_c14390 0.53 +0.8 1,496,766 + PGA1_c14390 0.85 +0.4 1,496,790 + -1.2 1,496,814 + -1.2 1,496,822 - +0.0 1,496,829 + -0.6 1,497,240 + -0.9 1,497,263 + -2.3 1,497,263 + +1.7 1,497,263 + -0.4 1,497,263 + -0.7 1,497,264 + -0.5 1,497,271 - -0.0 1,497,359 - +1.4 1,497,388 + +1.4 1,497,550 + PGA1_c14410 0.26 -0.6 1,497,621 - PGA1_c14410 0.34 +1.0 1,497,623 + PGA1_c14410 0.34 -0.6 1,497,631 - PGA1_c14410 0.35 -1.5 1,497,668 - PGA1_c14410 0.39 -0.6
Or see this region's nucleotide sequence