Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13460 and PGA1_c13470 are separated by 93 nucleotides PGA1_c13470 and PGA1_c13480 are separated by 131 nucleotides PGA1_c13480 and PGA1_c13490 overlap by 1 nucleotides
PGA1_c13460: PGA1_c13460 - putative sulfurtransferase, at 1,393,331 to 1,393,564
_c13460
PGA1_c13470: PGA1_c13470 - cytochrome C biogenesis protein, transmembrane region, at 1,393,658 to 1,394,410
_c13470
PGA1_c13480: PGA1_c13480 - hypothetical protein, at 1,394,542 to 1,395,870
_c13480
PGA1_c13490: PGA1_c13490 - putative cytochrome P450, at 1,395,870 to 1,397,300
_c13490
Position (kb)
1394
1395
1396 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1393.567 kb on - strand at 1393.585 kb on + strand at 1393.585 kb on + strand at 1393.595 kb on + strand at 1393.603 kb on - strand at 1393.603 kb on - strand at 1393.604 kb on + strand at 1393.604 kb on + strand at 1393.604 kb on + strand at 1393.612 kb on - strand at 1393.612 kb on - strand at 1393.612 kb on - strand at 1393.612 kb on - strand at 1393.612 kb on - strand at 1393.612 kb on - strand at 1393.642 kb on + strand at 1393.642 kb on + strand at 1393.642 kb on + strand at 1393.642 kb on + strand at 1393.642 kb on + strand at 1393.642 kb on + strand at 1393.643 kb on + strand at 1394.412 kb on - strand at 1394.419 kb on + strand at 1394.443 kb on + strand at 1394.443 kb on + strand at 1394.443 kb on + strand at 1394.451 kb on - strand at 1394.451 kb on - strand at 1394.451 kb on - strand at 1394.451 kb on - strand at 1394.451 kb on - strand at 1394.518 kb on - strand at 1394.658 kb on + strand at 1394.678 kb on + strand, within PGA1_c13480 at 1394.780 kb on - strand, within PGA1_c13480 at 1394.858 kb on - strand, within PGA1_c13480 at 1394.866 kb on + strand, within PGA1_c13480 at 1394.866 kb on + strand, within PGA1_c13480 at 1394.871 kb on + strand, within PGA1_c13480 at 1394.884 kb on - strand, within PGA1_c13480 at 1394.970 kb on + strand, within PGA1_c13480 at 1394.994 kb on + strand, within PGA1_c13480 at 1395.028 kb on + strand, within PGA1_c13480 at 1395.036 kb on - strand, within PGA1_c13480 at 1395.036 kb on - strand, within PGA1_c13480 at 1395.072 kb on + strand, within PGA1_c13480 at 1395.072 kb on + strand, within PGA1_c13480 at 1395.094 kb on - strand, within PGA1_c13480 at 1395.094 kb on - strand, within PGA1_c13480 at 1395.094 kb on - strand, within PGA1_c13480 at 1395.241 kb on + strand, within PGA1_c13480 at 1395.259 kb on - strand, within PGA1_c13480 at 1395.259 kb on - strand, within PGA1_c13480 at 1395.266 kb on + strand, within PGA1_c13480 at 1395.274 kb on - strand, within PGA1_c13480 at 1395.572 kb on + strand, within PGA1_c13480 at 1395.590 kb on - strand, within PGA1_c13480 at 1395.690 kb on + strand, within PGA1_c13480 at 1395.693 kb on - strand, within PGA1_c13480 at 1395.840 kb on - strand at 1395.875 kb on - strand at 1395.931 kb on + strand at 1395.933 kb on + strand at 1395.933 kb on + strand at 1396.011 kb on + strand at 1396.037 kb on + strand, within PGA1_c13490 at 1396.037 kb on + strand, within PGA1_c13490 at 1396.079 kb on - strand, within PGA1_c13490 at 1396.112 kb on - strand, within PGA1_c13490 at 1396.112 kb on - strand, within PGA1_c13490 at 1396.119 kb on + strand, within PGA1_c13490 at 1396.170 kb on + strand, within PGA1_c13490 at 1396.170 kb on + strand, within PGA1_c13490 at 1396.171 kb on + strand, within PGA1_c13490 at 1396.173 kb on - strand, within PGA1_c13490 at 1396.311 kb on + strand, within PGA1_c13490 at 1396.311 kb on + strand, within PGA1_c13490 at 1396.311 kb on + strand, within PGA1_c13490 at 1396.327 kb on + strand, within PGA1_c13490 at 1396.369 kb on + strand, within PGA1_c13490 at 1396.537 kb on + strand, within PGA1_c13490 at 1396.545 kb on - strand, within PGA1_c13490 at 1396.618 kb on - strand, within PGA1_c13490 at 1396.658 kb on + strand, within PGA1_c13490 at 1396.658 kb on + strand, within PGA1_c13490 at 1396.694 kb on + strand, within PGA1_c13490 at 1396.694 kb on + strand, within PGA1_c13490 at 1396.702 kb on - strand, within PGA1_c13490 at 1396.702 kb on - strand, within PGA1_c13490 at 1396.707 kb on - strand, within PGA1_c13490 at 1396.746 kb on + strand, within PGA1_c13490 at 1396.759 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.764 kb on + strand, within PGA1_c13490 at 1396.769 kb on + strand, within PGA1_c13490 at 1396.769 kb on + strand, within PGA1_c13490 at 1396.769 kb on + strand, within PGA1_c13490 at 1396.769 kb on + strand, within PGA1_c13490 at 1396.769 kb on + strand, within PGA1_c13490 at 1396.772 kb on - strand, within PGA1_c13490 at 1396.834 kb on - strand, within PGA1_c13490
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,393,567 - +0.6 1,393,585 + -2.2 1,393,585 + +0.1 1,393,595 + -0.1 1,393,603 - +0.3 1,393,603 - +0.5 1,393,604 + -2.1 1,393,604 + +1.5 1,393,604 + +1.2 1,393,612 - -0.7 1,393,612 - +1.7 1,393,612 - -1.5 1,393,612 - -0.8 1,393,612 - -0.4 1,393,612 - +0.7 1,393,642 + +1.9 1,393,642 + -0.5 1,393,642 + +0.9 1,393,642 + -0.1 1,393,642 + +0.5 1,393,642 + -0.4 1,393,643 + +0.8 1,394,412 - +0.9 1,394,419 + -0.2 1,394,443 + +0.5 1,394,443 + +0.2 1,394,443 + +1.5 1,394,451 - +0.5 1,394,451 - -0.1 1,394,451 - -1.5 1,394,451 - +1.8 1,394,451 - +0.6 1,394,518 - -0.5 1,394,658 + -1.9 1,394,678 + PGA1_c13480 0.10 +0.3 1,394,780 - PGA1_c13480 0.18 -0.5 1,394,858 - PGA1_c13480 0.24 -0.8 1,394,866 + PGA1_c13480 0.24 -0.6 1,394,866 + PGA1_c13480 0.24 -2.0 1,394,871 + PGA1_c13480 0.25 -0.1 1,394,884 - PGA1_c13480 0.26 -1.6 1,394,970 + PGA1_c13480 0.32 -1.8 1,394,994 + PGA1_c13480 0.34 +0.1 1,395,028 + PGA1_c13480 0.37 -0.9 1,395,036 - PGA1_c13480 0.37 -1.3 1,395,036 - PGA1_c13480 0.37 -1.8 1,395,072 + PGA1_c13480 0.40 +0.1 1,395,072 + PGA1_c13480 0.40 -1.2 1,395,094 - PGA1_c13480 0.42 -3.3 1,395,094 - PGA1_c13480 0.42 -2.1 1,395,094 - PGA1_c13480 0.42 +0.5 1,395,241 + PGA1_c13480 0.53 +1.5 1,395,259 - PGA1_c13480 0.54 -0.9 1,395,259 - PGA1_c13480 0.54 -1.0 1,395,266 + PGA1_c13480 0.54 -1.8 1,395,274 - PGA1_c13480 0.55 -1.7 1,395,572 + PGA1_c13480 0.78 -1.5 1,395,590 - PGA1_c13480 0.79 -0.5 1,395,690 + PGA1_c13480 0.86 -1.7 1,395,693 - PGA1_c13480 0.87 -2.4 1,395,840 - -1.2 1,395,875 - -0.1 1,395,931 + +0.5 1,395,933 + -1.4 1,395,933 + -0.1 1,396,011 + -0.5 1,396,037 + PGA1_c13490 0.12 -0.2 1,396,037 + PGA1_c13490 0.12 -1.4 1,396,079 - PGA1_c13490 0.15 -0.4 1,396,112 - PGA1_c13490 0.17 -0.7 1,396,112 - PGA1_c13490 0.17 -0.6 1,396,119 + PGA1_c13490 0.17 -0.8 1,396,170 + PGA1_c13490 0.21 -0.7 1,396,170 + PGA1_c13490 0.21 -0.3 1,396,171 + PGA1_c13490 0.21 +0.3 1,396,173 - PGA1_c13490 0.21 +0.6 1,396,311 + PGA1_c13490 0.31 +1.5 1,396,311 + PGA1_c13490 0.31 -1.5 1,396,311 + PGA1_c13490 0.31 -1.2 1,396,327 + PGA1_c13490 0.32 +1.5 1,396,369 + PGA1_c13490 0.35 +1.1 1,396,537 + PGA1_c13490 0.47 -1.7 1,396,545 - PGA1_c13490 0.47 -0.1 1,396,618 - PGA1_c13490 0.52 -1.1 1,396,658 + PGA1_c13490 0.55 -1.0 1,396,658 + PGA1_c13490 0.55 -1.7 1,396,694 + PGA1_c13490 0.58 -2.7 1,396,694 + PGA1_c13490 0.58 +0.5 1,396,702 - PGA1_c13490 0.58 +0.6 1,396,702 - PGA1_c13490 0.58 +3.5 1,396,707 - PGA1_c13490 0.58 +0.2 1,396,746 + PGA1_c13490 0.61 -1.5 1,396,759 + PGA1_c13490 0.62 -0.4 1,396,764 + PGA1_c13490 0.62 -1.1 1,396,764 + PGA1_c13490 0.62 -1.5 1,396,764 + PGA1_c13490 0.62 -2.5 1,396,764 + PGA1_c13490 0.62 -1.2 1,396,764 + PGA1_c13490 0.62 +1.0 1,396,764 + PGA1_c13490 0.62 -0.1 1,396,764 + PGA1_c13490 0.62 +0.7 1,396,769 + PGA1_c13490 0.63 +0.1 1,396,769 + PGA1_c13490 0.63 -0.7 1,396,769 + PGA1_c13490 0.63 -1.1 1,396,769 + PGA1_c13490 0.63 +0.2 1,396,769 + PGA1_c13490 0.63 -0.8 1,396,772 - PGA1_c13490 0.63 -2.1 1,396,834 - PGA1_c13490 0.67 -0.4
Or see this region's nucleotide sequence